2014, volume:8 issue:3

Comparison Between the Efficiency of nested PCR Analysis and IgG Avidity Test in Detection Of Acute Toxoplasma gondii Infection in Early Pregnancy in Ramadi city.

Journal of University of Anbar for Pure Science , volume:8 issue:3

Background: Primary maternal infection with toxoplasmosis during gestation and its transmission to the fetus continue to be the cause of tragic yet preventable disease in offspring. jective: This study was aimed to Comparison between efficiency of these two methods(nested PCR and IgG avidity test) in detection of acute toxoplasmosis in pregnant women early in the first trimester is of utmost importance in order to offer them early therapy or other interventions to prevent congenital infection of fetuses. Methods: One hundred sixteen blood samples were collected from pregnant women at different ages, and in the first trimester of pregnancy. These women have history of habitual abortion, intrauterine fetal death and congenital anomalies of the fetus. Blood samples were tested for specific anti-Toxoplasma IgG avidity test and detection of B1 gene of T. gondii by nPCR. Results: In this study, it was found out that the rate of Toxoplasmosis by nested PCR 48 cases (41.4%),while by T. IgG avidity test was 76cases(65.5%) (high avidity 54.3% and low avidity11.2%). The detection rate by nested PCR was significantly higher than by T. IgG avidity test. Conclusion: PCR technique is more sensitive and specific than T.IgG avidity tests.

The effect of some organic substances and the concentration of dayizone pesticide on the composition and microbial activity of soils

Ahmed Mohammed turkey

Journal of University of Anbar for Pure Science , volume:8 issue:3

The present study has been conducted to investigate the effect of organic substance of klkoz and the clover added to the soil on the decomposition of dayizon pesticide in the soil , and the effect of this on the microbial activity of microorganisms in addition of the organic Carbon ,Nitrogen and Phosphorus in the soil –A type of mixed soil was used in the study – It was brought from an agricultural field in the city of Ramadi in which no type of pesticides had been used for a period of more than ten years . The organic substance of klkoz and the dry clover remaining had been added and cultivated well in the soil at a ratio of %1 for all treatments. Three concentrations(0, 250,500) ML.100L. Donam had been added to the soil according to the treatment. All these treatment had been incubated at a tempreture%28 for(20,40,60)days. Then, the total number of microbe, fungi, Actinomycetes ,Bacteria solvent for phosphate ,cellulose decomposing Bacteria, Aozossberellim Bacteria , and the Azutobkatr Bacteria were estimated in the soil .Besides, the Nitrogen, the organic carbon, and phosphor , were also estimated in the soil under study .The results revealed that the addition of the organic substance has a great role in the increase of different races of microorganism in the soil and in the decrease in the inhibitory effect of pesticide under study . It was indicated that there was a differentiated effect of adding an organic substance to the soil at %1. This led to an increase in the total numbers of the treatment that used a pesticide . The numbers reached the higher level after a period of incubation of (60) days on the other hand , when the pesticide used without an organic substance , the effect was higher that the total numbers of microbe decreased differently according to the difference in the concentration used and the period of incubation , so that difference between the treatment of comparison and au treatments of the pesticide was significant from the period of incubation (20) days until the end of the experiment (60) days . This recorded the less effect of the recommended dose of the used pesticide on the other hand the effect was inhibitory , signification and clean when using a double dose of pesticide recommended in the field. The decrease in the numbers as a result of using the pesticide with the existence of the or organic substance was little ,and this indicates that using the organic substance kolkhoz and clover led to a decrease in the effect inhibited to the pesticiot on the total number of microbe especially used in the case of uling a double close of pesticide used in the study . At the end of the experiment ,it was showed that the inhibitory effect, during a period of incubation and to days, was clearly least at using the organic substance with the three concentrations of pesticide. The result, also rescaled that the Nitrogen .and phosphorus in the soil reached the higest value after a period and (60)days and incubation in the treatment in the which the organic substance was used without wing the pesticide. But when the pesticide was used without the existence y the organic substance, the effect on the Nitrogen and phosphor was higher. The fixed effect was little especially at using the organic substance on the pesticide with all its three concentrations. whilst ,it is found that the organic carbon in the soil decreased gradually with the increase of the in cubation .Period. This is due to activity of the microbe in the soil

A Study of some Immune Factors in Patients with Bacterial Urinary Tract Infections Caused by Aerobic Bacteria.

Nabeel Ahmed Rajab, Neama Hamad Hassan --- Shiha

Journal of University of Anbar for Pure Science , volume:8 issue:3

One hundred and fifty seven (157) adult patients from both sexes were included in this study. Sixty(60) adult persons were presented as control groups . Patients were suffering from Urinary tract infec-tions.Urine and serum specimens were taken from each patient to be used for IgG , IgM , IgA and com-plement C3 study . Tamm Horsfall protein , IgM and IgG were studied in urine . Bacteriological investiga-tions were done for each urine specimen from patients and control groups. Sixty(60) patients were show-ing positive Urine culture , females were showing the highest ratio in all age groups particularly the first two groups(17-27) and (28-38) years old. Sera from patients of positive and negative Urine cultures were showing higher values of IgG, IgM, IgA and C3 than control . Higher values of THP were found in urine of patients with negative Urine culture and control groups . In conclusion,Tamm-Hors-fall protein,IgM, IgG and IgA are important to combat UTIs locally and systemic .

Immune and non-immune diagnosis of H. pylori In Patients with ingastric ulcer (GU) and non-ulcer dyspepsia (NUD).

Aseel k. Husain , Yasin H. Al-Fahdawi --- Sheha

Journal of University of Anbar for Pure Science , volume:8 issue:3

Background:The roles that T helper type 1 (Th1) specific immune responses in protection from H. pylori challenge was understood. It is expected that Th2 immune responses are required for protection against extracellular bacteria, such as H. pylori. Both invasive and non-invasive tests are used in the diagnosis of Helicobacter pylori infection. The non-invasive tests avoid endoscopy and encompass the serologic and breath tests This study aimed to show important immune and non-immune tests for Helicobacter pyloriinfection diagnosis in patients . Patients and Methods:A total of one hundred seven (107) adult patients from both genders were attending Gastro Endoscopy Unit at Ramadi Teaching Hospital to undergo selective OGD from December 2012 to May 2013. Multiple mucosal biopsy specimens were taken for rapid urease test (RUT) to detect Helicobacter pylori in tissue samples. After endoscopy, blood specimen was taken from each patient to be used for serological tests including; IgG, IgM, by ELISA. Rapid Chromatographic Immunoassay test (CAS) was used for IgG against H.pylori also . Results: Present study showed that the rate of infection in males was same as in females, and increased within age group (31-50) years old, it was found thathigher positive results of CAS, and RUT for H. pylori, especially in younger adults. Findings confirmed that a significant relationship between H. pylori rapid urease test (RUT) with IgG and IgM specific for H. pylori antigen.

Study the effect of foliar spraying with different levels of urea in some anatomical side for eggplant Solanum melongena L.

Sajid S. Saleem, Rassmy M. Hamad--- Hibba F. Abd

Journal of University of Anbar for Pure Science , volume:8 issue:3

The field experiment had been conducted in the Lath-house in the college of Agriculture , Anbar University by planting Solanum melongena L. Black beauty during agricultural season of 2010. Seedlings were distributed randomly according to complete randomized design (CRD) . The four concentrations of urea dissolved in distill water . The levels of urea were control (N0) ; 0.5 g/L(N1) ;1 g/L(N2);8 g/L(N3) . After 40 days of transplanting Eggplants were sprayed four times by urea concentrations . Anatomic study of cross-sections of root showed clear reduction in thickness of Epidermis, cortex and xylem tissues of plants treated with the concentration 8 gram urea/L where thickness of these tissues was 26.07 µm, 178.52 µm, 37.60 µm respectively. The phloem showed 224.32 um thickness. It was the highest among other treatments especially the treatment 1g urea/L which showed higher rate of xylem thickness of 373.68 µm. The cross-section of stem also showed clear difference in the epidermis thickness for all treated plants. In addition, 1gram urea/L showed higher cortex thickness of the stem,( 432.93 µm). Increasing urea concentration to 8 gram/L lead to reduce xylem thickness (104.72 µm) and decreasing pith thickness in the same treatment rated 426.67 µm. Plants treated with 1 gram urea/L, show increasing in the phloem and the pith thickness of 98.62 µm, 577.47µm rate respectively.The study showed that stoma distribution in the surface of leaves is of Anomocytic type with clear reduction of the outer epidermis thickness of 1gram urea/L treatment rated 16.05µm. This treatment showed highest rate in palasid tissues 98.40. The N2 treatment shows higher rate in stoma frequency on the upper and lower surfaces, 10 stoma/ml². The N3 treatment shows less number of stoma on the upper and lower surfaces, 6.04 stoma/ml². As for cell length in the two epidermis, the N2 treatment shows clear increase rated 59.08 in comparison with control treatment and othertreatments

Coated Wire and Multiwalled Carbon Nanotube Composite Coated Wire Sensors for Determination of Chlorpromazine Hydrochloride in Pharmaceutical Preparations.

Riyadh M. Jihad , Adawiya J. Hayder --- Ismael K.

Journal of University of Anbar for Pure Science , volume:8 issue:3

fabrication and performance characteristics of novel potentiometric sensors for the determination of chlorpromazine hydrochloride are described. The proposed sensors include a coated copper wire sensor and a multiwalled carbon nanotube (MWCNT) composite coated copper wire sensor. The sensors are based on the chlorpromazine-phosphotungstate ion associate as electroactive material. The developed sensors exhibited near nernstian slopes of 53.69 and 57.31 mV concentration decade-1 at 25 ?C, in the concentration range 5.0 x 10-5 – 1.0 x 10-2 mol L-1 chlorpromazine hydrochloride with limits of detection of 4.8 x 10-5 and 4.9 x 10-5 mol L-1 chlorpromazine hydrochloride for coated copper wire sensor and a (MWCNT) composite coated copper wire sensor, respectively. The proposed sensors exhibited good selectivity for chlorpromazine with respect to a large number of inorganic cations, amino acid and sugars. The developed sensor was successfully applied for the potentiometric determination of chlorpromazine hydrochloride in the pharmaceutical preparations and human urine samples.

Study of Seasonal variations of Main Outfall Drain and Four Northern Trocars in Iraq

Sufyan M. Shartooh --- Sidek A.Kasim

Journal of University of Anbar for Pure Science , volume:8 issue:3

Our study was aimed to assess seasonal variation of Main Outfall Drain and: Al-Es'haky, Sabe'e Al-Bor, Al-Saglawia and Al-Shuala trocars using several physical and chemical variables, water samples were collected from the surface about 10-15 cm in depth from these sites from February 2010 to January 2011. The results show that the mean of temperature was significantly (P ? 0.05) affected by seasonal temperature and seemed to be lowered during winter (13.26 ± 2.6 to 14.3 ± 1.9) and was high in summer ( 22.6 ± 2.6 to 23.9 ± 1.2 ) but un affected (P?0.05) by water collecting sites. For pH values, the obtained results seem to be almost not acidic and also not significantly influenced by either the climate seasons or by collection sites and found to range from 7.8 ± 0.2 to 8.8 ± 0.3. However, similar findings were recorded for electric conductivity where again insignificant impacts (P ? 0.05) of both climate seasons and collecting sites and mean values varied from 188.0 ± 0.0 to 2380.3 ± 10.0 µS/cm. Mean dissolved salt content was found to be significantly affected (P ? 0.05) by collecting sites but not by climate season and highest value (1260.0 ± 14.2 mg/l) was recorded in Al-Es'haky samples while the lowest value (1670.3 ± 30.0 mg/l) was in the Main Outfall Drain samples. However, the mean dissolved oxygen content was in general high in winter, spring and autumn and relatively lower in summer and such data were insignificantly differed (P ? 0.05) from each other in case of climate season but were very significant (P ? 0.05) in case of collecting sites. Al-Shuala samples had the lowest values (6.0 ± 0.4 to 8.0 ±0.2 mg/l) while Main Outfall Drain sample showed highest (8.4 ± 0.0 to 9.0 ± 0.1 mg/l) values. For oxygen saturated percentage, it was found that the mean value was not significantly (P ? 0.05) affected by both clime season and collecting sites except for Al-Shuala samples which was significantly (P ? 0.05) different. Similar findings were found for BOD mean values where the recorded data were again insignificantly (P ? 0.05) influenced by both climate season and collecting sites except Sbe'e Al-Bor trocar which had the significantly (P ? 0.05) lowest values ranging from 1.3 ± 0.3 mg/l in winter to 2.6 ± 0.3 mg/l in summer.

Analysis Of Iraqi Camels milk Components

Sami A.Mohammed Salih --- Mohammed Q. Al-Ani

Journal of University of Anbar for Pure Science , volume:8 issue:3

This study was performed in the laboratories of the Department of Biology Sciences ; Faculty of Science, University of Anbar and the Ministry of Science and Technology, and the aim of this study is to study the chemical properties and Physical for Iraqi camel milk was collected samples from 50 camels from the city of wetlands in western Iraq , was estimated by the chemical content Fat , lactose, ash , protein, and solids fatty solids SNF , and study characteristics Physicals and chemicals such as pH , acidity, specific weight , degree of humidity , and the degree of freezing , has been the study of fatty acids , and vitamins in the milk of camels, Iraq , and the results follows , the percentage of fat ( 2.45% ) and lactose ( 4.05 % ) , protein ( 2.75 % ) and solids College ( 12.31 ) , solid objects SNF ( 7.5 % ) , ash ( 0.65% ) , and the degree of pH ( 6.5 % ) , and pH ( 0.13% ) , and specific gravity ( 1.03 % ) , and humidity in camel milk 88.5 %) ) , and the degree of freezing ( 0.045 - % ) . The fatty acid content in Iraqi camel milk, and she was as follows saturated fatty acids Kaprlik - Cupric - Cabrk - Lorik - Mirstic - Balmtaic and unsaturated fatty acids , linoleic - oleic - linolenic , and the content of camel milk vitamins where he found that the percentage of vitamin C (3.0) 10 mcg / ml , and vitamin B3 niacin ( 2.68 ) 10 mcg / mL , and vitamin B12 (1.05) 10 mcg / mL , and vitamin B2 (0.51) 10 mcg / mL , and vitamin B6 (0.43) 10 mcg / mL , and vitamin B1 thymine ( 0.33) 10 mcg / ml , and acid folic ( 0.005) 10 mcg / ml .

Use specific immunoglobulin IgY purified from egg yolk and see its effect on the weights and lengths of the internal organs in chickens infected parasite Eimeria tenella

Thaer A. S. , Tawfiq A. AL-Aloosi --- Hajir A.A C

Journal of University of Anbar for Pure Science , volume:8 issue:3

Been obtained for the isolation of a local parasite Eimeria tenella by taking the cecca of 850 samples from slaughterhouses chickens in the city of Ramadi, and after purification and In vitro Sporolated diagnosed through the shape and size of the parasite and the infection, as has been confirmed diagnosis of the sample in the veterinary hospital / Ramadi , he received the strain challenge by strengthening the isolation and written consent of Chicken type Ross 308 five times, has been cracking the parasite by an ultrasound Sonicater and extract Sporocyst and Sporozoite in fourth Dose , injected Challenge Strain then in laying hens Type Luhman in different areas under the skin and muscle of the chest and thigh muscle and the muscle of the neck after mixing with the solution of Freund's incomplete oil adjuvant, was then the collection of eggs from the first day of the first dose until the end of the fourth dose of the control sequence to increase immunoglobulin IgY in egg yolk, extracted immunoglobulin IgY from egg yolk on a daily basis and has Dialysis and the deportation of protein extract crude by Vertical Electrophoresis with some standard protein, and measuring the concentration of crude protein by means of a spectroscope optical Spectrophotometer at wavelength 540, depending on Baurit it reached its highest level 137.12604 mg/egg , were separated IgY using Technology Column Chromatography packed gel Sephacryl S300 HR 95 cm high and 1.6 cm diameter and Determenation of molecular weight by separating the dye Blue Dextran 2000 and seven proteins that record different molecular weights and the work of the curve index, were also measured concentration of IgY extracted after separation column by Spectrophotometer it reached its highest level 117.61764 mg/egg, was deported IgY by Electrophoresis with standard IgG for the detection of a package Kama Globulin , conducted revealed clumping of IgY with strain fierce by the use of Single radial immunodiffusion test and Double immunodiffusion Ouchterlony test to determine the extent of specialization against the type of isolation and extraction of focus appropriate for use as a treatment in subsequent experiments, you saved the sample IgY by freezing degree zero -4 C The results showed significant differences between the treatments especially in infection region ( ceacum ) and that the best way to cure by giving injection therapy in the peritoneum and egg yolk inside body compared with negative control and the rest of the transactions .

Study of Adenosine Deaminase(ADA)Enzyme Activity of Children Patients With Kala-azar in Ameria City/AL-Anbar Governorate

Ban M.Abbas , Muthanna M.Awad --- Wajeeh Y.AL-Ani

Journal of University of Anbar for Pure Science , volume:8 issue:3

A study of the Activity Adenosine Deaminase of the infected children with kala-azar in ameria City/AL-Anbar Governorate, this study is conducted on a sample of 90 male and female children (the range of them between 3 months to 8 years) in the General Ameria hospital and healthy center in AL-Ameria (the period between November 2011 to the end of May 2012) distributed in two groups: the first group includes those who are kala-azar patients (60 subjects ) while the second one includes the control group (30 subjects) , then both groups are subdivided according to four types concerning their age: the first one is less than one year; the second ranges from 1 to 2 years; the third ranges from 3 to 4 years; the fourth includes those who are more than five years. The findings of the study show a significant increase in the activity of (ADA) in the serum of the infected children compared to control group at the probability level (p < 0.05) concerning effect of sex variable on the activity of (ADA), the study shows no significant difference between male and female infected children at the probability level (p < 0.05). As regarded age variable on the activity of (ADA), the study shows significant difference between the different types of infected children at probability level (p < 0.05). It also shows positive correlation relationship between (ADA) Enzyme and weight of the infected children at the probability level (p < 0.05 ) .

Adsorption of para - Chlorophenol on Activated Carbon of Drinking Water Purification Filters

Ayad Abdulrazzaq Mutar --- Majid Hadi Dhahir

Journal of University of Anbar for Pure Science , volume:8 issue:3

This work involves the study of adsorption of p-chlorophenol (PCP) from aqueous solution on the activated carbon of filter used in drinking water purification units ,which prepared from coconut shells .The adsorption studied at different PCP concentrations and different temperature spectrophotometrically . The value of adsorbed quantity (Qe) was measured for each concentration at equilibrium (Ce) and the percentage of adsorbed quantity (Qe%) was also calculated . The results show that the percentage of adsorbed quantity increased as the percentage of adsorbed quantity and increased as the concentration of PCP increases until the equilibrium condition obtained and also the value of (Qe%) is directly proportional to temperature . The isotherm of Langmuir , Freundlich and Temkin were tested and found that the results fits Freundlich isotherm better than Langmuir isotherm , while Temkin isotherm fit at 333k compared with other isotherms . The thermodynamic parameters (?G , ?H and ?S) were also calculated and show that the adsorption is endothermic and spontaneous process and increased as the temperature increases , the entropy of adsorption was also increased with temperature

Preparation and characterization of a New polymers for retention water as a Chemical method for decreasing irrigation waters

Tariq A. mindeel , Tamara A.AL-Salmani--- Ibrahee

Journal of University of Anbar for Pure Science , volume:8 issue:3

Using hydrophilic monomers, four new polymers for water retention has been prepared as Super Absorption Polymers (SAP) of water, under nitrogen atmosphere, in a free radical polymerization route, using Ammonium per-sulfate (APS) as an initiator and at constant temperature 70C0 and on several types ( Composite, Copolymer, Grafting and Modified ). H1NMR, FT-IR Spectroscopy were using for products characterization, and Differential Scanning Calorimetry) DSC) for thermals stability study. Water absorption capacity for hydrogel was n448 to 3163wt / wt).

Preparation and characterization of Nano Natural Silica composite with polyester resin & polystyrene and study of Mechanical properties

Mohamed A. -- Tareq A. Mandeel

Journal of University of Anbar for Pure Science , volume:8 issue:3

For the preparation of composite materials environmentally friendly natural nano particle silica derived from rice husks has as strengthening of Iraqi natural materials in a ratio of (2, 4, 6, 8%) on the polyester resin body and polystyrene separately. Natural silica was identified using XRD and FTIR by measuring the proportion of SiO2. AFM device was also used to measure the granular size of the silica nano particles. And the results of the study consistent with theoretical studies.Results also showed mechanical models composite. That added nano-silica of 6% to polyester resin body results are increase the impact resistance values, hardness, and bending rigidity. As in the case of polystyrene composite, the ratio of 2% is the optimized percentage of strengthening, which lead to increased values of impact , bending and hardness. these study also showed ability of natural silica composite mechanical properties to gives resistance to stress conditions during use

“Synthesis and Characterization of same Imide Schiff’s bases Derived for Trimethoprim and their biological evaluation”

Mohammad F. Mesher , Jalal A. Abbas Alheety --- Yo

Journal of University of Anbar for Pure Science , volume:8 issue:3

Synthesis and characterization mono imides derivatives for trimethoprim, through reaction of the later with one mole of maleic and phthalic anhydrides, using the classical reflux methods in addition to the microwave technique in the presence of glacial acetic acid. Conversion of mono imides derivatives prepared in (1) above to the Schiff`s bases by the condensation with substituted aromatic aldehydes or ketones, using conventional method and microwave technique. So that imides-imines derivatives have been prepared. All the compounds prepared above have been characterized by using the physical constant by recording the malting points and using the spectroscopic methods such as UV-Visible, FT-IR and H1NMR spectroscopy. Study the biological activity of some selected new compounds as anti-bacterial against four types of bacteria (Pseudomona aruginons , Escherichia coli , Staphylococcus aureus and Enterococcus faecalis) isolates by Biomerixe co. using the disk diffusion method. This study showed high biological activity of most prepared compound against the studying bacteria.

Studying the Radiation Contamination in the Soil and War Waste in the Falluja City Using the Solid State Nuclear Track Detectors (SSNTDs) CR-39.

Nabeil Ibrahim Fawaz --- Ahmed Mudhafar Ahmed

Journal of University of Anbar for Pure Science , volume:8 issue:3

In this paper practical study have been made to estimate the uranium (238U) concentration through the measurement of the radon gas (222Rn) concentration for different samples include soils and pieces of metals from different locations at AL-Falluja City which it was became one of the military operations, via detecting alpha particles that emits from radon gas using (SSNTDs) (CR-39). The maximum for the uranium and radon gas concentration has been found to be nearly { (0.0393 ppm) } and { (86.55 Bq/m3) } respectively, in al golan quarter. Minimum has been found in Al gameia quarter and estimate to be nearly { (0.0129 ppm) } and { ( 28.49 Bq/m3) } respectively. There is no radiation Contamination caused by uranium and its daughters in the Falluja City, so we may conclude that this city does not attacked with depleted Uranium bombs

A comparative study of coagulants used in water treatment

Ismail Kh. Jassem, Maher Kh. Ibrahim--- Saadi K.

Journal of University of Anbar for Pure Science , volume:8 issue:3

Turbidity is one of the of drinking water treatment problems. Turbid water contains molecules, plankton and colloids, is dealt with through the addition of coagulation chemicals in a process called coagulation and flocculation. In this research, three of the most commonly used coagulants used in water treatment process were examined for their efficiency in reducing turbidity. Those are Aluminum sulfate (alum), Ferric chloride and Poly aluminum chloride (PACl). Experiments were conducted using synthetically prepared turbid water by adding kaolin and using a Jar test instrument to define the optimum dosage from each chemical. The tests were conducted for two turbidity levels; 52 and 580 NTU. The results showed that Poly aluminum chloride is more efficient than both alum and ferric chloride. A dosage of 75 mg/l from PACl reduces the turbidity of the water from 580 to 4.55 NTU (99.2%), and from 52 to 1.5 NTU (97.1%). Applying the same operating conditions, Alum and FeCl3 should be a lower efficiency for turbidity removal. The efficiency of Alum and Ferric were 98.5% and 93.8% for water with 580 NTU, and 98.8% and 86.1% for water with 52 NTU.

Studying Some Mechanical Properties of Polyester Discontinuous fiber glassfilled by Al2o3 , Mg(OH)2 and SiC

Adil .S. Matuk

Journal of University of Anbar for Pure Science , volume:8 issue:3

In this studying Impact strength and hardness of polyester discontinues fiber glass composites filled by Al2O3, Mg(OH)2 and SiC filers of (5%Vol. and 15%Vol.).Composite fabricated by stander methods. The experimental results showed that composites that filled by these fillers improved their properties and the SiC composites exhibited biter Impact and hardness properties.