2013, volume:7 issue:3


Using DNA Sequencing to Detect and Verify Genetically Modified Maize (Zea mays L.) in Iraq.

Gaith L. Arif Ayoub O. Alfalahi

Journal of University of Anbar for Pure Science , volume:7 issue:3

Abstract: Polymerase chain reaction (PCR) was used to detect genetically modified maize. The results revealed that 10 out of 72 maize DNA samples were genetically modified. Verification of PCR ampliconse indicated that all GM maize were of MON810 type engineered with cryIAb gene under the control of the cauliflower mosaic virus P35S promoter and NOS terminator. Direct DNA sequencing for three out of the ten detected GM maize confirm the presence of P35S promoter and NOS terminator. Also, the constructed alignment of both genetic elements in comparison with their respective sequence in the NCBI database, indicated complete identity level for P35S, whereas nucleotide mismatches was detected in two sites of NOS118 terminator both on the forward strand. Meanwhile one site was corrected by the reverse strand, the other site may be resulted from Tag polymerase mismatches. These results approved the highly conservativeness of both detected elements and the completely absence of single nucleotide mutations. Results revealed clearly that nonauthorized GM maize was entered the national agriculture sector without authorities permission, which maximize risks of spreading GM maize all around the country and need to act immediately by setting strict legislation and monitoring system.

Determination of thyroid hormone levels in the serum of Non- dialyzed patients with chronic renal failure

May K. Ismael

Journal of University of Anbar for Pure Science , volume:7 issue:3

Abstract: Thyroid hormones, triiodothyronine (T3) and thyroxin (T4) play an important role in growth, development, and physiology of the kidney. The kidney has a central role in metabolism and clearance of these hormones as well as thyroid – stimulating hormone (TSH). Chronic renal failure (CRF) is a state of irreversible deceleration in renal function results in alterations in internal milieu, which affects the synthesis and secretory rate of hormones. To evaluate the thyroid hormone levels in non-dialyzed patients with chronic renal failure (CRF); 24 patients with CRF aged 30-70 years, mean±S.d. (48.458 ± 13.569) and 48 healthy volunteers who served as controls aged 30-70 years (43.104 ± 12.387) were studied for their thyroid function status using enzyme-linked immunosorbent assay(ELISA). The study showed significant increase (p<0.05) in the serum triiodothyronine (T3), thyroxin (T4) and thyroid stimulating hormone (TSH) levels in 25%, 20%, and 25% of CRF patients respectively as compared to the healthy groups; while 75% of CRF patients showed non significant low levels (p>0.05) for both T3 and TSH and 80% for T4. Also there was a high positive relation between TSH, T3, T4 levels respectively with both age and gender of chronic renal failure patients. The results suggest that patients with CRF have a state of thyroid dysfunction.

Comparative Study Between Aloe Vera (Aloe vera) oil And Lavenders (Lavandula Officinalis) Oil , with Three Type Of Ointment Floumizin In Burn Treatment in Rabbits.

Buthyna Abdul Hameed

Journal of University of Anbar for Pure Science , volume:7 issue:3

In the present study we used 8 Rabbit ,they divided four groups ,first group treatment by mixed herbal oils (alovera&lavender) after burning (second type),in back area. Other groups treatment by three floumizin ointment found in pharmacy that different manufacture . we observed that the mixture of Aloe Vera and lavender oil in specific ratio where much better in the treatment of burn ,when compared with other three groups of floumizin (different manufacture )were little action.

Saccharomyces boulardii as effective probiotic against Salmonella typhimurium in Mice

Shahlaa M. Salih* Manhal F.Ahmed* Abeer Y.Abdul-Ka

Journal of University of Anbar for Pure Science , volume:7 issue:3

Abstract:This study was designed to investigate the protective role of Sacchromyces bouladii on intestinal section of mice infected with Salmonella typhimurium. Mice were divided into four groups. Acontrol group is uninfected with bacteria represent (negative control) , a second group was infected with bacteria S.typhimurium 0.1 ml (2.5 ×107 cfu/ ml) only represent (positive control) , the third group Induced mice received oral dose of S. boulardii 0.1ml (1×109 cfu/mL). Treated mice received S. boulardii (1×109 cfu/mL) orally for 7 days, followed by Salmonella infection. At the end of the experimental period the histological Results showed that administration of S. typhimurium alone resulted in a necrosis, degenerative changes and inflammatory cells infiltration in intestinal sections as compared with normal section taken from uninfected mice, while pretreatment with the S. boulardii ameliorate this effect

POSSIBILITY OF USING SOME MICROORGANISMS TO CONTROL THE GROWTH OF Pythium spp.

SAJID SALAHUDDIN SALEEM BIHAR MOQDAD ABDULLAH

Journal of University of Anbar for Pure Science , volume:7 issue:3

The pathogenicity of three isolates of the genus Pythium (P.afertile , which was isolated from okra , hay isolate and cress isolate ) for many economical plants such as maize , sesame , cucumber and tomato were studied in artificially infested soils . These isolates were isolated from the roots of some infected plants and it's pathogenicity was determined according to it's ability to cause pre – and post emergence damping – off of seedlings. The pathogenicity tests showed that the P. afertile isolate recorded the maximum reduction in percentage of pre – emergence damping – off , where it's percent was 39.99% , followed by cress isolate as 32.08 % and hay isolate as 27.08% , whereas the cress isolate gave a significant reduction in percentage of post – emergence damping – off which was 27.35 % , followed by P. afertile and hay isolates which were 21.44 % and 16.35 % respectively .The antagonistic activity between the fungi that isolated from the rhizosphere of tomato and sesame plants and both Pythium's isolates (P. afertile and cress isolates) was studied on P.S.A. culture medium . The results of fungi isolated from the rhizosphere of tomato and sesame plants showed the presence of 13 different types of fungi in addition to Pythium's isolates which are belong to the following genera : Alternaria , Aspergillus , Cladosporium , Penicillium , Phytophthora , Rhizoctonia , Trichoderma , Verticillium and Fusarium . The microbial analysis showed the presence of all isolated fungi that mentioned above except the fungus Fusarium in the rhizosphere of tomato plant and both of the fungi A. wentii and Cladosporium in the rhizosphere of sesame plant. Results of this research demonstrated convergence in the response of antagonistic fungi against both Pythium isolates except the fungus Trichoderma , which was the most influential with inhibition rate reached to 66.37 % because of it's ability to mycoparasite pathogenic fungi

Prevalence of Fasciola gigantica among sheep in Al-Anbar govrnorate and study of effect for aqoues extract of Urtica urens L. on Viability of Parasite.

SUAD SH. SHAHATHA

Journal of University of Anbar for Pure Science , volume:7 issue:3

This study was conducted to investigate Prevalence of Fasciola gigantica in Al-Anbar govrnorate by test of (540) slaughtered sheep with different ages and sex (233 males and 307 females) in different areas in Al-Anbar govrnorate (Ramadi, Faluja, Heet, Hadytha, Rutba) by optical test for year 2012.The total infection was 8.3% The infestation percent in females was 10.4% which was higher than the infestation percent in males which was 5.5% .The infestation percent had increased with age progresses. The highest infestation percent was 14.2% at age more than eight years, Also, highest percentage of infection in Ramadi 11.3% and lowest percentage in Rutba 3.7%. Highest percentage of infecdtion was found in October 20% and November 13.6%which decline in June 3.2%.Significant differences were found between the number of Parasite in the gall bladder and their Liver for both males and females at (P> 0.05).The study included the inhibition effect of Urtica urens aqoues extract on Fasciola gigantica viability, the results indicated that F. gigantica viability decreased by (0.1) to (10) Mg/ml concentration and more decreasing by more concentrations, that the viability rate decreased

MAXED OF THE TYPES OF VEGETABLE OILS AND FILLED WITH DIFFERENT BAGS ON LIFE SOUTH COWPEA BEETLE Callosobruchus maculates ( Fab .)

HUSHAM NAJI HAMMED

Journal of University of Anbar for Pure Science , volume:7 issue:3

The study was conducted to study the effect of many kinds of Legume grain treated with plant oil on the biology and development of Callosobruchus maculates ( Fab.),those Legume were White and red cowpeas , chick-pea fine and coarse, coarse soft Legume. They were mixed with three kind of plant oils with mixing rate of 5 % for all kind of plant oils, Oils used in this study were: olive oil, castor oil with two period s of storage (2) and (4) months, and sunflower oil with period of storage(2) months. Seeds were packaged In three types of bags : jute bag, Cloth and pvc , and then the all seeds bag were infected with five pairs of adult insect newly emerged. The results showed: That the type of oil and the storage period and the type of bag significant effect in protecting seeds from injury C. maculates and top protection for grain legume treatment Oils in the castor oil followed by olive oil and less sun flower oil, chickpeas coarse grains and beans fine and coarse less affected "and a preference in the nutrition of the insect, and the type of bag was the best protection in a cloth bag and followed a jute bag and bag pvc , And that the storage period of two Months better than four of the lack of grain as well as the lack of injury weighing in consumption due to the low productivity of the insect

DIAGNOSIS OF ISOLATED BACTERIA FROM PATIANTS OF TONSILLITIS IN RAMADI AND IDENTIFICATION OF SENSITIVITY TO SOME ANTIBIOTICS

SAAD . S . H . ALI HANAA . A. YASIN

Journal of University of Anbar for Pure Science , volume:7 issue:3

Total oral bacterial samples ( 122 ) were collected form several (E-N-T) units in Educated Ramadi hospital in the period between the first of October to the end of March 2013 isolated including 211 isolates of bacteria were obtained 8 types of bacteria in order to isolate and identify the types of bacteria that can live with tonsillitisand this study included both sexes with the age range of (1 – 46 and up) years. Many gram negative and positive bacteria were isolated and the highest percent 19.4 % (41 Isolates)was related to the type Moraxella catarrhalis while the lowest percent 4.2 % (9 Isolates) was related to Klebseilla pnumoniae .However, the rest isolates were recorded to be 17.6%(37 Isolates) for Staphylococcus aureus., 14.7 % (31 Isolates) for Streptococcus pyogenes .,13.8% (29 Isolates)for Haemophilus influenzae ., 12.3%(26 Isolates) for Streptococcus pneumoniae., 9.5%(20 Isolates) for Staphylococcus epidermids and 8.5%(18 Isolates) for Streptococcus viridians. This study showed that there was no significant effects of the sex and age but there was significant effects of the place of living on the infection.Bacterial isolates showed high sensitivity to Gentamicin, Imipenem and the rate was 100% sensitivity.Gram negative bacteria showed resistance to antibiotics 11.11%of bacteria isolates were resistant to Amikacin while 33.33% of them were resistant to Cefotaxime and Tetracycline and 44.44%of isolates were resistant to Erythromycin and 66.66% of isolates were resistant to Clindamycin and Azetreonam and 77.77% of isolates were resistant to Vancomycin while all isolates of isolates were resistant to Amoxycilin / clavulanic acid. As shown gram positive bacterial isolates to were less resistant to antibiotics uses, the ratios as follows: Cefotaxime , Erythromycin and Azetreonam %6.66 Clindamycin 20 % and Vancomycin and Amoxycilin /clavulanic acid 40% While all of gram positive isolates were sensitive to Tetracycline

DIAGNOSIS OF ISOLATED BACTERIA FROM PATIANTS OF TONSILLITIS IN RAMADI AND IDENTIFICATION OF SENSITIVITY TO SOME ANTIBIOTICS

SAAD . S . H . ALI HANAA . A. YASIN

Journal of University of Anbar for Pure Science , volume:7 issue:3

Total oral bacterial samples ( 122 ) were collected form several (E-N-T) units in Educated Ramadi hospital in the period between the first of October to the end of March 2013 isolated including 211 isolates of bacteria were obtained 8 types of bacteria in order to isolate and identify the types of bacteria that can live with tonsillitisand this study included both sexes with the age range of (1 – 46 and up) years. Many gram negative and positive bacteria were isolated and the highest percent 19.4 % (41 Isolates)was related to the type Moraxella catarrhalis while the lowest percent 4.2 % (9 Isolates) was related to Klebseilla pnumoniae .However, the rest isolates were recorded to be 17.6%(37 Isolates) for Staphylococcus aureus., 14.7 % (31 Isolates) for Streptococcus pyogenes .,13.8% (29 Isolates)for Haemophilus influenzae ., 12.3%(26 Isolates) for Streptococcus pneumoniae., 9.5%(20 Isolates) for Staphylococcus epidermids and 8.5%(18 Isolates) for Streptococcus viridians. This study showed that there was no significant effects of the sex and age but there was significant effects of the place of living on the infection.Bacterial isolates showed high sensitivity to Gentamicin, Imipenem and the rate was 100% sensitivity.Gram negative bacteria showed resistance to antibiotics 11.11%of bacteria isolates were resistant to Amikacin while 33.33% of them were resistant to Cefotaxime and Tetracycline and 44.44%of isolates were resistant to Erythromycin and 66.66% of isolates were resistant to Clindamycin and Azetreonam and 77.77% of isolates were resistant to Vancomycin while all isolates of isolates were resistant to Amoxycilin / clavulanic acid. As shown gram positive bacterial isolates to were less resistant to antibiotics uses, the ratios as follows: Cefotaxime , Erythromycin and Azetreonam %6.66 Clindamycin 20 % and Vancomycin and Amoxycilin /clavulanic acid 40% While all of gram positive isolates were sensitive to Tetracycline

DETECTION OF HEPATITIS B VIRUS USING ELISA AND REAL TIME PCR TECHNIQUES IN PATIENTS BLOOD AND SALIVA IN AL- ANBAR PROVINCE

THAER A. HASAN AMIN S. BADAWY

Journal of University of Anbar for Pure Science , volume:7 issue:3

:The present study has been carried out at the laboratories of the department of biology at the college of Education-University of Tikrit for the period from 1st Oct to 1st May 2012. The study consisted of the investigation about Hepatitis B in the saliva as compared with serum by using the ELISA and Real Time PCR techniques. Patients fallen asleep and revisers of the Ramadi General Hospital and the Fallujah General Hospital and who had confirmed cases hepatitis B for the purpose of pattern prove of saliva in the portability infection, where the results of the study were as follows: The results obtained showed that HBsAg antigen was found with 99.4% and (1.101± 0.335) mean ±SD in serum as compared with 48.6% in saliva with a (0.330±0.278) mean ±SD, that gave a noticeable increase when comparing with the control group ( 0.039 ± 0.024 ) and ( 0.017±0.014 ) for serum and saliva respectively. The value of T test for serum and control group equals( 16.25 ), while the value of T test for saliva and control group equals (4.18) which has high significance equals (0.000) with a test value equals ( P < 0.05). The proportion of correlation between saliva and serum in ELISA test equals (0.361) with high significance of test while the value of T test for them equals (28.902) with high significance of test with a test value equals ( P < 0.05). The genetic factor has been used in order to determine the levels of HBV DNA in the samples of positive saliva to test ELISA by using Real Time PCR . 95% from tested serum samples were positive to the viral load. While in saliva 50% from the samples HBV DNA was positive

EFFECT OF COLD AND BOILED WATER EXTRACTS OF MELIA AZEDARACH AND MENTHA PIPERITA ON ADULTS MORTALITY OF TROGODERMA GRANARIUM

FOUAD A.ABDULLHA

Journal of University of Anbar for Pure Science , volume:7 issue:3

Efficacy of different concentration ( 1, 3, 5, % ) of cold and boiled water extracts of Melia azedarach and Mentha piperita on mortality of adults of khpra beetle Trogoderma granarium was studied .Daily insects mortality percentages and accumulative mortality after 72 hours of treatment with aqueous extracts was counted. The results showed that the highest accumulative mortality percentages of adults insect 86.6% and 83.3% recorded when larvae treated with 5% concentration of boiled water extract of Melia azedarach and cold water extract of Mentha piperita ,Respectively .Cold water extract of Mentha piperita in all concentration that used in this study caused high adults mortality percentages compared with low mortality percentages recorded when insect treated with boiled water extract , while adverse results showed in larval mortality percentages when insect treated with boiled and cold water extracts of Melia azedarach . The highest mortality percentages were recorded in larvae which treated with boiled or cold water extracts for both plants in the first day after treatment and these mortality percentages decreases gradually in the second and third days after treatment

STUDYING THE INHIBITION ACTIVITY OF CINNAMOMUMCASSIA CASSIA EXTRACTSON THE ORAL (MOUTH) BACTERIA

Journal of University of Anbar for Pure Science , volume:7 issue:3

Total oral bacterial samples ( 126 ) were collected form several dentist units distributed in various areas throughout Fallujah and Ramadi in order to isolate and identify the anaerobic types of bacteria that can live orally and that bacteria which cause a bad breath in human. The study included both sexes with the age range of 5 – 67 years. Many gram negative and positive bacteria were isolated and the highest percent 20.63 % was related to the type Streptococcus mutans while the lowest percent 6.34 % was related to B. subtilis. And for the first time in Iraq P.gingivalis was isolated with a percent of 7.9 % which found to be involved in the teeth infections.The sensitivity of these bacteria to nine antibiotics was tested. The highest inhibitory average reached a diameter 16.875 mm when using Chloramphenicol While Metronidazole gave the lowest inhibitory average 1.976 mm.The chemical test on the effective groups of Cinnamomum cassia was conducted and the results showed that the cortex contained most of the effective inhibitory groups of bacterial growth which make the plant to have its medical importance.These plants inhibitory substances in the cortex as extracts were studied on the bacterial isolates .The oil extract gave a superior significant effect than the others in inhibiting bacterial growth. Then after ethanol extract then cold water extract and finally hot water extract which had the lowest effect.The resulted inhibitory diameters upon using these four extracts were compared with the inhibitory effects of the antibiotics used, the highest inhibitory effect was reached by using the various plant cortex extracts except Staphylococcus spp. which was affected more by the antibiotics, Gentamicin and Rifampin than by the plant extracts

EFFECT OF ALUMINUM SULFATE ON SOME OF LIVER AND THE KIDNEY FUNCTIONS IN WHITE RAT Rattus norvegicus

Journal of University of Anbar for Pure Science , volume:7 issue:3

The present study concerned the effect of Aluminum Sulfate on some of liver and the kidney enzymes in white rat '' Rattus norvegicus '' . had used twelve rats for adult male and divided to four groups the concentration of Aluminum sulfate in first group was 75 mg 100 ml of distilled water the second was 150 mg 100 ml of distilled water & third 300 mg 100 ml of distilled water that’s gave as 1 ml per day ; the first group is considered as control group , while the other three groups are experimental groups . The study have found there are significant differences in ALP, ALT and AST enzymes in addition to Creatinine and Urea with dosage increase concentration of Aluminum sulfate by compare with control group (P?0.05) However , there are no significant differences in ALT enzyme when concentration of this dosage is increased among experimental groups ( 1,2,3 ) . Adding to that there are significant differences in AST enzyme when concentration of this dosage is increased in experimental groups 1 , 2 and 3 (P?0.05) while there are no significant differences in ALP and Urea when the concentration of the dosage is increased in comparison with experimental group 1,2,3 (P?0.05).Finally , they are significant differences in Creatinin when the concentration of the dosage is increased in the experimental groups in comparison with 1,2 and 3 (P?0.05 .The study shows that Aluminium sulfate effect negatively on the level and the activity of liver ALP ,ALT , and AST enzymes , Also , It has negative effect in the activity and the level of both creatinine and urea of kidneys whatever the concentration of the dosage in the water it was . In addition to that , there are differences in the effect of Aluminium sulfate on the ALP ,ALT , AST enzymes and creatinin and urea concerning levels of their activities inside Ruttus

STUDY OF ANTIMICROBIAL SUSCEPTIBILITY OF Escherichia coli OF ANTIBIOTICS ISOLATED FROM URINARY TRACT INFECTIONS OF CHILDREN AND DETECTION OF PLASMID DNA CONTENT IN BACTERIAL ISOLATES BY GEL ELECTROPHORESIS TECHNIQUE

Journal of University of Anbar for Pure Science , volume:7 issue:3

Twenty bacterial were isolated of Escherichia coli from children with urinary tracts infection. These isolates were identified by cultural and biochemical tests and Antibiotic susceptibility tests were carried out to determine their resistance patterns so they were 75%, 100%, 50%, 100%, 80%, 90%, 75%, 60%, 100% and 35% resistant to Ampicillin, Amoxicillin, Neomycin, Kanamycin, Streptomycin, Tetracycline, Nalidixic acid, Ciprofloxacin, Erythromycin, Norefloxacin respectively

STUDY THE RELATION BETWEEN SELENIUM AND GLUTATHIONE PEROXIDASE ACTIVITY IN IRON DEFICIENT GUINEA PIG.

Journal of University of Anbar for Pure Science , volume:7 issue:3

Little information is available on the relationship of Selenium deposit in target organs and Glutathione peroxidase (GPx) activity in iron deficiency anemia ( IDA). As red blood cells (RBCs) play a crucial role on Se metabolism during Fe deficiency anemia a lower count of RBCs is featured, we aimed to investigate the influence of this pathology on Se bioavailability and the relationship with antioxidant status. 20 male guinea pig were randomly divided into two groups, a control group receiving 100 g diet with normal Fe content (45 mg/kg diet) and the Fe-deficient group receiving 100 g diet with low Fe content (5 mg/kg diet) for 60 days. Both diets were prepared with an adequate Se content (0.180 mg/kg diet). The digestive and metabolic utilization of Se, the distribution in target organ, the GPx activity and thiobarbituric acid-reactive substances (TBARS) production were measured after receiving the diets. Se retention increased (P ? 0.001) in the anemic group, fact that contributes to keep the enzymatic antioxidant activity of GPx in normal levels and the tendency observed is that stored Se increased in the organs, especially in kidney (P ? 0.01) . The lower count of RBCs featured in this pathology (P ? 0.001) causes an increase in kidney deposit is a consequence of the lower urinary losses (P ? 0.001

EXTRACTION AND SEPARATION OF SOME ACTIVE COMPOUNDS IN ERUCA SATIVA AND STIUDY ITS BIOLOGICAL ACTIVITY

Journal of University of Anbar for Pure Science , volume:7 issue:3

This study aimed to sperat and extract of some active compounds in Eruca sativa .It included identificaion and determination of active compounds their percentages in the leaves,stem and seeds,like(Tannine, Glycosides,Alkaloids,Saponins,Resins,Flavonoids and Terpens). The results obtained were showen that these compounds are found in all part of the plant .The stem is not contain Terpenes and Resins .The highest percentages for active compounds was in leaves like the percentages .For the Tannins and Glycosids and Alkaloids and Saponins,these were 14.3%,6%,11%,7.4% respectively. For basic found chemical compounds such as (protein,carbohydrates,total oils and ash) in the main parts of plant . It was found that the highest percentages of protein and carbohydratesre were in leaves at 36%,28% respectively .While the total oil percentages was 29% in seeds . The highest value of percentages for the moisture was 9.4%. Determined of the major mineral like (Ca,Na,K,Li,Ba) in the main part of plant using flame photometer technique was refereed that calcium present of higest concentration in the main parts of plant.The study has been performed of activity bilogical inhibited for extacts prepared from leaves towards yeast Candida albican and fungi Geotrichum candidum using well indiffusion agar.This happened by use of different concentration (10-100)mg/ml and use solvents(water,methanol and ethanol).The study explaind that were significant differeces between the aqueous extract which has been least inhibited activity towards fungi and the ethanolic extract which gave highest activity against yeast Candida albicans .The inhibition zone was (17.3)mm

STUDY THE EFFECT OF CONTRACEPTIVES ON THE LEVEL OF THE TOTAL ANTIOXIDANT CAPACITY, VITAMIN C AND IRON IN THE BLOOD SERUM OF WOMEN

Journal of University of Anbar for Pure Science , volume:7 issue:3

The use of contraception in all its forms (hormonal, mechanical) is spreading widely in all societies, This study was conducted to determine the effect of these barriers especially hormonal in reducing the effectiveness of some types of antioxidants. the current study was conducted in the Fallujah General Hospital , as study included 68 women using contraceptives (26 have used Oral Contraceptive Pills (OCP), 29 who used Intrauterine Device (IUD), 13 who used Injection progesterone depot medroxyprogesterone acetate (DMPA), average age (35 years) and 16 women in the control group the average age (33)years. been collecting blood samples from all groups for the purpose of assessing the effectiveness of some antioxidants found in serum and include the overall capacity of antioxidant (TAC), vitamin C and iron Fe element. showed the results of the current study: • The Conc.of vitamin C significantly decreased (P <0.05) in the group of women who have used (OCP) (0.77mg/dl), in the group of women who used(IUD) (0.90mg/dl), and in women who have used(DMPA)(0.97 mg/dl) when compared with control group (1.38mg/dl). • The levels of total antioxidant of capacity (TAC) significantly decreased (P <0.05) in the group of women who have used(OCP)(1.06 mmol/L) and in women who have used(IUD )(1.06 mmol/L) and in women who have used (DMPA)(0.78 mmol/L) when compared with control group (mmol/L1.62). • The Conc.of Iron significantly increased(P<0.05) in women who have used(OCP)( 3.25mg/L)and women have used(IUD) (2.88mg/L) and women have used(DMPA)( 4.03mg/L) when compared them all with the control group(1.28mg/L).

INDUCED PHOTO-DEGRADATION OF POLY (VINYL CHLORIDE) BY USING COBALT (II) CHLORIDE.

Journal of University of Anbar for Pure Science , volume:7 issue:3

: This research has been studied the photodegradation for poly( vinyl Chloride) when expose it to the light rays as it mixed with cobalt chloride duo (CoCl2.6H2O), polymer films had been prepared after mixing them with polymer solution by proportions grains ranged between (0.025% - 0.4%) and thickness was around (70 ± 5) microns in tetrahydrofuran (THF) as a solvent. Specimens were observing degradation of polymer films by using light intensity (1.5x10-8 ein.dm3s-1) at wavelength (356nm) at 40?C at different radiation times. This was occurring in the absence and presence OF Cobalt (II) Chloride by using Fourier trans form infrared spectroscopy (FTIR). During the observation of the growth of the carbonyl Index (ICO) and hydroxyl (IOH), as well as using spectroscopy UV - visible ( UV), as Speed optical dissociation constant of polymeric additives, the results has showed that when the concentration of Cobalt (II) Chloride increased, the degradation of polymer films increased compare to free of salt polymer films. As this accompanied increased in the values of absorption of carbonyl and hydroxyl. It has been noticed that when this increase was a little bit higher when the concentration around 0.4% , which these results have an agreement with optical speed dissociation constant. Moreover, at the absence and presence ofh Moreover, at the absence and presence of concentration (0.05%) polymer film’s photodegradation was studied from salt added as observing the average viscosity of molecular weight, degree of degradation which found out Cobalt (II) Chloride decreased in the molecular weight as the rate chain increased. Finally, polymer films that had been prepared by morphology was studied in the absence and presence of the salt. This study was investigated to find out the amount of energy absorbed by those films and the possibility of cracks in the surface after exposing of those models to irradiation and time of 125 hours

PHYSIOLOGICAL AND BIOCHEMICAL STUDY FOR SPECIMEN OF CHILDREN SUFFERING OF ENZYME G6PD DEFICIENCY AT RAMADY CITY.

Journal of University of Anbar for Pure Science , volume:7 issue:3

Some blood variable and reduced Glutathione (GSH) concentration had been studied besides lipid peroxides (Malondialdehyde (MDA)) and the concentration of trace ions of the element (Fe+2 , Cu+2 , Zn+2) in the blood serum of ill mace children. The study including sixty children suffering of acute hemolytic anemia of ages between (1-10)years ,more over another 30 healthy childs appearance of the some ages as a control group .The result indicated significant decrease at (P ? 0.05) of the activity of the enzyme G6PD in the children suffering illness due to the enzymatic deficiency in comparison with healthy children where the value of the enzyme activity recorded illustrated that the suffered children recorded (1.77 ± 0.71) U/g.Hb while the healthy children was (12.64 ± 2.40) U/g.Hb . The result also showed the children suffering G6PD lack recorded low significant result (P ? 0.05) at both the concentration of hemoglobin , site of packed red blood cells , reduced Glutathione and the concentration of Zn+2 relatively to healthy, where its concentration for the suffering children (5.82 ± 1.16) g/dl , (18.43 ± 3.81) % , (2.95 ± 0.99) µmol/l and (15.78 ± 4.23) µmol/l while for the healthy were (11.95 ± 0.67) g/dl , (37.76 ± 1.98) % , (7.80 ± 2.20) µmol/l , (20.60±3.06) µmol/l respectively. While it revealed significant elevation (P ? 0.05) at both white blood cells and the MDA concentration ,besides the concentration of Fe+2 and Cu+2 at the Infected children regarding the healthy children. Where by its concentration at Infected children (8.46 ± 2.03)*103 cell/mm3 , (20.83 ± 7.75) µmol/l , (81.75 ± 48.58) µmol/l and (44.29 ± 10.88) µmol/l. while for healthy were (16.14 ± 5.57)*103 cell/mm3 , (8.98 ± 2.47) µmol/l , (33.48 ± 5.23) µmol/l and (33.19 ± 6.76) µmol/l.

EFFECT OF BOILING TIME ON EXTRACTED TANNINS FROM TEA LEAFS

Journal of University of Anbar for Pure Science , volume:7 issue:3

ABSTRACT : Extraction of tannic acid from black leaf Ceylon tea was studied as a function of boiling time (5-30 minute) at 100 0C. Results indicate clearly, that the percentage yield of extracted tannic acid increases with increasing boiling time. About 48% of tannic acid was extracted from tea leafs for 30 minutes boiling.

MAGNETIC FIELD BASELINE DETERMINATION FOR BAGHDAD CITY

Journal of University of Anbar for Pure Science , volume:7 issue:3

This research is conducted in Baghdad magnetic observatory – Jadriya by monitoring the measured total magnetic field around the clock for a whole year, for the purpose of defining magnetic baseline in order to identify the actual magnetic background for the site of Baghdad magnetic observatory. These observations show daily and seasonal variations in the field according to weather conditions and other factors in upper atmosphere. These variations are reflected directly on the earth magnetic field. Therefore, these readings have been plotted and analyzed to determine the magnetic baseline for the Baghdad observatory site. The average value was found to be equal to (45473.08) Gamma. It was shown that there is a unique value for each month of the year, that varies slightly according to sun activities during the summer.

EVALUATION AND ENHANCEMENT OF CONTRACT IN DIGITAL IMAGES

Journal of University of Anbar for Pure Science , volume:7 issue:3

The optical imaging systems, do not give a perfect and ideal image. But mostly give images with limited resolutions i.e. give images with blurr edges. This blurring attributed to two main reasons. The diffraction of light and Aberrations of the optical imaging system. The most important information and details in the image that contained in image edges. The fidelity criteria are evaluate the quality, depending on the whole image plane or in homogenous image regions. So that we devoted in this study to evaluate image quality depending on compute the image contrast in edge regions, and introduce robust quantitative measures to determine image quality, then estimate the efficiency of the various techniques in image processing applications. In this study we suggested new techniques to calculate image contrast (visibility) and studying it as a function of number of smoothing iterations from using mean filter and a function of gray level resolution. We only study the contrast in edge regions where we used Soble operator to find image edges. The suggested techniques are:- 1- Technique of measure contrast based on calculates the mean of dark points and the mean of bright points in the image edge regions. 2- The technique compute the triple contrast of the image where the first: global contrast (C0) in image edge compute as in direct technique, the second local edge contrast compute for the largest image edge points, while the third is the local contrast for the smallest image edge points. The results give high agreement among the various suggested methodsin determines image contrast. As we can theoretical guested, that the contrast reduced with reducing spatial and image gray level resolutions.Here can be say that we get a robust quantitative measures that could be used to estimate the efficiency of the image processing techniques, based on determine image contrast and find the amount of variation in contrast that causes from the processing steps.

Application of concepts of graph theory to determine the difference between some cyclic organic chemical compounds.

Ali R. Ibrahim

Journal of University of Anbar for Pure Science , volume:7 issue:3

In this paper, we use the concept of paths in graph theory in order to distinguish some cyclic aliphatic organic chemical compounds (13 compounds) and determine the difference among them. By increasing the length of the path from (p0) to p4 (p0?p4) divided into four cases, we which to study the nature of internal structural of the compound according to the length of each case and identify the complete structural spectrum (CSS) for each compound. Then we find the index of complexity of these compounds to find the number of classes for the set of compounds, where the increase in the number of classes leads to increase the accuracy of distinguish, which we got in the fourth case because the number of classes equals the number of elements of the set of compounds so that for each compound in this case, the properties differ from other compounds. Thus, increasing the length of the path means an increase in the description of the nature of the internal structural of the compound and we also wish to determine additional properties for each compound from which to distinguish it compared with the rest of the compounds.