Wrood Mutleb Farhan,; Hanaa Abd AL-latef Yaseen
Abstract
The study included 250 vaginal swabs were collected from women patients clinical symptoms of bacterial vaginitis and they have come to the women's and children's hospital in Ramadi ...
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The study included 250 vaginal swabs were collected from women patients clinical symptoms of bacterial vaginitis and they have come to the women's and children's hospital in Ramadi and ages (17- 45 years) nearly, to Investigation of the bacterial species positive and negative gram stain were Infected of the vagina, and also To determination the appearance and molecularly of virulence factors. The vaginal swabs were cultured, 86 samples were found to give positive growth of 34.4%, but (164 sample) 65.6% were negative. The growing isolates were subjected to bacteriological and biochemical tests. The isolates of positive gram stain 48 about 55.81% were having character of Staphylococcus epidermidis 18 about 20.93% and Staphylococcus aureus (9 (10.46%), Streptococcus pneumoniae 7 nearly 8.13%, Staphylococcus saprophyticus 6 about 7.97%, Streptococcus pyogenes 4, (4.65%) and Bacillus subtilis only 3, (3.48%) and Streptococcus viridans was only 1 isolate about 1.16% . The isolates of negative gram stain were recorded 38 percentage 44.18% . The results of the tests a bacteriological and biochemical were recorded Escherichia coli 15 isolates percent 17.44% and 14 of Klebsiella pneumoniae 16.27% , Pseudomonas aeruginosa was 6 percent 7.97%, Gardnerella vaginalis 2 about 2.32% , and Proteus mirabilis was one isolate only percent 1.16% . The polymerase chain reaction (PCR) technique were proved, the exoA and exoS genes were encodes toxin production. algD was encoded the production of the capsule which were found in Pseudomonas aeruginosa percent 100% for the three genes. In Staphylococcus aureus found that (hla) gen that encodes production of alpha-hemolycin 66.66% and finbA which encodes the production of (adhesion protein) 100%, as well as investigate some of the virulent factors of the vaginal bacteria, which included their ability to produce the enzyme hemolysis, urease and protease.