Muthana M. Awad; Inaam N. Ali; Alaa Sh. Mahmood
Abstract
The current study included understanding the effect of methotrexate and omega-3 in the thyroid and parathyroid gland and some of the physiological and histological and genetic variables ...
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The current study included understanding the effect of methotrexate and omega-3 in the thyroid and parathyroid gland and some of the physiological and histological and genetic variables in some of the organs of the body in the male rats. The study included 56 animals were divided into three groups, the first and second groups were divided into three secondary groups, the first secondary group was given the drug methotrexate only in concentration 0.05 , 0.125 and 0.250 mg / kg for 8 weeks while the secondary second group was given drug methotrexate in the same concentrations of the first group, as well as give it omega-3 concentration of 300 mg / kg for 8 weeks, the third group was considered as control group. After finishing of dosage, the animals killed and took blood samples and organs to do the required tests, included measuring of blood parameters, such as RBC ,PCV ,Hb ,MCV, MCH and MCHC. In addition to measure the number of white blood cell count WBC and Differential white blood cells, where the results showed the presence of a rise in both the RBC, PCV, Hb in the low and medium concentration of methotrexate while decreased in high concentration, omega-3 work to reduce the variables above in the low and medium concentration while worked on reducing it in the high concentration, moreover a variable of MCV and MCH and MCHC has not changed morally. With respect to the WBC was low in the totals for the study when giving methotrexate while rose when giving omega-3 with this druge, also neutrophil cells was low and accompanied by a rise in a monoytes in all concentrations of Methotrexate were not for omega 3 noticeable effect on these cells decreased. The results showed a decline in the lymph and acidophilus cells in the low concentration while rose in the medium and high concentration of Methotrexate. Omega-3 worked on the rise it in concentrations above, with respect to basophiles cells increased in the low and high concentration and decreased in medium and the omega-3 was not effect of them.
