Tamadir A. Rafee; Rasheed M. Rasheed n; Ahmed A. Sulaiman; Safaa K. Al-Amee
Abstract
This study was conducted to measure a group of hormones related with infected individuals with the shortage of the growth hormone by studying 150 patients in comparison with 50 samples ...
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This study was conducted to measure a group of hormones related with infected individuals with the shortage of the growth hormone by studying 150 patients in comparison with 50 samples taken from normal individuals as a control group. The study included investigation GH gene via polymerase chain reaction technology (PCR) by two primers designed specifically for this study (GH3, GH4). The results showed that mutations occurred in 12 individuals from the samples, 8% for GH3 , whereas the GH4 showed no absence or disorder in the sequence of bases. The results of Sequencing using the Blast program showed appearance of many mutations of deletion mutations and insertion mutations , and substitution mutations of two types of transition and transversion. The concentration of hormones has been measured were human Testosterone hormone , Prolactin hormone ,Cortisol hormone ,The results showed a highly significant differences in the levels of the averages of hormonal analysis , as well as a significant decrease in the average length of individuals and high moral appeared in body mass index(BMI) under the moral under the moral level of P ≤ 0.05.
