Sarab F. Al-Ani; Mohamed T. Dagash; Mahmood Jasem Al-Shabani
Abstract
Forty specimens of surgically removed hydatid cyst were obtained from patients in different hospitals in Al-Anbar province. The aim of study was designed to determine the type of common ...
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Forty specimens of surgically removed hydatid cyst were obtained from patients in different hospitals in Al-Anbar province. The aim of study was designed to determine the type of common larval stage (hydatid cyst) of echinococosis in Al-Anbar province according to PCR molecular differentiation.The study was carried out during the period from first day of January to the last day of December 2013.The age of patient ranged from (10 – ≥ 65 ) years old, (19 males and 21 females). A questionnaire paper was performed to each patient which includes information about age, sex,residence,occupation,exposure variable, water source, site of hydatid cyst and case history.From the obtained results, it's revealed that the age group (21 – 30 years) presented high hydatidosis parasitic infestation which was 35% , whereas the age group more than50 years presented low parasitic infection which was 2.5% . 47.5% of patients were males and 52.5% were females.Twenty eight(70%) patients live in rural areas. Also, twenty patients (50%) were exposured to livestock animals, (40%) of patients were farmers, twenty seven patients(68%) were consumed river water, The most common localization was the liver (65%). Purification of DNA was done by QIAamp DNA Mini kit from QIAGEN for cyst. Specific primers were designed for the diagnostic PCR. It amplifies a 133-basepair segment within the newly described repeated unit that amplified a target repeated sequence (EgG1 Hae III) newly identified in the genome of the common sheep strain of E. granulosus. Primer based on the flanking sequences allowed the PCR amplification of E. multilocularis, a fragment of mitochondrial 12S rDNA was amplified by PCR from human genomic DNA by using the cestode-specific primers 60 were used as targets for (PCR) amplification.The total number of the cysts specimens that gave positive results of E.granulosus by PCR was 37 (92.5%) patients and the number of specimens that gave negative results was 3(7.5%),while all specimens gave negative result of E. multilocularis by using PCR analysis.The present study showed that E. granulosus is a major causative of hydatidosis in Al-Anbar Province while E. multilocularis is not detected by using PCR approach.
