Inaam N. Ali; Hussein A. Sabtei; Khalid F.Hassan; Ganiya A.Hamdan
Abstract
In this study, essential oil of plant Allium sativum (garlic) which belongs, to the Liliaceae family was used at concentration 0, 1%, 5% and 10% to know their effect on the growth of ...
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In this study, essential oil of plant Allium sativum (garlic) which belongs, to the Liliaceae family was used at concentration 0, 1%, 5% and 10% to know their effect on the growth of fungi poisonous and polluted for waters Aspergillus flavus and Rhizopus oligosporium that isolated from Tigris river 2010. These species were purified and culturing in the laboratory for using in this study. Essential oil of garlic exhibited highly efficiency percent in the inhibition growth of the A. flavus at 100% in the concentration of 10% and growth of the same species at the same concentration was delayed to the fourth day from treatment. However, the growth of R. oligosporium was decrease by using these three concentrations of essential oil. These results were agreed with the results dry weight of fungi that measured decreasing in it with the increasing of concentration of oil at significant difference. To test the effect concentrations of oil on the growth of tested fungi by reading of optical density (OD) which showed that all the concentration caused significant difference (P<0.05( for two species fungi as compared with the control. Reading of OD was depending on the spores concentration in the broth culture, 5%, 10% of oil concentration were caused highly significant differences in the spores concentration as compared with the control. The results pointed on the complete inhibition of fungal growth by garlic in the sample of wastewater inoculated by tested essential oil as compared with the control group (57.33± 2.08) colony/ml.
