Journal of University of Anbar for Pure Science

ISOLATION OF IDENTIFICATION OF KLIBSILLA OXYTOCA FROM RAMADI SOIL AND DETERMINE ITS ABILITY TO NITROGEN FERTILIZER RECOMMENDED FOR COWPEA PLANT

AHMED M.TURKY

Volume 4, Issue 2 , August 2010, , Page 1-12

https://doi.org/10.37652/juaps.2010.15275

Abstract

:The present study sheds light on knowing the spread of klibsilla oxytoca Bacteria in (100) soil sample taken from the regions of AL-Jazeera ,AL-Taameem, AL-Khaldiya , AL-Sufiya and ... Read More ... :The present study sheds light on knowing the spread of klibsilla oxytoca Bacteria in (100) soil sample taken from the regions of AL-Jazeera ,AL-Taameem, AL-Khaldiya , AL-Sufiya and AL-Habbaniya .The ratio of the existence of these Bacteria ranges between 12-88% . The results indicate that all samples contain klibsilla oxytoca Bacteria ;and (36) isolate has been obtained form these Bacteria . The study also includes the evaluation of the effeiency of isolates in foxing Nitrogen .It is appeared that the highest quantity of foxing Nitrogen reaches at (1.3) mlg Nitrogen / 100 ml . by local isolation klibsilla oxytoca J3 .When studying the effect of different concentrations of NP and Urea fertilizers on the growth of isolate in the solid and liquid cultures from the vrem point of colonies diameters and numerical density, the result show the ability of this isolate on the growth at the addition of a quarterly concentration (83 and3.2) per million to NP fertilizer and Urea fertilizer respectively . the diameter .Colonies that grow in the solid culture is ( 2,2,5,3,2,3,4,4,and 3) ml ,respect rely ,during the periods g incubation (144,120,96,72,48,and 24)hour . While the nureoced density reaches at (9.21,9.23,8.98,8.74,7.93,6.98)Log/ml .The results eg .Biology experiment proved the efficiency of this isolate in foxing Nitrogen . Appeared in all the features g plant , (plant weight ,plant length , plant content of nitrogen and chlorophyll ) ,that are studded in the treatment in which isolate mixture is used with quarter of concentration (3.2, 83) per million to NP fertilizer and Urea fertilizer respectively . The results state that the mixture of isolate with a quarter of recommendation is able to foxing Nitrogen at a rate of 4.43 mlg Nitrogen / plant . while the soil content of total Nitrogen is 120.63 mlg/kg and the 8.09 microbial density Log/g soil . It is found that the best rote to these plant ,in this treatment reaches (20 , 74 )in the plant bight and rote con respectively and the dry weight reaches at ( 178 ,16.2 ) mg /plant

BACTERIOCIN ISOLATION FROM SELECTED BACTERIAL ISOLATES AND STUDY 4 ITS INHIBITION ZONE AGAINST SOME PATHOGENIC BACTERIA SPECIES.

RAFAT H. AL-HADITHI; AHMED M.TURKY; LAITH M. NAJEEB

Volume 1, Issue 2 , August 2007, , Page 14-22

https://doi.org/10.37652/juaps.2007.15301

Abstract

استهدفت هذه الدراسة عزل و تشخیص بکتیریا حامض اللاکتیک من 30 عینة اشتملت على اللبن و المخللات و اختبرت ... Read More ... استهدفت هذه الدراسة عزل و تشخیص بکتیریا حامض اللاکتیک من 30 عینة اشتملت على اللبن و المخللات و اختبرت هذه العزلات فی قدرتها على إنتاج البکتریوسین و فعالیته فی تثبیط نمو بعض أنواع البکتیریا المرضیة . کما اشتملت الدراسة اختبار مدى ثباتیة البکتریوسین تجاه المعاملات الحراریة . أظهرت نتائج دراستنا الحالیة قدرة ثلاث عزلات من البکتیریا المستخدمة فی الدراسة على إنتاج البکتریوسین . و أثبتت الاختبارات التشخیصیة المزرعیة و المجهریة و الکیموحیویة التی أخضعت لها هذه العزلات أن عزلتین منها تعود إلى بکتیریا حامض اللاکتیک تحمل الارقام 2 , 14 . أظهرت نتائج الاختبار وجود تباین واضح فی قدرة راشح هذه البکتیریا على تثبیط نمو عزلات البکتیریا المرضیة . أظهرت العزلات الثلاثة فعالیة واضحة فی تثبیط نمو بکتیریا Staphylococcus بینما أظهرت العزلتین رقم 11 و 14 فعالیة تثبیطیة تجاه بکتیریا Streptococcus تتراوح أقطار التثبیط بفعل البکتریوسین لراشح العزلات الثلاثة من بکتیریا الاختبار (بعد استبعاد المواد المثبطة الأخرى بطریقة التسخین) على بکتیریا Staphylococcus قید الدراسة من تثبیط بشکل محدود (العزلتان 3 و 14) إلى تثبیط بقطر 12 ملم (العزلة 5) . یتمیز البکتریوسین المنتج بهذه الدراسة بثباته للفعالیة التثبیطیة عند معاملته بـ 60 °م و لمدة نصف ساعة. عند زیادة فترة التحضین للمزرعة السائلة للبکتیریا نلاحظ زیادة الفعالیة التثبیطیة للمواد المثبطة للنمو بشکل عام.

Journal of University of Anbar for Pure Science

Articles in Press

Current Issue

Volume 17 (2023)

Volume 16 (2022)

Volume 15 (2021)

Volume 14 (2020)

Volume 13 (2019)

Volume 12 (2018)

Volume 11 (2017)

Volume 10 (2016)

Volume 9 (2015)

Volume 8 (2014)

Volume 7 (2013)

Volume 6 (2012)

Volume 5 (2011)

Volume 4 (2010)

Volume 3 (2009)

Volume 2 (2008)

Volume 1 (2007)

Author = M.TURKY, AHMED

ISOLATION OF IDENTIFICATION OF KLIBSILLA OXYTOCA FROM RAMADI SOIL AND DETERMINE ITS ABILITY TO NITROGEN FERTILIZER RECOMMENDED FOR COWPEA PLANT

Abstract

BACTERIOCIN ISOLATION FROM SELECTED BACTERIAL ISOLATES AND STUDY 4 ITS INHIBITION ZONE AGAINST SOME PATHOGENIC BACTERIA SPECIES.

Abstract