Journal of University of Anbar for Pure Science

Detection of ( LasI ) Gene in Pseudomonas aeruginosa and its Role in Biofilm Formation.

Muthanna H. Hassan; Hassan F. Lahig; Luma A. Yassir

Volume 11, Issue 3 , December 2017, , Page 34-39

https://doi.org/10.37652/juaps.2017.145534

Abstract

The present study included the collection of 120 samples from various clinical sources. The samples collected from some hospitals in Baghdad city which included burns ,wound , cystic ... Read More ...

Molecular Detection of Pseudomonas aeruginosa and Study the Effect of Fresh Garlic juice on Some Virulence Factors of this Bacteria.

Hassan F. Lahig; Muthanna H. Hassan; Luma A. Yassir

Volume 11, Issue 2 , August 2017, , Page 9-17

https://doi.org/10.37652/juaps.2017.141584

Abstract

The present study included the collection of 169 samples from various clinical and environmental sources for investigation of existence of Pseudomonas aeruginosa in those sources, the ... Read More ... The present study included the collection of 169 samples from various clinical and environmental sources for investigation of existence of Pseudomonas aeruginosa in those sources, the clinical samples collected from some hospitals in Baghdad city and included 126 samples of wound, burns, cystic fibrosis, ear infections and urinary tract infections (35, 52,4, 22 and 13) samples respectively, while environmental samples were distributed by 25 samples from soil, and 18 samples from wastewater and some swamps. Samples were identified based on the morphological and microscopical characteristics of the colonies when they were culturing in a number of culture media as well as biochemical tests. The molecular detection was used to confirm diagnostic test for the isolates based on the 16SrRNA gene, as a detection gene, which has specific sequences for DNA of P.aeruginosa bacteria.The results of molecular detection showed that 51 isolates belong to target bacteria were distributed as 20 of burns, 9 isolates of wounds, 7 isolates of ear infection, 4 isolates of cystic fibrosis, 2 isolates of urinary tract infection, 5 isolates of soil and 4 isolates of water. The results of phenotypic detection of some virulence factors showed that 49 isolates with percent (95%) produced beta-hemolysis, while 46 isolates (90%) showed variance in the strength of pycocyanin production with a absorbance ranging from (0 to 0.52) at 690 nm, and 47 (92%) of isolates were producing protease enzyme, 44 (86%) of the isolates showed a differences in their ability to biofilm formation. As a result of the increased resistance of P.aeruginosa bacteria towards antibiotics, it is became a problem in the treatment of the infectious diseases caused by this bacteria therefore, the current study focused on the use of fresh garlic extract towards of some virulence factors because it being easy and commonly used. The results of the statistical analysis showed a significant difference when P-value (<0.05) in the production of some virulence factors, the isolates lost their ability to form biofilm, and decreased their ability to produce protease enzyme, and pyocyanin pigment, while did not affect the production of hemolycin.

اختبار کفاءة تحلل الهیدروکربونات بواسطة بکتریا معزولة من ترب ملوثة بالمشتقات النفطیة

Volume 9, Issue 3 , December 2015, , Page 121-129

https://doi.org/10.37652/juaps.2015.127621

Abstract

Molecular genetic study of Pseudomonas aeruginosa DNA repair system

Waleed Khalid Mohammed

Volume 7, Issue 1 , April 2013, , Page 49-56

https://doi.org/10.37652/juaps.2013.83134

Abstract

Bacteria Pseudomonas aurginosa, E coli and Stapthylococcus aureus were exposure to different doses of ultraviolet radiation and survival curves drawn for each type, the results show ... Read More ... Bacteria Pseudomonas aurginosa, E coli and Stapthylococcus aureus were exposure to different doses of ultraviolet radiation and survival curves drawn for each type, the results show that the bacteria Pseudomonas aurginosa more resistant by UV radiation than Ecoli and Stapthylococcus aureus bacteria. The bacterium Pseudomonas aeruginosa was irradiated with different doses of U.V light via wave length( 254 nm ) for different periods ( 50 , 100 , 150 , and 200 sec ) .It appear that part of irradiated bacterial culture was exposed to sun light and the other part was kept in the dark . The survivors of the cells exposed to the sun light was more than the dark and this ensure possessing the bacterium photoreactivating repair system investigate the excision repair system, the minimal inhibitory concentration ( MIC) of caffeine against bacteria was studied by exposing the bacterium to different concentrations of caffeine (10 , 15 , 20 and 25 mg/ml ) and the MIC was 20 mg/ml , Furthermore the bacterium was exposed to different times of U.V. light in the presence of caffeine and the studying ensure that the survivors of the cells in the medium with caffeine was less than the medium with absence of caffeine and this leads to possess the bacterium excision repair system. To detect the recombination repair system , the bacterium was exposed to the concentrations( 0.1 ,0.2 , 0.3 , 0.4 μg / ml) of acrivlavine and the MIC was 0.3 μg / ml , then the bacterium was exposed to different times of U.V. light in the presence of acrivlavine . The survivors of the cells in the medium with acrivlavine was less compared with the absence of acrivlavine. It would seem that possessing bacterium recombination repair system . sensitivity test of the bacterium against antibiotics was established and the results appeare that it was to the antibiotics Chloramphenicol, Carpencillin, Trimethoprim, Rifampicin The diameters of inhibition were (16,20,17,18) mm respectively and resistant to the antibiotics Amoxicillin, Ampicillin, Clindomycin, Cloxacillin , Nalidixic acid , Cephaloxin , Tetracyclin and Tobromycin. To study SOS repair system the bacterium was mutated with direct mutagens represented with nitrous acid and indirect mutagens represented with U.V. light to isolate Rifampicin and Chloramphenicol mutants. It is quite likely that the sensitivity of bacterium for mutagenesis then possessing SOS repair system .

Study the effect of Antibiotics on pyocyanin production from Pseudomonas aeruginosa and pyocyanin as Antibiotic against different pathogenic bacteria

Enass G. Sweedan

Volume 4, Issue 1 , April 2010, , Page 15-18

https://doi.org/10.37652/juaps.2010.43881

Abstract

aeruginosa was cultured on nutrient agar containing different antibiotics,the growth and pigment of bacterial colonies were treated. Azithromycin killed the bacteria on low concentration,colonies ... Read More ...

PURIFICATION AND CHARACTERIZATION OF PROTEASE FROM Pseudomonas aeruginosa ISOLATED FROM SOME WOUND AND BURN INFECTION.

SAMEER F. SAMAAN; SOUOD R. ALANI; RANA M. AL-SHWAIKH

Volume 3, Issue 3 , December 2009, , Page 37-44

https://doi.org/10.37652/juaps.2009.15593

Abstract

Twenty Four isolates of Pseudomonas aeruginosa were identified. The isolates were 8(33%) from wound infections, 16(66%) isolates from burn infections. The sensitivity of Pseudomonas ... Read More ...

Isolation of Ps. aeruginosa and study of resistance to some antibiotics and ability to pyocins production.

Salah S. Zain-Al-Abeddin; Daffer F. Al-Rawi; Sohaib S. Qaseem

Volume 3, Issue 1 , April 2009, , Page 1-9

https://doi.org/10.37652/juaps.2009.15576

Abstract

The study involved the isolation and identification of Pseudomonas aeruginosa , thirty four (34)isolates obtained from (77)samples that were collected from patients attending Kirkuk ... Read More ...

QUANTITATIVE AND QUALITATIVE ASSAYS OF BACTERIAL BIOFILM PRODUCED BY Pseudomonas aeruginosa AND Klebsiella spp.

Shaymaa H. Moteeb

Volume 2, Issue 3 , December 2008, , Page 6-13

https://doi.org/10.37652/juaps.2008.15572

Abstract

The aim of this study was to detect biofilm formation by study isolates of Pseudomonas aeruginosa and Klebsiella spp. qualitatively and quantitatively. Twenty-five isolates were taken ... Read More ...

MUTAGENESIS OF A Pseudomonos aeruginosa By USING NITROUS ACID

Khalaf Jasim Mohammed Al-Dolaimi

Volume 1, Issue 3 , December 2007, , Page 45-49

https://doi.org/10.37652/juaps.2007.15654

Abstract

The bacterium Pseudomonas aeruginosa was isolated from patients of burns in Al-Ramadi hospital and diagnosed according to its cultural , microscopic and biochemical properties. sensitivity ... Read More ...

MULTIPLE ANTIBIOTIC RESISTANCE OF PSEUDOMONAS AERUGINOSA ISOLATED FROMAL-RAMADI CITY.

Salah S.Z Al-Jarrah

Volume 1, Issue 1 , April 2007, , Page 9-14

https://doi.org/10.37652/juaps.2007.15404

Abstract

One hundred clinical isolates of Pseudomonas aeruginosa were isolated from 230 clinical samples taken from inpatients and out patient in general hospital in Al-Ramadi City during 2001 ... Read More ...

Volume 17 (2023)

Volume 16 (2022)

Volume 15 (2021)

Volume 14 (2020)

Volume 13 (2019)

Volume 12 (2018)

Volume 11 (2017)

Volume 10 (2016)

Volume 9 (2015)

Volume 8 (2014)

Volume 7 (2013)

Volume 6 (2012)

Volume 5 (2011)

Volume 4 (2010)

Volume 3 (2009)

Volume 2 (2008)

Volume 1 (2007)

Keywords = Pseudomonas aeruginosa

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