Journal of University of Anbar for Pure Science

Cultivation and Detection of Unculturable Fungi in Soil Using Soil Infusion Agar(SIA)

Wijdan Ahmed Ali; Bahrouz M.A.AL-Jaff; Sajid S.Saleem AL-saeedi

Volume 12, Issue 1 , April 2018, , Page 9-18

https://doi.org/10.37652/juaps.2022.171439

Abstract

Unculturable and non sporulating fungi represent a great challenge in studying biotrophic ,endotrophic and mycorrhizal fungal groups. In this study a novel culture dependent technique ... Read More ...

Detection of acquired resistance genes for vancomycin in local isolates Escherichia coli and its relationship with other resistance models

Shahad Hisham Mahmood; Ahmed Mohamed Turkey; Ilham Abdulhadi Khalaf

Volume 11, Issue 3 , December 2017, , Page 1-14

https://doi.org/10.37652/juaps.2017.145219

Abstract

This study included the collection of 374 samples (distributed according to the source of collection ) as clinical samples (urine, wounds, burns, feces) from Ibn – Al – ... Read More ...

Molecular identification of some virulence related genes for E. coli O157:H7 isolated from bloody diarrhea and UTI in Baghdad city.

Mohamed B. Al-Jobory; Amina N. Al-Thwaini; Laith M. Najeeb

Volume 11, Issue 3 , December 2017, , Page 17-25

https://doi.org/10.37652/juaps.2017.145253

Abstract

This study was carried out for detection of some virulence factors for E coli O157:H7 isolated from patients with bloody hemorrhagic diarrhea or urinary tract infection (UTI). A total ... Read More ...

Detection of genotypes for Giardia lamblia in Iraqi patients feces by using PCR-RFLP techniques based on GDH gene characterization

Sabaa M. Al-Fahadawi; Sarab F. Al-Ani; Yusra G. Yaseen

Volume 11, Issue 2 , August 2017, , Page 18-24

https://doi.org/10.37652/juaps.2017.141585

Abstract

This study was carried out during the period from March 2016 to January 2017 to detect of genotypes and subgenotypes of Giardia lamblia in human feces by PCR-Restriction fragment length ... Read More ...

Genotyping Of ABO gene In Iraqi Population Sample By Using PCR-RFLP Technique

Qabas H. Alawee; Louay M. Abdou; Saad M. AL-Nida

Volume 10, Issue 3 , December 2016, , Page 20-29

https://doi.org/10.37652/juaps.2016.134879

Abstract

The aim of this study was the molecular detection of ABO gene and identify the patterns of alleles in Iraqi population sample. The study consisted of 100 unrelated individuals who appear ... Read More ...

دراسة التباین الجزیئی للعزلات المرضیة لبکتریا الزوائف الزنجاریة لجینات ,phzM , toxA ,pvdE 16srRNA الخاصة بالضراوة والصفات التشخیصیة

Ahmed M. Yuosif; Ahmed M. Turkey; Ahmed A. Suliaman

Volume 9, Issue 3 , December 2015, , Page 24-35

https://doi.org/10.37652/juaps.2015.127584

Abstract

This study includes collection of 438 clinical samples from Ramadi Educational Hospital and 50 of soil samples in period from Nov. 2014 to Feb. 2015, to isolate Pseudomonas aeruginosa ... Read More ...

The relationship between genotype using BOX-PCR technique and lead bioremoval by Klebsiella pneumoniae

Nuhad M. Hamed; Ahmed M. Turkey; Harith J. Fahad; Mahmood M. Al-Mahdawi

Volume 9, Issue 3 , December 2015, , Page 80-89

https://doi.org/10.37652/juaps.2015.127608

Abstract

The effect of metabolic status on lead bioremoval byKlebsiella pneumoniae were tested. The results revealed a significant differences (P<0.05) between live and dead (killed by heat) ... Read More ...

Molecular Variation Study of Clinical Isolates of Pseudomonas aeruginosa for 16SrRNA,pvdE, toxA, and phzM Genes Related with Virulence and Characterization Features.

Ahmed M. Yuosif; Ahmed M. Turky; Ahmed A. Sulaiman

Volume 9, Issue 3 , December 2015, , Page 144-155

https://doi.org/10.37652/juaps.2015.127638

Abstract

This study includes collection of 438 clinical samples from Ramadi Educational Hospital and 50 of soil samples in period from Nov. 2014 to Feb. 2015, to isolate Pseudomonas aeruginosa ... Read More ...

The detection on the infection with the H.pylori by using molecular test (PCR ) by using the different genes and different sampling in the Al-Ramadi city

Dhuha abd Al-salam Obeed; Mohammed Qais Abd; Essam Mohamed Abdullah

Volume 9, Issue 3 , December 2015, , Page 177-184

https://doi.org/10.37652/juaps.2015.127642

Abstract

The study aims at finding the infection of Helicobacter pylori by using the molecular test (polymerase chain reaction ) to finding the genes that most efficient in the diagnosis and ... Read More ...

Physiological and Molecular Study for Uromodulin Gene Effect and its Relation with Recurrent UTI in Sample of Anbar Province Females

Hanan H. saleh; Samir M. Khalif; Ahmed A. Suleiman

Volume 9, Issue 2 , August 2015, , Page 56-62

https://doi.org/10.37652/juaps.2015.124510

Abstract

The current study Included (100) women suffering from recurrent infection of the urinary tract revisions for hospitals and civil laboratories in Anbar province, the category of age ... Read More ...

Cystic and Alveolar Echinococcosis: Molecular survey in Al-Anbar Province

Sarab F. Al-Ani; Mohamed T. Dagash; Mahmood Jasem Al-Shabani

Volume 8, Issue 2 , August 2014, , Page 9-13

https://doi.org/10.37652/juaps.2014.123910

Abstract

Forty specimens of surgically removed hydatid cyst were obtained from patients in different hospitals in Al-Anbar province. The aim of study was designed to determine the type of common ... Read More ... Forty specimens of surgically removed hydatid cyst were obtained from patients in different hospitals in Al-Anbar province. The aim of study was designed to determine the type of common larval stage (hydatid cyst) of echinococosis in Al-Anbar province according to PCR molecular differentiation.The study was carried out during the period from first day of January to the last day of December 2013.The age of patient ranged from (10 – ≥ 65 ) years old, (19 males and 21 females). A questionnaire paper was performed to each patient which includes information about age, sex,residence,occupation,exposure variable, water source, site of hydatid cyst and case history.From the obtained results, it's revealed that the age group (21 – 30 years) presented high hydatidosis parasitic infestation which was 35% , whereas the age group more than50 years presented low parasitic infection which was 2.5% . 47.5% of patients were males and 52.5% were females.Twenty eight(70%) patients live in rural areas. Also, twenty patients (50%) were exposured to livestock animals, (40%) of patients were farmers, twenty seven patients(68%) were consumed river water, The most common localization was the liver (65%). Purification of DNA was done by QIAamp DNA Mini kit from QIAGEN for cyst. Specific primers were designed for the diagnostic PCR. It amplifies a 133-basepair segment within the newly described repeated unit that amplified a target repeated sequence (EgG1 Hae III) newly identified in the genome of the common sheep strain of E. granulosus. Primer based on the flanking sequences allowed the PCR amplification of E. multilocularis, a fragment of mitochondrial 12S rDNA was amplified by PCR from human genomic DNA by using the cestode-specific primers 60 were used as targets for (PCR) amplification.The total number of the cysts specimens that gave positive results of E.granulosus by PCR was 37 (92.5%) patients and the number of specimens that gave negative results was 3(7.5%),while all specimens gave negative result of E. multilocularis by using PCR analysis.The present study showed that E. granulosus is a major causative of hydatidosis in Al-Anbar Province while E. multilocularis is not detected by using PCR approach.

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GENETIC DIVERSITY ANALYSIS FOR NUMBER OF DATE PALM VARIETIES IN AL-ANBAR USING THE RAPD-PCR TECHNIQUE

KHALID JAMAL HASHIM; ABDUL MAJEED A. HUMADI

Volume 8, Issue 2 , August 2014, , Page 31-39

https://doi.org/10.37652/juaps.2014.123975

Abstract

The present study deals with the application of RAPD markers to study ten varieties of date palm in order to determine the genetic relationship and genetic distance among them. also ... Read More ...

DETERMINING THE GENETIC FINGERPRINT FOR NUMBER OF (Triticum aestivum ) AGRICULTURAL SPECIES DISTRIBUTE IN AL-ANBAR PROVINCE USING THE ISSR TECHNIQUES MARKER

ABDUL-MAJEED A. HUMMADI; ODAY H. NOORI

Volume 8, Issue 1 , April 2014, , Page 48-58

https://doi.org/10.37652/juaps.2014.122638

Abstract

In this study, We used the ISSR Technique, Its one of the PCR Technique, On the nine local type of Triticum aestivum in AL-anbar Province, to determination of genetic variation relationship ... Read More ...

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Molecular characterization of Pseudomonas aeruginosa Isolates Isolated from Clinical Patients by Using RAPD-PCR Technique.

Akeel H. AL-Assie; Adnan F. Al-azawy; Wakas S. Al-Dori

Volume 6, Issue 3 , December 2012, , Page 27-35

https://doi.org/10.37652/juaps.2012.78439

Abstract

The aim of the present study was the molecular characterization and the evaluation of variability and genetic relationship of six Pseudomonas aeruginosa isolates using PCR-based Randomly ... Read More ... The aim of the present study was the molecular characterization and the evaluation of variability and genetic relationship of six Pseudomonas aeruginosa isolates using PCR-based Randomly Amplified Polymorphic DNA (RAPD) technique. A total number of 86 samples were collected from patients that hospitalized in Tikrit Teaching Hospital in Tikrit city. These samples were taken from patients basing on the sources of infections, the isolates were taken from: wounds, ear, burns, urine, sputum, and eyes infections. Using enrichment, selective media, and biochemical tests, that characterized and identified as P. aeruginosa. Genomic DNA was extracted from six P. aeruginosa isolates isolated from these different sources. These genomic DNA samples were found to have a suitable concentration and purity for RAPD-PCR analysis. RAPD-PCR technique was performed using 15 different Operon random primers. Eleven primers gave successful amplification results in repeated experiments. As a result, the total number of amplified bands was 270 and the total number of polymorphic bands was 234. The highest number of polymorphic bands (39 bands) was produced by primer OPX-01. The primer efficiency ranged from 3.70 (primer OPA-11) to 14.44 (primer OPX-01) and the discriminatory value ranged from 1.70% (primer OPA-11) to 16.66% (primer OPX-01). In addition, genetic distance and cluster analysis among different P. aeruginosa isolates were estimated by using UPGMA computer program basing on RAPD-PCR banding patterns that obtained in this study. These results suggesting that possible and frequent occurrence of mutations in DNA sequencing P. aeruginosa bacteria from different sources and locations. This study has proved existence genetic differences (DNA polymorphism) among the six P. aeruginosa isolates isolated from different sources. Therefore, we can say that RAPD technique could be an efficient technique for studying the molecular characterization and the epidemiology of P. aeruginosa bacteria.

Volume 17 (2023)

Volume 16 (2022)

Volume 15 (2021)

Volume 14 (2020)

Volume 13 (2019)

Volume 12 (2018)

Volume 11 (2017)

Volume 10 (2016)

Volume 9 (2015)

Volume 8 (2014)

Volume 7 (2013)

Volume 6 (2012)

Volume 5 (2011)

Volume 4 (2010)

Volume 3 (2009)

Volume 2 (2008)

Volume 1 (2007)

Keywords = PCR

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