DETERMINING THE GENETIC FINGERPRINT FOR NUMBER OF (Triticum aestivum ) AGRICULTURAL SPECIES DISTRIBUTE IN AL-ANBAR PROVINCE USING THE ISSR TECHNIQUES MARKER
AbstractIn this study, We used the ISSR Technique, Its one of the PCR Technique, On the nine local type of Triticum aestivum in AL-anbar Province, to determination of genetic variation relationship between them and Find the Fingerprinting for some types. The DNA Extraction Results From young leaves by using CTAB method showed that purity of DNA was 1.5 – 1.82 and the concentration was 55 – 142.5 ngµl. The ISSR Results revealed 8 of the 10 primers using this study were binding. The 8 primers recorded 404 binding sites ( 126 General bands and 278 various bands ), The highest number for binding was in L1 primer ( 78 band ) while B1 primer was the lowest number ( 30 band ), The high molecular Wight was 2000 bp for H14 primer, On the other band the lowest molecular Wight was 500 bp in E3 primer. The type Latifya ( 1 ) has highest number for binding site with 55 bands 13.613% the type Iraq Shown lowest number of binding site with 39 bands 9.563 % For the unique and absent band , the type ( Tertikely ( 2 ) and Abo Graib ( 4 ) ) and ( latifya ( 1 ) and Hashimya ( 3 ) ) Recording highest and lowest number of band ( 5 and 3 ) respectively, Whereas the type Tamooz 3 ( 7 ) and Ibaa 95 ( 8 ) had no unique and absent bands. For the genetic variation for ISSR, we found the highest value between Tertikely ( 2 ) and Tamooz 2 ( 6 ) was ( 0.4753 ) and the lowest value was ( 0.1707 ) between latifya ( 1 ) and Hashimya ( 3 ) depending for our Results we divided. The Studying to 3 groups, and the Tertikely ( 2 ) type presents the main group between among other type.
- PCR ، ISSR
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