Document Type : Research Paper
Authors
College of Science, University of Anbar
10.37652/juaps.2014.122635
Abstract
Isolated and well identified K. pneumoniae, was included in this study the sensitivity to some antibiotics and the impact of certain natural products (honey and garlic) against these bacteria and Impact Statement synergistic action of these products with antibiotics, as the results showed that interference honey and garlic juice with antibiotics significant effect in increasing the sensitivity of the K. pneumoniae to antibiotics, and were grown K. pneumoniae in the nutrient broth containing different concentrations of honey and garlic juice, and tested the susceptibility for the same antibiotics mentioned. The isolation of K. pneumoniae is used in this study of humoral immune indicators. As a method is used Single Radial Immunodiffusion Test Kits to measure the levels of antibodies and complement proteins, the results showed a significant increase in the level antibodies types IgA, IgM, IgG and complement proteins C3, C4 for immunization groups compared with positive and negative control groups.
Main Subjects
1- Hansen, D. S. ; Aucken, H. M. ; Abiola, T. and Podschun, R.(2004). Recommanded Test Panel For Differentiation Of Klebsiella Species on The Basis Of Trilateral Interlaboratory Evaluation Of 18 Biochemical Tests . J . Clin . Microbiol . 42(8):3665-3669 .
2- Umebobiamiwa, L.B. and Berkwitz, M.D.(2009).Klebsiella infection Klebsiella spp. as nosocomial pathogens : epidemiology , taxonomy , typing methods, and pathogenicity factors. Clin.Microbial.Rev, 11(4):589-603.
3- Baron, S. (2004). Medical Microbiology. 4th Ed. (Cited In 2004, Julay / 24). Available From : NCBI .Galveston .USA.
4- Graham, Jay P.; Lance B.; Price, Sean L. Evans,; Thaddeus K.; Graczyk,. and Silbergeld Ellen K.(2009). Antibiotic Resistant Enterococci and Staphylococci Isolated From Flies Collected Near Confined Poultry Feeding Operations. STOTEN-11054; No Of Pages 10.
5- Kwakman P. H. S.; De Boer, L. ; Ruyter-Spira, C. P. ;Creemers-Molenaar ,T. ; Helsper, J. P. F. G. ; Vandenbroucke-Grauls , C. M. J. E. ; Zaat, S. A. J. ; and Te Velde A. A. (2011). Medical-Grade Honey Enriched with Antimicrobial Peptides has enhanced activity against antibiotic-resistant Pathogens. Eur J Clin Microbiol Infect Dis., 30(2): 251–257.
6- Frey, FM., and Ryan, M.(2010). Antibacterial Activity Of Traditional Medicinal Plants Used by Haudenosaunee Peoples Of New York State. BMC Complem Altern Med;10:64.
7- Obi, C.L.; Ugoji, E.O.; Edun, S.A.; Lawal, Anyiwo, S.F. and Anyiwo, C.E. (2000). The Antibacterial Effect of Honey on Diarrhea. Causing Bacterial Agent Isolated In Lagos, Nigeria, African J. of Med Sci. 23:257-260.
8- Amagase H, Petesch BL, Matsuura .(2001) Intake of Garlic and Its Bioactive Components.J Nutr.;131(3s):955S-62S.
9- Miron,T.; Rabinkov,A.; Mirelman,D.; Wilchek,M. and Weiner, L. (2000). The mode of action of allicin: its ready permeability through phospholipid membranes may contribute to its biological activity. Biochim Biophys Acta. ;1463(1):20-30.
10- Powrie, F. and Coffman, R.L. (1993). Cytokine regulation of T-cell function: potential for the therapeutic intervention. Immunol. 14: 270-274.
11- Macfaddin, J.F.(2000). Biochemical Tests For Identification of Medical Bacteria . 3 rd ed. Lippincott , Williams And Wilkins . Philadelphia . London .
12- Collin, C.H. ,Grange, J.M. and Lyne, P.M.(1989). Microbiological Methods .6thed.Butter Worth's and Co. Publication .U.K.
13- Vandepitte J; Engback K; Piote P; and Heuk, C. (1991). Basic Laboratory Procedures In Clinical Bacteriology. World Health Organization, Geneva
14- Bauer, A. W.; Kirby, W. M.; Sherries, J. C. and Turck, M. (1966). Antibiotic Susceptibility Tests by A Standarized Single Disk Method. Am. J. Clin. Path. 45:493-496.
15- Reuter HD, Koch HP, and Lawson LD. (1996). Therapeutic Effects and Applications of Garlic and Its Preparations. In: Garlic. The Science and Therapeutic Application Of Allium sativum L and Related Species (Koch HP, Lawson LD, Eds.), Williams and Wilkins, Baltimore, Pp. 135-512.
16- Jarosz , J. and Lipa , J.J. (1990). Fungistatic an Fungicidial activity of Albarep and garlic juice against phytopathologenic fungi . Rocz . Nauk Rol . Ser . E .20:53-59.
17- Hames, B. and Rickwood, D.(1984). Gel Electrophoresis Of Proteins, Apractical Approach . (3rd Ed.) . IRL Press Limited . England .
18- Mancini, G.; Carbonara, A.O. and Hermans, J.F. (1965). Immunochemical Quantization of Antigen by Single Radial Immunodiffusion. Immu .Che., 2:235-254.
19- Jenkins R., Cooper R.(2012) Improving Antibiotic activity against wound Pathogens with Manuka Honey In Vitro. Plos one 7(9): E45600.
20- Collee, G. J.; Faser, G. A.; Marmion, B. and Simmons, A. (1996). Mackie and Mccartney. Practical Medical Microbiology.14thed. Churchill Livingstone, New York.
21- Ivana, Ć., Jovalekic, M. and Jegorovic, B.(2012). In Vitro Antibacterial Activity of Garlic and Synergism between Garlic and Antibacterial Drugs. Arch. Biol. Sci., Belgrade, 64 (4), 1369-1375 .
22- Palaksha, M.N., Mansoor Ahmed, and Sanjoy Das.(2010). Antibacterial activity of garlic extract on streptomycin-resistant Staphylococcus aureus and Escherichia coli solely and in synergism with streptomycin. Vol : 1 Issue : 1 Page : 12-15
23- Mirzoeva, O. K., Grishanin, R. N., and P. C. Calder (1997). Antimicrobial Action Of Propolis and Some of Its Components: The Effects On Growth, Membrane Potential and Motility Of Bacteria. Microbiol. Res. 152, 239–246.
24- Betoni JE, Mantovani RP, Barbosa LN, Di Stasi LC, and Fernandes Junior A.(2006). Synergism Between Plant Extract and Antimicrobial Drugs Used On Staphylococcus aureus Diseases. Mem. Inst. Oswaldo. Cruz.101: 387-390.
25- Fry FH, Okarter N, Baynton-Smith C, and Kershaw MJ, .(2005). Use Of A Substrate/Alliinase Combination To Generate Antifungal Activity In Situ. J Agric Food Chem. 9; 53: 574-580.
26- Kathleen, A. Carrado, Argonne National Labs.(2004): Honey, It's Chermistry. The Food Network Alton Brown's Good Eats Episode On Honey.
27- Gilmour, R.; Messner, P. Guffanti, A.A.; Kent, R.;Scheberl, A.; Kendrick, N. and Krulwich, T.A.(2000): Two-Dimensional Gel Electrophoresis Analyses Of Ph-Dependent Protein Expression In Facultatively Alkaliphlic Bacillus Pseudofirmus OF4 Lead To Characterization Of An S-Layer Protein With A Role In Alkaliphily. J. Bacteriol..P. 5969-5981.
28- Hassanain A.T., Alyaa A.K., and Karim A.J.(2010). Antimicrobial Effect of Malaysian Honey on Some Human Pathogens: An In Vitro Study. Int Med J Malaysia; 9(2): 15-18.
29- Al-Ani, Najeeb, Hussain, Fayyadh, Abdul-Razik, and Ali.(2011). Study Of Some Immunological Changes Resulted From Vaccination Of Local Rabbits With Attenuated Klebsiella .Al-Anbar J. Vet. Sci.,Vol;4 No2.
30- Mc Sorly ;S.J.S. and Castalonga M. (2002). Tracking Salmonella specific CD4T cells in vivo reveals a local Mucisal response to a disseminated infection immunity., 16:328.
31- Alaa , J. Hassan, Shakir, H. Al-alwany and Hassan F. Naji. (2007). Humoral and Cellular Immune Response against Escherichia coli In Vivo. Med Jo.of Babylon. Vol. 4 No.3 and 4.
32- Hussain, A. B.; Fiadh, H. M.; Almaadhidy, A. H.; Najeb, L. M. and Shaker, E. A. (2010). Study of some immunization effect against attenuated Pseudomonas aerugenosa In Local Rabbits. Al-Anbar J.Vet.Sci., 3(1): 83- 90.
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