Document Type : Research Paper

Authors

College of Science / University of Anbar

10.37652/juaps.2015.127586

Abstract

85 samples were collected to investigate Pseudomonas sp. bacteria and study their resistance to antibiotics. The samples were included (18 specimen of wounds, 21 of burns, 23 specimen otitis media, 6 of urine tract infact, 3 of sputum) and soil samples . These cases were diagnosed using cultural and biochemical tests, the diagnosis was confirmed using the API 20E system. The results showed high isolation from burns and otitis media (23%) . The species was obtained Pseudomonas aeruginosa 49 (94.2%) followed by the bacteria Pseudomonas fluorescens 2 (3.8%), Pseudomonas oryzihabitans 1 (1.9%). Resistance of Pseudomonas sp. isolates to antibiotics were examined to 10 antibiotics, and isolates showed highest sensitivity to antibiotics Imipinem, Ciprofloxacin, Amikacin 98.1% and 92.3% and 90.4%, respectively. Isolates were showed high resistant (88.5%) to Cefotaxime .

Keywords

Main Subjects

  1. Ferguson,D.(2007). A study of clinical strains of Pseudomonas aeruginosa and the investigation of antibiotic resistance mechanisms in the multidrug resistant strain PA13. School of Biotechnology and National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Ireland
  2. Franzetti L and Scarpellini M (2007). Characterisation of Pseudomonas spp. isolated from foods. Annals of Microbiology. 57(1): 39-47.
  3. Moore E. R. B.; Tindall B. J.; Santos V. A. P. M. D; Pieper D.H.; Ramos J-L; Palleroni N.J.(2006). Nonmedical: Pseudomonas. Prokaryotes 6:646–703
  4. Blanc, D. S.; Francioli, P. and Zanetti, G.(2007). Molecular Epidemiology of Pseudomonas aeruginosa in the Intensive Care Units – A Review. The Open Microbiology Journal, 1, 8-11
  5. Gershman M. D., et al. 2008. Multistate outbreak of Pseudomonas fluorescens bloodstream infection after exposure to contaminated heparinized saline flush prepared by a compounding pharmacy. Clin. Infect. Dis.47:1372–1379.
  6. Woo, K-S; Choi, J-L; Kim, B-R; Kim, J-E; Kim, K-H; Kim, J-M; and Han, J-Y. (2014). Outbreak of Pseudomonas Oryzihabitans Pseudobacteremia Related to Contaminated Equipment in an Emergency Room of a Tertiary Hospital in Korea. Infect Chemother;46(1):42-44.
  7. Pagès, Jean-Marie; James, Chloë E; and Winterhalter, Mathias. (2008).The porin and the permeating antibiotic: a selective diffusion barrier in Gram-negative bacteria. naTuRE REvIEws | microbiology, vOl, 6. P: 893-903.
  8. Sugawara, E.; Nagano, K.; and Nikaido, H.(2011). Alternative folding pathways of the major porin OprF of Pseudomonas aeruginosa. FEBS Journal 279, p: 910–918
  9. James, C. E.; Mahendran, K. R.; Molitor, A.; Bolla, J-M.; Bessonov, A. N. et al. (2009). How β-Lactam Antibiotics Enter Bacteria: A Dialogue with the Porins. PLoS ONE 4(5): e5453.
  10. Delcour, A. H.(2009). Outer membrane permeability and antibiotic resistance. Biochim Biophys Acta;1794(5):808-16.
  11. Ferenci, T. and Phan, K.( 2015). How Porin Heterogeneity and Trade-Offs Affect the Antibiotic Susceptibility of Gram-Negative Bacteria. Genes 2015, 6, 1113-1124
  12. Nikaido, H. and Rosenberg, E. Y. (1983) Porin channels in Escherichia coli: studies with liposomes reconstituted from purified proteins. J Bacteriol;153:241–252.
  13. Kobayashi, Y.; Takahashi, I.; and Nakae, T.(1982). Diffusion of beta-lactam antibiotics through liposome membranes containing purified porins. Antimicrob Agents Chemother;22:775–780.
  14. Ochs MM, Bains M, Hancock REW. (2000). Role of putative loops 2 and 3 in imipenem passage through the specific porin OprD of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 44:1983–1985.
  15. Trias, J.; Dufresne, J.; Levesque, R. C. & Nikaido, H. (1989). Decreased outer membrane permeability in imipenem-resistant mutants of Pseudomonas aeruginosa. Antimicrob Agents Chemother 33, 1202–1206.
  16. Lambert, P. A.(2002). Mechanisms of antibiotic resistance in Pseudomonas aeruginosa. Journal of the Royal Society of Medicine 95, Suppl.41, 22–6.
  17. Morita, Y., Tomida, J., and Kawamura, Y. (2012). Primary mechanisms mediating aminoglycoside resistance in the multidrug-resistant Pseudomonas aeruginosa clinical isolate PA7. Microbiology 158, 1071-1083
  18. Masi, M. and Pagès, J-M. (2013). Structure, Function and Regulation of Outer Membrane Proteins Involved in Drug Transport in Enterobactericeae: the OmpF/C – TolC Case. The Open Microbiology Journal , 7, (Suppl 1-M2) 22-33.
  19. Atlas, R.M. (1995). Principles of microbiology. (1st ed.). Mosby Year Book, Inc., Boston, Pp :111-510.
  20. MacFaddin, J. F.(2000). Biochemical Tests for Identification of Medical Bacteria. 3rd ed. Philadelphia:Lippincott Williams and Wilkins; 2000. p. 363-7
  21. Vandpitte, J.; Engback, K.; Piot, P. and Heuck, C.C.(1991). Basic laboratory procedures in clinical bacteriology. WHO., Geneva., PP: 78-110.
  22. Bhatawadekar, S. M.(2013). Community-Acquired urinary tract Jun; J- Glob Infect Dis. pseudomonasApr oryzihabitanS . infection by 5(2): 82–84.
  23. Kodama et al., (1985) “Two New pecies of Pseudomonas: P. oryzihabitans Isolated from Rice Paddy and Clinical Specimens and P. luteola Isolated from Clinical pecimens,” Int J yst acteriol 35: 467-474
  24. Shen, J. L.; Fang, Y. J.(2015). Detection of drug-resistance mechanism of Pseudomonas aeruginosa developing from a sensitive strain to a persister during carbapenem treatment. Genetics and Molecular Research 14 (2): 6723-6732.
  25. Strateva, T and Yordanov, D. (2009). Pseudomonas aeruginosa - a phenomenon of bacterial resistance. J Med Microbiol. 58 (Pt 9):1133–48.
  26. Nestorovich E. M.; Danelon C.; Winterhalter M.; and Bezrukov S. M.(2002). Designed to penetrate: Time-resolved interaction of single antibiotic molecules with bacterial pores. PNAS _ vol. 99 _ no. 15 _ 9789–9794.
  27. Terzi H. A.; Kulah C.; Atasoy A. R.; and Ciftci I. H.(2015). Investigation of OprD Porin Protein Levels in Carbapenem-Resistant Pseudomonas aeruginosa Isolates. Jundishapur J Microbiol. 8(12): e25952.
  28. NCCLS. Performance standards for antimicrobial disc susceptibility tests. NCCLS: Wayne PA;2014. M2-A6.
  29. Japoni A, Alborzi A, Kalani M, Nasiri J, Hayati M, Farshad S. (2006). Susceptibility patterns and cross-resistance of antibiotics against Pseudomonas aeruginosa isolated from burn patients in the South of Iran. Burns;32:343-7.
  30. Tsakris A, Pournaras S, Woodford N, Palepou MF, Babini GS, Douboyas J, Livermore DM.(2000). Outbreak of infections caused by Pseudomonas aeruginosa producing VIM-1 carbapenemase in Greece. J Clin Microbiol, 38:1290-2.
  31. Bertrand X, Thouverej M, Patry C, Balvay P, Talon D. (2001). P.aeruginosa: Antibiotic susceptibility and genotypic characterization of strains isolated in the intensive care unit. Clin Microbiol Infect;7:706-8.
  32. Manikandan, C. and Amsath, A.(2013). Antibiotic susceptibility of bacterial strains isolated from wound infection patients in Pattukkottai, Tamilnadu, India. Int.J.Curr.Microbiol.App.Sci 2(6): 195-203
  33. Karlowsky JA, Draghi DC. Jones ME, Thornsherry C, Friedland IR, Saham DF. Surveillance for antimicrobial susceptibility among clinical isolates of P. aeruginosa and Acinetobacter baumannii from hospitalized patients in the United states, 1998 to 2001. Antimicrob Agents Chemother 2003;47:1681-8.
  34. Rashid, H.; Zeb, M.; Jamal, Q.; Waqar, M ; Farooqi, B. J. and Majid, A.(2014). Frequency and Antimicrobial Susceptibility Pattern of Pseudomonas aeruginosa in Ear Swabs. World Applied Sciences Journal 30 (7): 812-817.
  35. Mansoor, T.; Musani, M. A.; Khalid, G. and Kamal, M. (2009). PSEUDOMONAS AERUGINOSA IN CHRONIC SUPPURATIVE OTITIS MEDIA: SENSITIVITY SPECTRUM AGAINST VARIOUS ANTIBIOTICS IN KARACHI. J Ayub Med Coll Abbottabad;21(2).p. 120-3.
  36. Muluye, D.; Wondimeneh, Y.; Ferede, G.; Moges, F.; and Nega, T.(2013). Bacterial isolates and drug susceptibility patterns of ear discharge from patients with ear infection at Gondar University Hospital, Northwest Ethiopia. BMC Ear, Nose and Throat Disorders 13:10 Page 5.
  37. Ahmad, S.(2013). Antibiotics in chronic suppurative otitis media: A bacteriologic study. Egyptian Journal of Ear, Nose, Throat and Allied Sciences 14, 191–194.
  38. Adel, K.K. and Sabiha, S.S.(2010). Genetic Site Determination of Antibiotic Resistance Genes in Pseudomonas aeruginosa by Genetic Transformation. British Journal of Pharmacology and Toxicology 1(2): 85-89.
  39. Nemat-Gorgani N .(2009). Detecting mutations related to antibiotic resistance in Pseudomonas aeruginosa. Department of Biological Sciences, San Jose State University SJSU ScholarWorks.
  40. Askoura, M.; Mottawea, W.; Abujamel, T. and Taher, I. (2011). Efflux pump inhibitors (EPIs) as new antimicrobial agents against Pseudomonas aeruginosa . Liby. J. Med. 6(6):5870-5878.
  41. Kim, J.Y.; Park, Y.J.; Kwon, H.J.; Han, K.; Kang, M.W. and Woo, G.J. (2008). Occurrence and mechanisms of amikacin resistance and its association with b-lactamases in Pseudomonas aeruginosa: a Korean nationwide study. J Antimicrob Chemother. 62:47983
  42. Kotra LP, Haddad J, Mobashery S (2000) Aminoglycosides: perspectives on mechanisms of action and resistance and strategies to counter resistance. Antimicrob Agents Chemother 44: 3249–3256.
  43. Taneja N, Chatterjee S.S, Singh M, Singh S, Sharma M. (2010) Paediatric urinary tract infections in a tertiary care center from north India. Indian J Med Sci.;131:101-5.