Document Type : Research Paper
Authors
1 Anbar Education Directorate
2 University of Anbar - College of Science
3 University of Baghdad - College of Science
Abstract
The effect of metabolic status on lead bioremoval byKlebsiella pneumoniae were tested. The results revealed a significant differences (P<0.05) between live and dead (killed by heat) cellsas the bioremoval reached 57.0 ± 3.4 and 21.6 ± 2.5, respectively.Bioremoved lead ions were recovered (P<0.05) using three different washing solutions, 0.1M EDTA was the most efficient since it recovered about 63.9 ± 5.6 mg/ml, while Na2CO3 was able to recover 35.9 ± 2.6 mg/ml; whereas 0.1M HCl recovered 21.8 ± 2.6 mg/ml. genomic DNA was extracted from different isolates of K. pneumoniae in order to study the relationship between genotype using BOX-PCR and bioremoval of lead. Three clusters were detected C1, C2, and C3 with similarity percentages reached 52, 60, and 100%. What's more, bioremoval did not depend on particular genotype
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Main Subjects
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