Molecular Heterogeneity of the Growth Hormone Gene and the Study of some Hormonal Changes for Individuals with Growth Hormone Deficiency

A. Rafee, Tamadir; M. Rasheed   N, Rasheed; A. Sulaiman, Ahmed; K. Al-Amee, Safaa

doi:10.37652/juaps.2015.127620

Document Type : Research Paper

Authors

¹ Presidency of Anbar University

² University of Anbar - College of Science

10.37652/juaps.2015.127620

Abstract

This study was conducted to measure a group of hormones related with infected individuals with the shortage of the growth hormone by studying 150 patients in comparison with 50 samples taken from normal individuals as a control group. The study included investigation GH gene via polymerase chain reaction technology (PCR) by two primers designed specifically for this study (GH3, GH4). The results showed that mutations occurred in 12 individuals from the samples, 8% for GH3 , whereas the GH4 showed no absence or disorder in the sequence of bases. The results of Sequencing using the Blast program showed appearance of many mutations of deletion mutations and insertion mutations , and substitution mutations of two types of transition and transversion. The concentration of hormones has been measured were human Testosterone hormone , Prolactin hormone ,Cortisol hormone ,The results showed a highly significant differences in the levels of the averages of hormonal analysis , as well as a significant decrease in the average length of individuals and high moral appeared in body mass index(BMI) under the moral under the moral level of P ≤ 0.05.

Main Subjects

Biology

References

1-Danna, K. and Jacob, P. (2012). Human growth hormone a life-changer for growth deficient children. Northwestern University. Retrieved .

2-Cohen, P., Germak, J., Rogol, A.D., Weng, W., Kappelgaard, A.M. and Rosenfeld, R.G. (2010).Variable degree of growth hormone (GH) and insulin-like growth factor (IGF)sensitivity in children with idiopathic short stature compared with GH-deficientpatients; evidence from an IGF-based dosing study of short children. J ClinEndocrinolMetab. 95: 2089-2098.

3-Shariat, N. Holladay, C. D. Cleary, R. K. Phillips, J. A. Patton, J. G.(2008). Isolated growth hormone deficiency type II caused by a point mutation that alters both splice site strength and splicing enhancer function. Clin. Genet. 74: 539-545.

4-Brooks, A.J., Wooh, J.W., Tunny, K.A. and Waters, M.J. (2008). Growth hormone receptor; mechanism of action. Int J Biochem Cell Biol 40: 1984-1989.

5-Storr. H., Chan, L., Grossman, A. and Savage, M. (2015). Paediatric Cushing's syndrome; epidemiology, investigation an therapeutic advances. Trends EndocrinolMetab; 18: 167.

6-Babu, D., Mellone, S., Fusco, I., Petri, A., Walker, G.E., Bellone, S., Prodam, F., Momigliano-Richiardi, P., Bona, G. and Giordano, M.(2014).Novel mutations in the GH gene (GH1) uncover putative splicing regulatory elements. Endocrinology. 155: 1786-92.

7Abraham, R., Sara, A., Athalia, P., Zvi, Z., Zvi, L.(1980).Prolactin secretion in 70 patients with growth hormone deficiency.European Journal of Pediatrics. 3: 251-258

8-Galdiero, M., Pivonello, R., Grasso, L.F. and Colao, A. (2012).Growth hormone, prolactin, and sexuality.J Endocrinol Invest. 35: 782-94.

9-Maria, G. F., Osorio, S., Aleander, A. L., Jorge, A. C. and Latronco, A. (2002). Pituitary Magnetic Resonance Imaging and Function in Patients with Growth Hormone Deficiency with and without Mutations inGHRH-R, GH-1,orPROP-1Genes . The Journal of Clinical Endocrinology & Metabolism 87: 5076 –5084.

10-Hess, O., Khayat, M., Hwa, V., Heath, K.E, Teitler, A. and Hritan, Y. (2013 ). A novel mutation in IGFALS, c.380T>C (p.L127P), associated with short stature, delayed puberty, osteopenia and hyperinsulinaemia in two siblings; insights into the roles of insulin growth factor-1 (IGF1).ClinEndocrinol (Oxf). 79: 838-844.

11-Misra, M., Bredella, M.A., Tsai, P., Mendes, N. and Miller, K.K . (2008). Lower growth hormone and higher cortisol are associated with greater visceral adiposity, intramyocellular lipids, and insulin resistance in overweight girls. Am J PhysiolEndocrinolMetab 295: E385–E392.

12-Isaksson, O.G, Jansson, J.O. and Gause, I.A. (2002). Growth hormone stimulates longitudinal growth directlyScience. 216:1237-1239.

13-Leung, K.C., Johannsson, G., Leong, G.M. and Ho, K.K. (2004). Estrogen regulation of growthhormone action. Endocr Rev 25: `1693-721

14-Lifshits, F. and Moses, N. (1988). Nutritional dwarfing;Growth, dieting and fear of obesity. J Am collNutr 7: 367.

15-Mukherjee, A., Ryder, W.D, Jöstel, A. and Shalet, S.M. (2006).Prolactin deficiency is independently associated with reduced insulin-like growth factor I status in severely growth hormone-deficient adults.J ClinEndocrinolMetab. 91: 2520-2525.

Journal of University of Anbar for Pure Science

Molecular Heterogeneity of the Growth Hormone Gene and the Study of some Hormonal Changes for Individuals with Growth Hormone Deficiency

References

References

Volume 9, Issue 3
December 2015
Page 114-120

Molecular Heterogeneity of the Growth Hormone Gene and the Study of some Hormonal Changes for Individuals with Growth Hormone Deficiency

References

References

Volume 9, Issue 3December 2015Page 114-120

Volume 9, Issue 3
December 2015
Page 114-120