Document Type : Research Paper

Authors

University of Anbar / College of Science

10.37652/juaps.2015.127623

Abstract

This study was carried out to isolated and diagnoses E. Coli bacteria case infection of the urinary tract also study virulence factor Haemolysin A in the isolated more than resistance to antibiotic. This aim of the study 200 sample of urinc were collected from patients in Ramadi teaching hospital (male and female) of different age (one year – 70 years). the E. Coli bacteria were the most spreading type in the case of urinary tract infection since they were isolated with percentage 59% out the total number of bacterial isolates, indentification the disc diffusion method was used test the isolate towards (10) ten antibiotics this work also includes the study of some virulenece factor of isolated bacteria. The result revealted that a high ratio of bacteria which are gram negative E. Coli isolated from urinary tract infection showed high resistance to the antibiotic particularly cefotaxim 100%. Naldic acid 95.3% Gentimicin 97.2%. ten isolatedwere chosen from each bacteria types for the sake of studying their genetics homogeneity. DNA was extracted from ten isolated of each of the following bacteria E. coli this extracted DNA was used in multiplex (PCR) technique by using gen of Haemolysin. A. This result found one coloney this carries the gen (Hyl A_ number (7) to DNA genome sample. While the other result fond (Hyl A) for plasmed DNA in the samples (3 -6- 7).

Keywords

Main Subjects

  1. Stapleton,Ann(2005).Novel  Mechanism of   p- Fimbriated  E. coli Virulence in Pyelonephritis.J.am.soc. nephrol. 16:3458-3460.
  2. Al – Begat , Saad Taha Mutlk Hmidon ( 2007 ). Study of most commen aerobic  bacteria causing lower urinary tract infection (UTI) in Ramadi general hospital. thesis , college of medicine – university of Al – Anbar.
  3. Emody ,I, Kereny  M. and Nagy  G.(2003).Virulance factor of uropathogenic E. coli. antimicrob. agents. 22 (suppl 2) : 29 – 33
  4. Brooks, g, butel, j and morse, s.(2007). jawetz , melnick and ad elberg's  medical microbiology , 4th ed. macGraw hill co. Newyork : 151-152.
  5. Nioclle, L.E. (feb 2008) Uncomplicated Urinary tract infection in adults including uncomplicated pyelonephritis. Urologic elinics of north America. 35 (1)
  6. Hooten,T.M. (2000).Pathogenesis of urinary tract infection: anUpdate. J. Antimicro. Chemo. 45, (Sapplment) : 1-7
  7. Gary , C.F.(2006 ). Urinary tract infection during pregnancy. American Academy of family physicians. Williams obstetrics 22 ed. Ch. 48.
  8. Kadir , Dr.Mohammed , Majida   N. lbrahim  and  Najeeba M. Salih (2010).Prevalence of Urinary Tract Infections in Patients with Renal stones.Kirkuk
  9. Magdy m. Afifi.  (2013). D etection of extended spectrum Beta- lactamase  producing Klebsiella pneumonia  and Escherichia  coli  of environmental surfaces at upper Egypt Jnternatiomal journal of Biological chemistry; 10:3923.
  10. Wult, B. G. Bergsten ,H. Connel ,P.R.OIIqno (2000).P. fimbriae enhance the early establishment of E. coli in the human urinary tract ,mol.microbiology ,38:456-464.
  11. Brooks , g, f , Butal , j.s. and Morse , S.A. ( 2001 ). " jawetz , melnick & abelbergs  medical microbiology ". 22nd ed , lange medical books / McGraw – Hill inc , U.S.A.
  12. Bartolon. A.i , L. Pallecchi, E. Riccobono , A. Mantella , D. Magnelli, T. Di Maggio , A. L. Villagran , Y. Lara, C. Saavedra ,M. Strohmeyer , F. Bartalesi , C. Trigoso and G. M. Rossolini. (2013). Relentless increase of resistance to fluoroquinolones and expandedspectrum cephalosporins in Escherichia coli: 20 years of surveillance in resource-limited settings from Latin America. J Clinical Microbiology and Infection; 19: 356-361.
  13. MAcfaddin, J.E. (2000) individual biochemical test. In: Biochemical test for identification of medical bacteria (3rded) Macfaddin J. E. (ed.) p:27-439. Lionicott Williams & wilkins Co. Blatimore. U.S.A.
  14. MartinaBielaszewska, Christian Rüter, Lisa Kunsmann, LiloGreune, Andreas Bauwens, Wenlan Zhang, Thorsten Kuczius, KwangSik Kim, Alexander Mellmann, M. Alexander Schmidt, HelgeKarchPLoSPathog. 2013.
  15. Koronakis V, Hughes C, 2002. Hemolysin. In Donnenberg MS (ed.), Escherichia coli: virulence mechanisms of a versatile pathogen. Academic Press. San Diego, Calif. pp. 361-378
  16. Al – khozai , Ziad M. ( 2009 ). Studying the adhesion properties of Pseudomonas aeruginosa and staphylococcus aureus on contact lenses and the effect of radiation with electrons and positrons on its adhesion in the laboratory , Journal of Karbala university. vol7 , no1:34- 39.