Document Type : Research Paper
Authors
University of Anbar - College of Science
Abstract
Identification of 66 bacterial isolates obtained from different sources has been shoud belonging to the bacterium P. aeruginosa in arate of 46 infection isolation and 20 environmental isolates which confirmed by Microscopy diagnosis and Biochemical tests.Micro titration plates method was (MTP) used for detection of isolates abilites to produce Pyocyanin dye. The isolates were differed in their production of Pyocyanin dye between productive with high efficiency, medium efficiency and low efficiency.For the study of gene expression of these isolates, extracted RNA was obtaind from of all isolates, then measure the amount of gene expression by qRT- PCR technology for gene PhzAresponsible for production of Pyocyanin dye, was studied the isolates incuded were 56 as treatment isolates and 10 as control isolates of each gene using the One Step Real time–PCR. It was found that the phzA gene expression by relative Quantification method ,within the range (0.93-2.12) ,with highly significant differences and probability (P<0.001).
Keywords
Main Subjects
- ألخالصی ، علی حسین علأ الدین. 2014)). دراسة تأثیر التثبیطی لعسل النحل المحلی ضد الإصابة البکتیریة واستعمالھ فی معالجة الجروح والحروق.کلیة التربیة الأساسیة ، الجامعة المستنصریة.
- المشهدانی, أحمد محمد یوسف یاسین. (2016).التباین المظهری والجزیئی لبعض الجینات الخاصة بالضراوة والصفات التشخیصیة للعزلات المرضیة للزوائف الزنجاریة , رسالة ماجستیر ,کلیة العلوم ,جامعة الانبار.
- النعیمی ,علا عبد الکریم کاظم. (2015). دراسة تأثیر البروبایوتک فی تکوین الغشاء الحیوی وانتاج أنزیم البروتیز لبکتریا Pseudomonas aeruginosa المعزولة من أخماج الحروق والجروح , رسالة ماجستیر,کلیة التربیة الأساسیة ,جامعة المستنصریة.
- Baron, E. J. ; Finegold, S. M. and Peterson, I. L. R. (2007).Bailey and Scotts Diagnostic Microbiology. 9th ed. Mosby Company. Missouri.
- Bin Ren, Huifeng Shen, Zhi John Lu, Haiming Liu , Yuquan Xu (2014). The phzA2-G2 Transcript Exhibits Direct RsmA-Mediated Activation in Pseudomonas aeruginosa M18. PLoS ONE; 9(2): 1-13.
- Brooks, G.F.; Carroll,K.C; Butel,J.S.; Mores,S.A. and Mietzner,T.A. (2010).Jawetz,Melnich andAdelbergs Medical Microbiology.25th ed.thr McGraw-Hill companies,United states of American.
- Can Attila. (2008). Identification of Pseudomonas aeruginosa Virulence Factors via a Poplar Tree Model. College of Science. Istanbul Technical University, Turkey.
- Chin-A-Woeng, T. (2000). Molecular basis of biocontrol of tomato foot and root rot by Pseudomonas chlororaphis strain PCL1391. PhD thesis, University of Leiden, Leiden, The Netherlands.
- Dumas, J.L.; Delden, C.V.; Perron, K. and Delden, T.K.(2006). Analysis of antibiotic resistance gene expression in Pseudomonas aeruginosa by quantitative real- time PCR. FEMS Microbiol. Lett.; 254:217-225.
- Jacoby, G.A. (2009). AmpC beta-lactamase. Clin. Microbiol Rev. 22(1): 161-182.
- Kaltenboeck B and Wang C (2005). Advances in real-time PCR: application to clinical laboratory diagnostics. Adv Clin Chem; 40:219–49.
- Lu, Y.Q.; Han, J.X.; Qi, P.; Xu, W.; Zu,Y.H. and Zhu, B. (2006). Rapid quantification of hepatitis B virus DNA by real-time PCR using efficient TaqMan probe and extraction of virus DNA. J. World J Gastroenterology. 12(45): 7365-7370.
- Mavroid, D. V. ; Bonsall, R. F. ; Delaney, S. M. ; Soule, M. J. ; Phillips, G. and Thomashow, L. S. (2001). Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1carboxamide from Pseudomonas aeruginosa PAO1. J. Bacteriol.; 183(21): 6454-6465.
- Nidhi Shah, NatashaKlaponski, CarrieSelin , Rachel Rudney,W.G.Dilantha Fernando, Mark F.Belmonte and TeresaR.deKievit. (2016). PtrA Is Functionally Intertwined with GacS in Regulating the Biocontrol Activity of Pseudomonas chlororaphis PA23;7(1512):1-13.
- Pierce, K.E.; Rice, J.E.; Sanchez, J.A.; Brenner, C. and Wangh, L.J. (2000). Real-Time PCR using molecular beacons for accurate detection of the Y chromosome in single human blastomeres. J. molecular human reproduction; 6(12):1155-1164.
- Rada B, Lekstrom K, Damian S, Dupuy C, Leto TL (2008). The Pseudomonas toxin pyocyanin inhibits the dual oxidase-based antimicrobial system as it imposes oxidative stress on airway epithelial cells. The journal of immunology.; 181: 4883-4893.
- Rastegar, L.A.R.; Alaghehbandan, R. and Akhlaghi, L. (2005). Burn wound infections and antimicrobial resistance in tehran, Iran:an increasing problem. Annals of Burns and Fire Disasters; 453(2): 1-9.
- Zubair, M.; Malik, A.; Ahmad, J.; Rizvi, M.; Farooqui, K.; Rizvi, M. (2011). A study of biofilm production by gram-negative organisms isolated from diabetic foot ulcer patients. J. Bio. Med.; 3(2): 147-157.
)
