Document Type : Research Paper
Authors
1 University of Anbar - Collage of Education for Women
2 University of Anbar - Collage of Medicine
3 Al-Mustansria University - National centre of Hematology
Abstract
This study was carried out during the period from March 2016 to January 2017 to detect of genotypes and subgenotypes of Giardia lamblia in human feces by PCR-Restriction fragment length polymorphism based on the detection of glutamate dehydrogenase gene and study the corralation between genotype and prescence of symptoms , a total of 101 stool samples were taken from patients(male and female), aged(2-72) years, who suffering from acute or persistent diarrhea and examined by microscope and molecular techniques , the gdh gene was amplified from 19 cases only by a conventional PCR assay by using a specific oligonucleotide sequence for coding region by gdh gene at size 432bp and typed by RFLP analysis, the results showed the total infection with Giardia lamblia was 84(83.1%) according to the results of microscopic examination, of the 19 positive samples, 2(1.98%) were infected with genotype AI , 3(2.97%) genotype AII , 5(4.95%) genotype BIII and 9(8.91%) infected with genotype BIV, with significance differences between the genotype and precence of symptoms
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- Abebe, A. ; Daniels, J. ; Mckean, J.W. ; Kapenga, J.A.(2000). Statistics and Data analysis, university of Oxford.
- Algazoui, R.M.A. ; Lgreed,M.A.O. ; Abdulkader, F.M.(2016). Prevalence and clinical symptoms of giardiasis among children in sebha city, I.J. of Appl. Med. Bio. Res.; 1(1):12-19.
- Al-Saeed, A.T. ; Issa, S.H.(2006). Frequency of Giardia lamblia among children in Dohuk, Northern Iraq. Eastern Mediterranean Health J. ; 12(5):555-561.
- Amar, C.F.L. ; Dear,P.H. ; Mclauchlin, J.(2003). Detection and genotyping by Real-time PCR-RFLP analysis of Giardia duodenalis from human faces. J. med. Micro. ; 52: 681-683.
- Atherton, R. ; Bhavnani, D. ; Calvopina, M. ; Vicuna, Y ; Cevallos, W. ; Eisenberg, J. (2013). Molecular identification of Giardia duodenalis in Ecuador by polymerase chain reaction – restriction fragment length polymorphism. Mem. Inst. Oswaldo. Cruz., Rio de janeiro, 108(4):512-515
- Bertrand,I.; Albertini,L.; Schwarlzbrod,J.(2005). Comparsion of two target genes for detection and genotyping of Giardia lamblia in human feces by PCR and PCR-Restriction fragment length polymorphism, journal of clinical microbiology, 43(12): 5940-5944.
Brooke ,M.M. ; Melvin,D.M.(2001). Morphology of diagnostic stages of intestinal parasites of human. U.S. department of health and human services, Second Edition. - Cardoso, E.J. ; Garcia, L.E. ; Campos , A.C. ; Estrada , A.C. (2012).Genotyping of Giardia intestinalis isolated from Dogs by analysis of gdh , tpi and bg genes . Available from:http://www.intechopen.com/books/parasitology/genotyping-of-giardia-intestinalis-isolates-from-dogs-by analysis-of-gdh-tpi-and-b-giardin-genes.
- Degerli, S. ; Degerli, N. ; Celiksoz,A. ; Ozcelik, S.(2012). Genotyping of Giardia intestinalis isolated from people living in Sivas, Turkey. Turk. J. med., sci., 42(1): 1268-1272.
- Dryden,M.W. ; Payne, P.A. ; Ridley,R. ; Smith,V.(2005). Comparison of common fecal flotation techniques for the recovery of parasite Eggs and Oocysts. Veterinary therapeutics ; 6(1):361-370.
- Hazrati-Tappeh,k. ; Manafi,G. ; Asgharzadeh,M. ; Manafi,F.(2014). Incidence of Giardia lamblia Subspecies by PCR-RFLP in stool specimens of Hospitalized children at Urmia Mutahhari Hospital, west Azerbaijan province, Iran.Iranian J Parasitol ; 9(4):541-547.
- Joshi,M. ; Deshpande J.D.(2011). Polymerase chain reaction : Methods , principles and application. International Journal of Biomedical Research; 1(5): 81-97.
- Kumar,S. ; Singh,V.A.(2016). Prevalence of Entamoeba histolytica and Giardia lamblia infection in a rural area of Haryana, India. Int. j. curr. Micro. App. Sci. ; 5(6):204-209.
- Mbae, C. ; Mulinge,E. ; Guleid, F. ; Wainaina, J. ; Waruru, A. ; Njiru, Z.K. ; Kariuki,S.(2016). Molecular characterization of Giardia duodenalis in children in Kenya. BMC. Infectious diseases; 16:1-7.
- Molina,N.; Minvielle,M. ; Grenovero,S.; Salomon, C. ; Basualdo,J.(2011). High prevalences of infections with Giardia intestinalis genotype B among children in urban and rural areas of Argentina. Annals of tropical medicine and parasitology ; 105(4):299-309.
- Nahavandi, K.H.; Fallah,E.; Asgharzadeh,M.; Mirsamadi,N.; Mahdaripour,B.(2011). Glutamate dehydrogenase and triosephosphate isomerase coding genes for detection and genetic characterization of Giardia lamblia in human feces by PCR and PCR-RFLP, Turk. J. Med. Sci., 41(2): 283-289.
- Narla, N.P. (2010). Allelic heterozygosity within and among Giardia lamblia genotypes B isolates. Thesis. Department of medicine and immunological, university of Arizona.
- Pestechian, N.; Resekh,H.; Nejad,M.R.;Yousifi, H.A.; Safa,A.H.(2014). Molecular identification of Giardia lamblia ; is there any correlation between and genotyping in Iranian population. Gastroenterol hepatol Bed. Bench, 7(3): 168-172.
- Qader, A.M. ; Bakir , T.Y.(2011). Molecular identification of Giardia duodenalis parasite isolates from human by polymerase chain Reaction _ Restriction Fragment length polymorphism Technique (PCR_RFLP) in Baghdad province. Diyala Jou. Pub. Sc. 7(4): 54-66.
- Roointan,E.S. ; Rafiei,A. ; Samarbaf-Zadeh,A.R.; Shayesteh, A.A. ; Shamsizadeh,A. ; Borujeni, M.P.(2013). Genotype analysis of Giardia lamblia isolated from children in Ahvaz,South west of Iran.Jundishapur J. micro. ; 6(3):279-283.
- Sarkari, B. ; Ashrafmansori, A. ; Hatam, G.R.; Motazedian, M.H.; Asgari, Q.; Mohammadpour, I. (2012). Genotyping of Giardia lamblia isolates from human in Southern Iran. Trop. Biomed ; 29(3):366-371.
- Zheng, G. ; Al-sarakibi, M. ; Liu, Y. ; Hu, W.; Luo, Q. ; Tan, L. ; Li, G.(2014). Genotyping of Giardia duodenalis isolated from dogs in Guangdong, China based on multilocus sequence. Korean J. parasite. ; 52(3): 299-304.
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