Document Type : Research Paper

Author

Anbar University - College of Science

10.37652/juaps.2010.15275

Abstract

:The present study sheds light on knowing the spread of klibsilla oxytoca Bacteria in
(100) soil sample taken from the regions of AL-Jazeera ,AL-Taameem, AL-Khaldiya , AL-Sufiya and
AL-Habbaniya .The ratio of the existence of these Bacteria ranges between 12-88% . The results
indicate that all samples contain klibsilla oxytoca Bacteria ;and (36) isolate has been obtained form
these Bacteria . The study also includes the evaluation of the effeiency of isolates in foxing Nitrogen .It
is appeared that the highest quantity of foxing Nitrogen reaches at (1.3) mlg Nitrogen / 100 ml . by
local isolation klibsilla oxytoca J3 .When studying the effect of different concentrations of NP and
Urea fertilizers on the growth of isolate in the solid and liquid cultures from the vrem point of colonies
diameters and numerical density, the result show the ability of this isolate on the growth at the addition
of a quarterly concentration (83 and3.2) per million to NP fertilizer and Urea fertilizer respectively . the
diameter .Colonies that grow in the solid culture is ( 2,2,5,3,2,3,4,4,and 3) ml ,respect rely ,during the
periods g incubation (144,120,96,72,48,and 24)hour . While the nureoced density reaches at
(9.21,9.23,8.98,8.74,7.93,6.98)Log/ml .The results eg .Biology experiment proved the efficiency of this
isolate in foxing Nitrogen . Appeared in all the features g plant , (plant weight ,plant length , plant
content of nitrogen and chlorophyll ) ,that are studded in the treatment in which isolate mixture is used
with quarter of concentration (3.2, 83) per million to NP fertilizer and Urea fertilizer respectively . The
results state that the mixture of isolate with a quarter of recommendation is able to foxing Nitrogen at a
rate of 4.43 mlg Nitrogen / plant . while the soil content of total Nitrogen is 120.63 mlg/kg and the 8.09
microbial density Log/g soil . It is found that the best rote to these plant ,in this treatment reaches (20 ,
74 )in the plant bight and rote con respectively and the dry weight reaches at ( 178 ,16.2 ) mg /plant

Keywords

Main Subjects

1- Green wood,D.; Slack : R.C.B.; and peutherer ,J.F.(1997)Medical microbiology: Aguide to microbiol. Infection : Pathogensis immunity, Laboratory Diagonsis and control .15th ed .
2- Liu-J.; Liu,c.; and lin, c.(1997). The role of Nitrogenase in acyanid -degrading klebsilla oxytoca strin. proce.Nation scien. Councl., 21(2) : 37-42 .
3- Holt ,J.G.; Krieg ,N.R.; Sneath ,P.H.A.;Staley, J.T.; and Williams ,S.T. (1994). Bergey’s manual of Determinative Bacteriology.
4- Cruickshank,R.;Duguid,J.P.; Marmiom, B.P.; and swain, R.H.A. (1975) . Medical Microbiology: The practice of medical microbiology. 12th .ed., vol 2 churchill Living ston, Edinburgh, London and Newyork.
5-Bracker, G.; Fesefeldt, A.; and Witzel, K. (1998). Development of PCR primer system for amplification of Nitrata Reductase Genes (nirk and nirs) to detect Denitrifying Bacteria in Environmental samples, App.and Envir . Microbiol., 64 ( 10): 3769- 3775 .
6- Mark Doolittle ,Ashok Raina, Alan Lax and Baj Boopathy (2008). Presence of nifrogen fixing klebsiella pneumoniae in the gut of the for mosan subterranean termite (coptotermes formosanus) Bioresource Technology 99: 3297- 3300.
7- Ronkko, R.; Smolander, A.; Nurmiaho- Lassila ,EL.; and Heahtla ,k. (1993). Frankia in the rhizosphere of nonhost plant : Acomparsion with root–associated N2– Fixing Enterobcter, klebsiella and pseudomonas . plant and soil ., 153: 85 – 95.
8 – Y. Ding, J. Wang ,Liu and S. Chen .(2005). Isolation and identifcation of nitrogen fixing bacilli from plant rhizospheres in Beijing region . Joumal of Applied Microbiology, ( 1271- 1281) .
9- Podschun, R.; Ullman ,U (2009) . Klebsiella spp as Nosocomial pathogens : Taxonomy , Typing methods, and clinical microbiology Reviews 11(4):589-603.
10- Tate, R.L.( 1995). Soil microbiology : Johnwily and sons . Inc . Newyork .
11- F.C. Cannon & J.R. Postgate.(2010) Expression of klebsiella nitrogen fixation gens (nif) in Azotobacter .Journal Home current lssue Aop Archive (260- 272).
12 – Artur k. Zlothikov, Yelena N. Shapovalova and Alexei A. Makarov (2001). Association of Bacillus furmus E3 and klebsiella terrigena E6 with increased ability for nitrogen fixation. Soil Biology and Biochemistry: 33(1525- 1530).
13- Baron ,E.F.; and Fingold, S.M.( 1990).Baily and scott diagnostic microbiology C.V. mosby company,st . ious .Batihore , philadelphia tornoto.
14- Maynes, R.J.A. (1980). Companism of two modifical kgeldale olcyestion techniqa for mutti- element Gmmunceotion in soil science & plant Analsis II :   459 – 467 .
15- Louw, H.A & Webley, D.M.(1985). Plate method for estimatig the numbers of phosphate dissolving and acid productivity bacteria in soil natur Lond .182:1317-1318
16– Le-van ,N;A. Lewan, Gt; Nideman, T.S; Trueb, W; Boeisterterli, JJ. And sanglier, JJ. (1992). Anoval class of axs intibtors cuonin A and B form strepto. Spp. Cited by sanglier  jj . Haag, H., Huck , T. A and Fehr,T. (1992). Res . Microbial.
17 –Venkateswarlu, B.V. Rao & P. Raina. (1984). Evalution of phosphorus solubilization by microorgansim islated form aridisolis.J. Indian .Soc .Soil Sci . : 273- 277.
18 – Vora ,M.S. & Shelat , H.N .( 1999) . Impact of addition of different carbon & nitrogen sources on solubilizing of rock phosphate by phosphate solubilizing of microorganisms , Indian J .Of Agri .Sciences V. 68 (6) : 292 – 294 .
19 – Haller, T.& Stople, R.H.(1985). Quantitative estimation of root exudation of maize plant & soil., 86: 207- 216 .
20- الظفیری, محمد ابراهیم (1999). تاثیر مستوى الکاربون فی    المواد العضویة المضافة والتلقیح بـ Azotobacter Vinelandii فی تغیر التربة. رسالة ماجستیر, کلیة الزراعة, جامعة بغداد.
21– Santck, B.& Maric , V.( 1995) .Temperatur & dissolved oxygen concentrions as parameters of Azotobacter chroococcm ,cultivation for use in biofertilizers .Biotechnol .Lett . London ,UK :chopmam & Hall V.17 (4 ) : 453 – 458
22–Subba- Rao, N.S. ( 1982). Phosphate solubilizing by soli microorganisms in advancing agricultural microbiology. Ed. Butter worth. Scientific .London Bston . Singapore. Toronto : 205 – 303 .
23– Cordvilla , MP.Ligero , F.Liuch ,C.(1995). The effect of salinity on N-fixation & assimilation in viciafaba. J. Exp. Bot Oxford .45(279): 1483- 1488.
24– العیثاوی, احمد محمد ترکی (2007). دراسة دور البلازمیدات فی تثبیت النتروجین فی بکتریا KL.Pneumoniae وتحدید کفاءتها فی التثبیت بایولوجیا. مجلة جامعة الانبار للعلوم الصرفة, العدد الاول, المجلد الاول: 15-23.
25– العسافی, ادهام علی , احمد محمد العیثاوی وظافر فخری الراوی (2008). استخدام العزلة Azotobacter vinelandii المحورة وراثیا مع تحضیر سماد عضوی حیوی من مواد محلیة واختباره حیویا. مجلة الانبار للعلوم الزراعیة, مجلد: 6 العدد (2): 275-278.
26–Tomar, R. Raghu, J. Yadaf, L &  Ghuraya ,R.S.(1992) . Effect of phosphorus ,Rhizobioum inoculation & Zinc on the Yeild of soybean (Glycine max .L) int .J. Trop Agricult .9 (3): 211- 214.
 
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