Document Type : Research Paper

Authors

Department of Life Sciences - College of Science - University of Anbar

Abstract

An activity of azadirachtin compound after separating it from
Melia azedarach fruits powder and purification as well as diagnosing it by
IR,CC,TLC, against some pathogenic bacterial isolations which are
Staphylococcus aureus , Proteus , Pseudomonas aeruginosa, Escherichia coli ,
has been studied .This compound has proved its high activity to influence against
these bacterial isolations and inhibated the diameter of inhibation it was
23,18,17,22mm with concentration 50mg/ml respectively . An activity of hexane
extract of Melia azedarach fruits powder also has been studied to prove its good
activity against same bacterial isolations but in diverse rates between it and
azadirachtin compound extract since diameters of inhibition zone have reached
13,19,21,12 mm with concentration 50 mg/ml respectively

Main Subjects

 
[1] قریشی, م. سعید. (1990). المکافحة الکیمیوحیویة وتأثیراتها على الاقتصاد والبیئة والانتخاب الطبیعی. (ترجمة) هانی جهاد العطار. مطبعة جامعة الموصل. 363 صفحة.
[2] درویش، مصطفى. (1984). موجز علم العقاقیر الطبیة لطلاب معاهد المهن الصحیة العالمیة فی العراق. المکتبة الوطنیة. بغداد.
[3] خلیل، یاسین. (1979). الطب والصیدلة عند العرب. مطبعة جامعة بغداد.
 [4] الشحات، نصر أبو زید. (2000). النباتات والأعشاب الطبیة. الدار البحار للنشر والتوزیع. بیروت.
[5] Broughton, H.B.; Jones. P.S. Ley, S.V.; Morgan, E.D.; Slawin, A.M.Z. and Willimes, D.J. (1986). The chemical structure of azadirachtin. proc. 3rd .Int. Neem Conf., Nairobi. H.Schmutterer and K.R.S. Ascer, (eds).pp103-110.
[6] NIST, (1998). National Institute Of Standerds Technology, Mass Spectrometry Data Base Center 1A, USA.
[7] Baron, E.J; and Finegold, S.M. and Baily Scott. (1990). Diagnostic    Microbiology; C.V mosby company Toronto. 
[8]Cruickshank, R. Daguid, j,marmion and Swain ,R.(1975). Medical microbiology   12ed churrchill living stone London.
[9] جاداللة. نزار فؤاد، العزام عقاب، الشاعر. عبد المجید، المنسی عرسان (1994) الأحیاء الدقیقة العملیة. سلسلة الطرائق الأساسیة، عمان.
[10] Yamasaki, R.B.; Kloke, J.A.; Lee, S.M.; Stone, G.A. and Darlington, M.V. (1986). Isolation and purification of azadirachtin from neem (Azadirachta indica) seeds using flash chromatography and high – performance liquid chromatograph.J.Chromatog. 356: 220-226.
[11] Egorov, N.S.(1985)Antibiotics scientific approach mir publisher ,Moscow.
[12] Harbone, J. B. (1984): Phytochemical methods A Guid to modern techniques of plant analysis. Chapman & Hall. New York.
[13] Valladares, G.M.T. Defago, S. Palacios, and M.C. Carpinella. (1997). Laboratory evaluation of Melia azedarach L. (Meliaceae) extracts against the elm leaf beetle (Coleoptera: Chrysomelidae). J.Econ. Entomol. 90(3):747-750.
[14] Hoit, J.H., Krieg, N. R.sneath, P.H.A., staleg, J.T., and Williams, S.T. (1994). Bergeys manual of determinative bacteriology9th .ed U.S.A.
[15] Butterworth, J.H. and Morgan, E.D. (1971). Investigation of the locust feeding inhibition of the seed of the neem tree Azadirachta indica. J. Insect physiol. 17:969 -977.
[16] Chandler, L.D.(1988). Insect growth regulators and plant extracts for control of leafminer .Journal Rio Grande Valley Horticulture Society 39: 75-78.
[17] Rembold, H. (1989).Azadirachtins: their structure and mode of action. In: Insecticides of plant origin, ACS symp.ser.387 (eds.Arnason, J.T., philogene, B.J.R. and Morand,P.) pp.:150-163. American chemical society, Washington, DC.
[18] صالح .ضحى سعد (لجنه من تدریسی قسم علوم الحیاة) (1991) علم الأحیاء المجهریة .دار الحکمة. جامعه بغداد العراق ص391.
[19] Wolters, B. (1976). Serial experiments on transformation and degradation of secondary plant substances by microorganisms. Plant Medica, 29:41-53.