Document Type : Research Paper

Authors

Anbar University - College of Science

10.37652/juaps.2009.15345

Abstract

The spectrophotomatric spectra in UV, Visible and IR regions have revealed the
presence of many functional groups in the aqueous and ethanolic extracts of Hibiscus sabdariffa L.
flowers .Paper, thin-layer and column chromatographic techniques have shown the presence of three
pigments in both extracts ; Gossypetin, Anthocynin, and Querectin .Five organic acids were also
separated and identified in both extracts .All these compounds were identified by UV, Visible and IR
spectrophotomatry of electromagnetic radiation .Flame atomic absorption and emission technique
haveoffered high levels of determination of calcium, barium, potassium and magnesium, while sodium,
lithium, iron, manganese, copper, zinc and nickel were found in traces .pH and potential titrations of
the extracts with standardized NaOH have shown high inflections at equivalence points which were
exploited in the determination of total acids in the extract which was more than 28% .The sudden
colour change from violet, red or pinkish of the extract into green has led us to exploit the extracts as
an acid-base indicator . The data and results obtained have clarified that the extracts of Hibiscus
sabdariffa L. flowers are matching the action of other indicators used in acid-base titrations

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