Document Type : Research Paper
Author
Department of Life Sciences - College of Education - University of Anbar
Abstract
The study aimed at isolation starch hydrolyses bacteria and amylaseproducing
bacteria from Ramadi soils to get efficient local isolates that produce the
enzyme .30 bacteria isolates that produce the enzyme could be obtained; the (10) most
efficient of which were selected. Cultural media containing plant wastes prevailing in the
local environment were used. There are considered pollutants that are very cheap in the
production of the enzyme. They are corn cap, clover hay Trifolium alexandrinum L., and
shamblan Ceratophyllum demersum L. . The results have shown that the best production
of the enzyme using starch agar was at pH7 and 35c temperature after 24 hours of
incubation with the use of corn cap then shamblan as a source of carbon where the
diameter of the clear zone area was 8cm from the local isolate (PR10). The local isolate
(PR10) showed high ability to produce amylase enzyme using liquid cultures. The best
production was at pH7 and 40c temperature using corn cap of2% concentration and
incubation with the shaking incubator at 150 rpm and 1.5% inoculums concentration.
The activity of the enzyme was 24.731 unit/ml using peptone as a source of nitrogen after
incubation for 24 hours. Identification results of (PR10) isolate showed that it is B.subtilis
bacteria.
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