Document Type : Research Paper

Authors

1 Mosul University - College of sciences

2 Mosul University - College of Education,

10.37652/juaps.2009.15439

Abstract

The enrich methanolic extract of unripe fruit of Melia azedarch L. which was given with the food of
larvae beet armyworm, Spodoptera exigua Hunber.Concentrations of the extract which were ranged between 0.25 to
10.0 ppm were used to investigate the effect of M. azedarach extract on the gonads of adults derived from treated
larvae. Histological bases of the extract application were investigated inovaries and testes sections. Low
concentrations induced reduction of one ovary, or parts of the ovariole , or parts of the ovary were histolysed and
disappeared. Application of male larvae with 0.25 ppm of the extract caused separation of scrotum from fat bodies.
Gradual histological variation synchronized with the increasing extract concentration was as follows; reduction of
epithelial layer of testicular follicles and scattering of spermatogonia. High concentrations led to histolyse parts of the
scrotum, follicle envelop and fat bodies content

Keywords

Main Subjects

1.  Rathore, H. R., Tariq, G., Mujtaba, S. M. and Nasir, S. M. (1986). Insecticide resistance in Anopheline mosquitoes in Punjab province. Pakistan. J. Zool. 17 (1) : 35-40.
2.  Altstein, M., Aharonson, N. and Menn, J. J. (1993). New targets for insect management in crop protection. Arch. Insect Biochem. Physiol. 22 : 5 – 12.
3.  Hoffman, K. K. H. and Lorenz, M. W. (1998). Recent advances in hormones in insect pest control. Phytoparasitica 26 : 323 – 330.
4.  Wiltshire, E. P (1957). The lepidoptera of Iraq. Adlard and Son, Bartholomew press, Dorking.
5. Feder, D., Valle, D., Rembold, H. and Grarcia, E. S. (1988). Azadirachtin – induced sterilization in mature females of Rhodnius prolixus. Z. Naturforsch. 43 c : 908 – 913.
6.  Jeong, S. E., Lee, Y., Hwang, J. H. and Knipple, D. C. (2001). Effects of the sap of the common oleander Nerium indicum (Apocyanaceae) on male fertility and spermatogenesis in the oriental tobacco budworm Helicoverpa assulata (Lepidoptera : Noctuidae). J. Experimental Biol. 204 : 3935 – 3942.
7. Huang, L. C., Zhou, J., Suenaga, H., Takezaki, K., Tadera, K. and Nakatani, M. (1995). Insect andiffeding property of limonoids from okinauwa and chinese Melia azedarach L., and from Melia toosendan (Meliaceae). Biosci. Biotech. Biochem. 59 (9) : 1755 – 1757.
8. Connolly, J. D. and Hill, R. A. (2001). Triterpenoids. Nat. Prod. Rep. 18 : 560 – 578.
9. Bodhade, S. N. and Borle, M. M. (1985). Sterility effect of some indigenous plant material on cucurbit fruit–fly pp. 38 – 46. In Regupathy, A., Jayaraj, S. J. (ed.) Behavioueal and physilogrcal approaches in pest management. Coimbaloro, Tamil Nado, India.
10. Chiu, S. F., Huange, B. G., Hu, M – Y. (1986). The ovipostion deterring effect of some meliaceous plant extracts against the rice gall midge Orseolia oryzea, Wood Mason. Acta Entomol. Sin. 29 : 221 – 224.
11.  الحمدانی، منیف عبد (2002). تاثیر بعض منتجات النیم Azadiracha indica A. Juss ومستخلص ثمار السبحبح Melia  azedarach L. فی بعض الجوانب الحیاتیة والفسلجیة لدودة البنجر السکری (Noctuidae: Lepidoptera) Spodoptera exigua (Hub.) اطروحة دکتوراه، کلیة العلوم، جامعة الموصل.
12. Schmidt, G. H., Ahmed, A. A. I. And Breuer, M. (1997). Effect of  Melia azedarach extract on larval development and reprodution  parameters of Spodoptera littoralis (Boisd.) and Agrotis ipsilon (Hufn.) (Lep., Noctuidae). Anz. Schadlinskde., Pflanzenschutz., Umweltschutze 70 : 4 – 12.
13. Schmidt, G. H., Rembold, H., Ahmed, A. A. I. And Breuer, M. (1998). Effect of Melia azedarach fruit on juvinile hormone titer and protein content in the hemolymph of two species of noctuid lepidopteran larvae (Insecta : Lepidoptera : Noctuidae). Pytoparasitica 26 (4) : 283 – 299.
14. Breuer, M. and Deloof, A. (2000). Efficacy of an enriched Melia azedaeach L. fruit extract for insect control. Practice Orirnted Result on Use and Production of Neem Ingredients and Pheromones VI H. Kleeberg and C. P. W. Zebitz (eds.) Copyright 2000 by Druck and Graphic. Giessen, 173 – 183.
15. Ewen, A. B. (1962). An improved aldehyde fuchsin staining technique for neurosecretory products in insect. Trans. Amer. Micros. Soc. 81 : 94 – 96.
16.  کورکیس، نجم شلیمون (1996). دراسة طبیعیة وتجریبیة عن الترکیب النسیجی للمبیض وعملیة تکوین المح فی البعوضة الذاتیة التولد Culex pipiens molestus. رسالة دکتوراه، کلیة العلوم، جامعة الموصل.
17. Perveen, F. and Miyata, T. (2000). Effects of sublethal doses of chlorfluazuron on ovarian developmeent and oogenesis in the common cutworm Spodoptera litura (Lepidoptera : Noctuidae(, Ann. Entomol. Soc. Am. 93 : 1131 – 1137.
18. Engelmann, E. (2002). In vivo interaction of ecdysteroids and juvenile hormone in nymphal and adult cockroaches. J. Insect Sci., 20 :
19. Oehlman, J. and Schulte – Oehlman, V. (2003). Endocrine disruption in invertebrates. Pure Appl. Chem. 75 (11 – 12) : 2207 – 2218.
20.  Subrahmanyan, B. and Rao, P. J. (1986). Azadirachtin effects on Schistocerca gregaria Forskal during ovarian development. Current Science 55 : 534 – 538.
21. Collins, J. V. (1974). Hormonal control of protein sequestration in the fat body of Calpodes ethlius. Can. J. Zool. 52 ; 639 – 642.
22. Sieber, K. P., Rembold, H. (1983). The effect of azadirachtin on the endocrine control of moulting in Locusta migratoria. J. Insect physiol. 29 : 523 – 527.
23. Schluter, V. (1995). Biological effects of Neem and their mode of action. Histopathology, pp. 210 – 222 . In H. Schmutterer (ed.), The neem tree, Azadirachta indica A. Jass. and other meliaceaous plants. Sources of unique natural products for integrated pest management, medicine, industry and other purposes. VCH, Weinheim. Germany, 696 pp.