Document Type : Research Paper

Author

Anbar University - College of Science - Department of Life Sciences

Abstract

The bacterium Kl. pneumoniae was isolated from urine sample of patient with
urinary tract infection and diagnosed according to its cultural and microscopic characters
and biochemical reactions . Sensitivity test of the bacterium against antibiotics was
established and the results appeared that the bacterium was Sensitive to Amoxycillin ,
Gentamycin , Cephaloxin, Neomycin, Rifampicin , Chloramphenicol , Tobramycin ,
Ampicillin and carpenicillin and resistant to clindomycin ,cloxacillin , Trimethprim ,
Cefalotine , Vancomycin , Penicillin G , Lincomycin , Fusidic acid , Metronidazole and
Nalidixic acid .
Results of plasmid DNA content for the bacterium showed the presence of 8-6 kb
plasmid compared with the plasmid pBR 322 isolated from E. coli HBlol . To study the role
of plasmids in coding for antibiotics resistance , curing experiment was accomplished using
SDS .Results showed the loss of resistance of the antibiotic , Nalidixic acid after physical loss
of plasmid as ensured by gel electrophoresis.

Keywords

Main Subjects

 
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