Document Type : Research Paper

Authors

1 Kirkuk University - College of Science

2 Anbar University - College of Education

10.37652/juaps.2009.15576

Abstract

The study involved the isolation and identification of Pseudomonas aeruginosa , thirty four (34)isolates
obtained from (77)samples that were collected from patients attending Kirkuk General Hospital and (2) isolates
obtained from (33) samples that were collected from hospital environment and (24) isolates were collected from Kirkuk
City environment.
The results demonstrated that the pathogenic isolates were resistant to Cefazolin and Carbenicillin by(97.22)%,
where as isolates from Kirkuk City environment were resistant to Cefazolin , Carbenicillin and Cefatoxim by (100% ,
95.5% , 91.6%) respectively.
The results revealed that in Psudomonas aeruginosa the dominant pattern of multiple antibiotic resistance were
(PY,CZ,CTX,CN,TOB) The results obtained showed that one isolates has inhibitory activity against Staphylococcus
aureus . This isolates could possibly be as one of the pyocin producing isolates without using any stimulators.

Keywords

Main Subjects

1- Todor,K.(2004).University of Wisconsin-Madison. Departement of Bacteriology. Pseudomonas aeruginosa.
2-Qarah, S.;  Cunha, B.A.; Dua, P.; Marrie, T.J.; Mylonaskis, E, and zevitz, M.E. (2005). Pseudomonas aeruginosa infection.Medicine from web M.D
3- Marthez,J.L. and Baquero,F.(2002).Interactions among strategies associated with bacterial infection pathogenicity, Epidemicity and antibiotic resistance. Clinical Microbiology Revews. 15 (4): 647-679.
4-Hugo,W.B.and Russel,A.D.(1987).Pharmaceutical Microbiology.4thed Black well scientific publication Oxford,London.
5-الشبیب، اسفار شهاب(1998).علم الاحیاء الدقیقة المعویة .مکتبة دار الثقافة للنشر.
6-Atlas,R.M.(1984).Microbiology.Fundamental and  Application.1steg.Macmillan.Publishing Company.New Yourk.
7- الزیدی، حامد والهام عید عبد الکریم وظمیاء محمود ابراهیم(1987).علم الاحیاء المجهریة العملی. جامعة بغدلد.
8-Baron,E.J.ND Finegold,S.M.(1990).Baily and Scott s Diagnostic Microbiology 8th  ed. The C.V.Mosby Company.U.S.A.
9-Stukus, E.p.(1997).Investigating microbiology.A Laboratory manual for Generalmicrobiology. Company. U.S.A.
10-Macfaddin ,J.F.(200).Biochemical test for identification of medical bacteria. 3rded. Awolters Khuwer Company.Baltimore.
11-Forbes, B.A.; Sahm, D.F.; Weissfeld, A.S, and Trevino, E. A.(2002).Baily and Scott s Diagnostic Microbiology.11thed.Mosby,Inc.St Lousis.
12-Bauer,A.W.;Kirbay,W.A.W.;Sherries,J.S.and Turk,M.(1966).Antibiotic Susceptibility testing by a standarised single disc method. Am,J. Clin. Pathol, 45:493-496.
13-Vandeppitte,J.;Engback,K,;Point,P,and Heuk,C.(1991).Basic Laboratory Procedures In Clinical Bacteriology. World Health Organization, Geneva.
14-Iwalokun,B.A,;Akinsinde,K.A,;Lanlenhin,O,and Onubogu,C.C.(2006).Bacteriocinogenicity and production of pyocins from Pseudomonas species isolated in lagos, Nigeria .African.Journal of Bacteriology.vol.5(11),pp.1072-1077.
15-المحمدی, لهیب رجب حماد (2000) عزل وتشخیص بکتیریا P.aeruginosa مع امکانیة رسم الخارطة الافتراضیة لحرکة هذه البکتیریا بین المصادر المختلفة . رسالة ماجستیر. کلیة العلوم -الجامعة المستنصریة .
16-Orretti, A/F. (2004). Antimicrobial susceptibility survey of  P.aeruginosa strain isolated from clinical sources.J.Microbiol.96(8):1065-106
17-Ruden,H,;Daschner,F,and Schumacher,M.C/(1995).Nosakmiale infectioner in Desutcher and Erfassung and prevention (MDEP-STUDRE)/11 pravlenznosakomider infectioner. Qualitatssicherung Inder Krankenhaushygiene.Schriftenr Bundesministriums Gesundheit.56:53-60.
18- السعدی، زینب شهاب وقاسم، فیصل حسن ،وعبد الکریم فتاح عمر (2005). عزل وتشخیص الجراثیم المسببة لاخکاج السیل التنفسی فی مدینة تکریت وتحدید مقاومتها للمضادات الحیویة مجلة تکریت للعلوم الصرفة.محلد(10)العدد(1).
19-Brown,P.D. and Izundu,A.(2004).Antibiotic resistance in clinical isolates of Pseudomonas Jamaica.Rev Panama Salud Puplica;16(2):125-130.
20-Indudharam,R.;Hag,J.A.and Aiyar,S.(1999).antibiotics in chronic suppurative Otitis Media .Ann. Oto. Rhinol. Laryngol. 108(5) :440-445.
21Wang,K.w,;Chang,W.N,;Shih,T.Y.;Hwang,C.R.;Tsai,N.W.;Chang,c.s.;Chuang,y.c.;Liliang,P.c.;Su,T.m; Rau,C.S.;Tasi,Y.d.;Cheng,B.C.;Hung,P.l.;Chang,C.J.and Lu,C.h.(2004)/Infection of Cerebro Spinal fluid shunt:causative pathogens .Clinical features and outcomes Jpn.j.Infect.Dis.57.44-48.
22-Khalaf,S.H.and Tahir,M.(1986).Bacterial contamination of Municipal drinking water supplies in Fadhilia village near Mosul city. JBSR. Vol. 17. No.2.
23-Levinson,W.(2004).revio of Medical Microbiology an Immunology.McGraw-hill.new yourk.
24jawetz,m.;adelberg,E.A.;Brooks,G.F.;Butel,J.S.and Morse,S.A.(2001).Medical microbiology. Twenty-Second edition McGraw – Hill Company , New yourk.
25-الشویخ ،رنا مجتهد عبد الله (2006).انتاج وتوصیف protease من بکتیریا P.aeruginosa المعزولة من حالات مرضیة وعلاقته ببعض مضادات الحیاة.رسالة ماجستیر،کلیة العلوم،الجامعة المستنصریة.
26-saeed, R.S. (1993). Proteus and other microorganisms causing U.t.i.in 26- Children M.Sc. University of bagdhad.
27- Michea-Hamzehpour,M.;Kahar,A.and Pechere, J.C. (1994).in vivo selection of resistance to  qunnolones , b-lactams and amikcin in nasocomial gram-negative bacilli.infection22,(suppl.2):105-110.
28- Lyobe,S.;Tsunoda,m.and Mitsuhashi, S.(1994). cloning and expression in Enterobacteriacea of the Extended spectrum b-lactamase gene from P.aeruginosa plasmid. Microbial. 121:175-180.
29- moyneimne,H.;Robert,J.;Jarlier,V. and Cambau, E.(1999).type IIto poison erase mutation in ciprofloxacin resistant strain of P.aeruginosa .Antimicrob.Agents.Chemo,43(1):62-66.
30- harder,K.;Nikadio,H.and Mastuhashi,m. (1981). Mutant of E.coli that are rresistant to certain B-lactam compound lackthe ompF proin . Antimicrob. Agents.Chemo.20:549-552.
31- Wilcox, M.h.; Winstanley, T.G. and spencer,r.c. (1994). outer membranes protein profiles of Xanthomonas matophilia  isolates displaying temperature. Dependint susceptibility to Gentamicin. Antimicrob.Agents.Chemo.33:663-666.
32- Jacoby, G. A. and Archer, G.l. (1991). New mechanisms of bacterial resistance to antimicrobial agents.New England J .of medical .324-360.
33-Duport,C.;Baysse,C.and Riand,Y.m. (1995). Molecolar characterization of the pyocin S3 ,a Novel   S  .type pyocin from P.aeruginosa .B. American Socity for Biochemistry and Molecular Biology Vol.270 No.15.Issue of April 14,pp.8920-8927.