Document Type : Research Paper

Author

College of Medicine, Anbar University

Abstract

Fifty urine samples were collected from patients with urinary tract infections and a 50 stool specimens from diarrhoeal patients . These samples were investigated bacteriologically. The invitro adherence test of Enteropathogenic and Uropathogenic E. coli to healthy human buccal epithelial cells was carried out. The results show no significant differences in the mean adhesion of these bacteria (two sources) on the epithelial cells as compard with the control . but there is a little variation among EPEC isolates and among UPEC isolates too as compared with the control.

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1- Reed, W.P. and Williams, R.C. (1978). Bacterial adherence: first step in pathogenesis of certain infections. J. Chron. Dis. 31:67-72.
2- Maskell, R. (1988). Pathogenesis, consequences & natural history of UTI: Adhesion to epithelium. In: Urinary tract infection in clinical and laboratory practice. Ch.1, p: 1-25. Edward Arnold.
3- Fowler, J.E., and Stammey, T.A. (1978). Studies of introital colonization in UTI adhesive properties of E.coli with urinary pathogenicity, mirabilis, lack of correlation with urinary pathogenicity. J. Urology Vol. 120 page 315-318.
4- Jackson, G.G., and Riff, L.J. (1971). Pseudomanas bacteria: pharmacologic and other bases for failure of treatment with gentamicin. J. Infect. Dis. 124 (suppl.): s185-s191.
5- Peter Pieroni, Elizabeth A., Worbec, Paranchych, W., and Glen, D. Armstrong (1988). Identification of a human erythrocyte receptor for colonization factor antigen I pili expressed by H10407 Enterotoxigenic E. coli. J. Infect. and Immun. Vol. 56. No. 5 page 1334-1339.
6- Goldhar, J., Perry, R., Golecki, J.R., Hoschutzky, H., Jann, B. & Lann, K. (1987). Non fimbrial, mannose resistant adhesins from uropathogenic E. coli O83: K1H4 & O14: K? H11. Inf. and Immun., 55; 1837-1842.
7- Rosenstein, .J., Mizuoch, T., Hounsell, E.F., Stoll, M.S., Childs, R.A., and Feizi, T. (1984). Probes in E. coli from patients with urinary tract infection. Lancet 10:1327-1330.
8- Salit, I.E. and Gotschlich, E.C. (1977a). Type-1 E. coli pili: characterization of binding to monkey kidney cells. J. Exp. Med. 146:1182-1194.
9- Vaisanen, V., Lountmaa, K., and Korhonen. (1982). Effect of sublethal concentrations of antimicrobial agents on the haemagglutination, adhesion and ultrastructure of pyelonephritic E. coli strains. Antimicrob. Agents. Chemother. 22:120-127.
10- Sugarman ,B, and S.T. Danta, (1979) Adherence of Enterobacteriacea to human buccal cells. J.med. microbial. 12:373-78.
11- Jawetz, E., Melnick, J.L., Adelberg, E.A (1978). Review of medical microbiology. 13th ed.
12- Lewis, D.A. (1989). Bacteriology of urine. In: Medical Microbiology a practical approach. Hawkey, P.M., Lewis, D.A. (eds).
13- Stokes, E.  and Ridgway, G.L. (1987). Clinical bacteriology. 5th edition. Cited by Al-Ubaidy, 1990.
14- Cappuccino, J.G. and Sherman, N. (1987). Microbiology a laboratory manual.
15- Parker, M.T.(1983). Pseudomonas. In: Topley and Wilson’s principles of bacteriology, virology, and immunology. Vol. II Ch. 31 P: 246.
16- Leffler, H. & Eden, C., (1981). Glycolipid receptors for uropathogenic E.coli on human erythrocytes and uroepithelial cells. Inf. & imm. 34(1-3); 920-929.
17- Lomberg, H., Cedergren, B., Loffler, H., Nilsson, B., Carlstrom, AS., and Eden, C. (1986).  Influence of blood group on the availability of receptors for attachment of uropathogenic E. coli. Inf. Immun. Mar. page 919-926.
18- Eden, C.S., Eriksson, B., and Hanson, L.A. (1977). Adhesion of E. coli to human uropithelial cells. Infect. Immun. 18: 767-774.
19- Hagberg, L., Jodal, U., Korhonen, T.K., Janson, G.L., Lindberg U., and Eden C.S. (1981). Adhesion, heamagglutination, and virulence of E. coli causing urinary tract infections. Infect. Immun. 31: 564-570.
20- Iwahi, T., Abe, Y., Nakao, M., Imada, A. and Tsuchiya, K. (1983). Role of type-1 fimbriae in the pathogenesis of the ascending urinary tract infection by E. coli in mice. Infect. Immun. 39: 1307-1315.
21- Sato, H., and Okinaga, (1987). Role of pili in pathogenesis of Ps. aeruginosa burn infection. Microbiol. Immunol. 32: 131-139.
22- Kallenius, G., Mollby, R. (1979). Adhesion of E. coli to human periurethral cells correlated to mannose resistant agglutination of human erythrocytes. FEMS Microbiol letter. 5: 295-299.
23- Kallenius, G. & Winberg, J. (1978). Bacterial adherence to periurethral epithelial cells in girls prone to urinary tract infections. Lancet, 2; 540-543.
24- Kern, W.H. (1970). Epithelial cells in urine sediments. Am. J. clin. Pathol. 56: 67-72.
25- Al-Ubaidy, N.M.A. (1990). Role of pili and other natural characters of Escherichia coli causing urinary tract infection. M.Sc. thesis Coll.of Med, Univ. of Baghdad.