Document Type : Research Paper

Author

Department of Biology - College of Science - University of Anbar

Abstract

The bacterium Pseudomonas aeruginosa was isolated from patients of
burns in Al-Ramadi hospital and diagnosed according to its cultural , microscopic
and biochemical properties. sensitivity test of the bacterium against antibiotics was
established and the results appeared it was sensitive to the antibiotics Carbenicillin ,
Chloramphenicol , Rifampicin and Trimethoprim and resistant to the antibitcs
Amoxicillin , Ampicillin Clindomycin Cloxacillin , Nalidixic acid , Cephaloxin ,
Tetracyclin and Tobromycin . Results of mutagenesis showed resistance of the
bacterium to the lethal effect of nitrous acid and its sensitivity to the mutagenic effect
of the acid relative to the antibiotics Rifampicin and Chloramphenicol but not to the
antibiotics Carbenicillin and Trimethoprim .

Keywords

Main Subjects

]1[Auerbach , c. (1962) . Mutation ,an introdution to research on mutagenesis . Oliver and Boyed , Edinburgh .
]2[Shortle , D . D . , Maio , D . and Nathans , D. (1981) . Directed Mutagenesis . Ann.Rev.Biochem . 15:265-294.
]3[Singer , B.and Kusmierek , J . T . (1982). chemical mutagenesis . Ann.Rev.Biochem. 51: 655 – 693 .
 [4]Al- Habib , H.M. and Al-Talib , A.B. (1985). pseudomonas aeruginosa  isolated from four types of infections and its sensitivity to  antibiotics . journal of faculty of Medicin (Baghdad) 27: 35-43
[5]Al-Bakri,G.H.and Umran , M.A. (1994) Mutagenesis of a novel halotolerant bacteria (Micrococcus SP.) using ultraviolet light and N-Methlyl –N-nitro-N-Nitroso- guanidine Iraqi Journal of Microbiology , vol. 6(2): 55-64 .
]6[Bowring , S.N. and Morris , J . C . (1985) – Mutagenesis of Clostridium acetobutylicum . J . APPI . Bacteriol . 58:577-584.
]7[Kerszman , G . (1975) . Induction of mutation to Streptomycin resistance in Micrococcus radiodurans . Mutation Res . 28: 9-14 .
]8[Frifelder , D . (1987) . Molecular biology – 2nd . Yones and Barttett Boston .