Document Type : Research Paper

Authors

1 College of Science, University of Anbar

2 College of Education for Girls, University of Anbar

Abstract

Leukemia is one of the most common cancer prevalent worldwide in both gender, and all races regardless of the level of living. It affects blood-forming tissues especially the bone marrow. Due to the importance of this disease and its risks, the study was carried out to evaluate the oxidative stress status and detect the effect of leukemia on some hematological parameters. Estimate gamma glutamyle transferase (GGT). A study was conducted between August 2016 till February 2017 in the center for Hematology/ Yarmouk Hospital. The blood samples were collected from 70 Iraqi patients with leukemia and 37 normal persons as control group, their ages ranged from (6-68 years). The statistical analysis of malondialdehyde (MDA) showed significant increase (P≤ 0.05) in patients with leukemia in compared to healthy. While there was significant decrease in Vitamin C and E concentration in patients (7.10 ± 0.49; 8.75 ± 0.65 µmol/L respectively) in compared with healthy group (18.83 ± 0.67; 25.83 ± 0.88 µmol/L respectively). The results of gamma glutamyle transferase (GGT) showed significant increase (P≤ 0.05) in patients with leukemia compared to healthy group. The results of hematological parameters showed significant decrease (P≤ 0.05) in each of packed cell volume (PCV) in patients with leukemia compared to healthy group, and hemoglobin concentration in patients compared to healthy group, while there was a significant increase (P≤ 0.05) in total leukocytes count in patients with leukemia when compared to healthy group. The results of the study showed a positive correlation between GGT and WBC.

Keywords

Main Subjects

1-      Shnjar AM. Detection of N-Ras & H-Ras Mutations Associated with acute and chronic leukemia . a thesis submitted for the degree of the master in Genetic Engineering and Biotechnology, Iraq Journal Hematolgy. 2013; 22(7): 17-21.
2-      Liao R, Xu Y, Chen M, Chen X, Zhan X and Sun J. Molecular mechanism of microRNA involvement in genesis of myelodysplastic syndrome and its transformation to acute myeloid leukemia. Hematology. 2013;18(4):191–7.
3-      Wiemels JL, Kang M, Chang JS. Backtracking RAS mutations in high hyperdiploid childhood acute lymphoblastic leukemia. Blood Cells Mol Dis. 2010; 45(8):122-143.
4-      Huang JC, Basu SK, Zhao X, Chien S, Fang M, Oehler VG and Becker PS. Mesenchymal stromal cells derived from acute myeloid leukemia bone marrow exhibit aberrant cytogenetics and cytokine elaboration. Blood Cancer Journal. 2015; 5(4): 302-306.
5-      Kryston TB, Georgiev AB, Pissis P and Georgakilas AG. Role of oxidative stress and DNA damage in human carcinogenesis. Mutation research. 2011; 711(9): 193-201.
6-      Mendivil-Perez M, Velez-Pardo C and Jimenez-Del-Rio M. TPEN induces apoptosis independently of zinc chelator activity in a model of acute lymphoblastic leukemia and ex vivo acute leukemia cells through oxidative stress and mitochondria caspase-3- and AIF-dependent pathways. Oxidative Medicine and Cellular Longevity. 2012; 32(4): 33-49.
7-      Pereira RJ and Cardoso MDG. Metabólitos secundários vegetais e benefícios antioxidantes Vegetable secondary metabolites and antioxidants benefits. Journal of Biotechnology and Biodiversity. 2012; 3(11): 146-152.
8-      Voulgaridou GP, Anestopoulos I, Franco R, Panayiotidis MI and Pappa A. DNA damage induced by endogenous aldehydes: current state of knowledge. Mutation Research. 2011; 711(1–2): 13-27.
9-      Ahmad R, Tripathi AK, Tripathi P, Singh S, Singh R and Singh RK. Malondialdehyde and protein carbonyl as biomarkers for oxidative stress and disease progression in patients with chronic myeloid leukemia. Heamatology, (Athens, Greece). 2008; 22(4): 525-528.
10-  Suhail N, Bilal N, Khan HY, Hasan S, Sharma S, Khan F, Mansoor, T and Banu N. Effect of vitamins C and E on antioxidant status of breast-cancer patients undergoing chemotherapy. Journal of Clinical Pharmacy and Therapeutics. 2012; 37(9): 22–26.
11-  Schlueter AK and Johnston CS. Vitamin C: Overview and Update. Journal of Evidence-Based Complementary & Alternative Medicine. 2011; 16(1): 49-57.
12-  Stanley T, David T and Howerds S. Selected method for the determination of ascorbic acid in Animal cells, tissues and fluids. Method in Enzymology. Vitamins and coenzymes part D, 1979; pp.62.
13-  Varly H, Gowenlock AH and Bell M. Practical clinical biochemistry. Harold Varly, Great Britain. 1976; 15(2): 43-41.
14-  Fong KL, Cay PB and Poyer JL. Evidence that peroxidation of lysosomal membranes is initiated by hydroxyl free radicals produced during flavin enzyme activity. Journal Bio Chem. 1973; 248(7): 7792-7797.
15-  Szasz G and Bergmeyer HU. Method of Enzymatic analysis. Weinheim Veriage Klin Chim Biochem. 1974; 12:228.
16-  Markarem A. Clinical Chemistry – Principles and Techniques. 2nd. ed., Herny, D.C.' Cannon, J.W. and Winkelmen editor. Harper and Raw. New York, Hargeston. 1974; pp.1128-1135.
17-  Hillman RS and Ault KA. Hematology in Clinical Practice. McGraw-Hill. 2002; 3(2): 46-47.
18-  Lewis SM, Bain BJ and Bates I. Haematology. Livingston Churchill. 2001; 19(5): 589-601.
19-  Manzoor SM, Sattar A and Hashim R. Serum inhibin b as a diagnostic marker of male infertility. Journal Ayub Med Coll Abbottabad. 2012; 24(8): 6-113.
20-  Demir C, Demir H, Ramazan R, Atmaca M and Tasdemir E. Erythrocyte catalase and carbonic anhydrase activities in lung cancer. Asian Pacific Journal of Cancer Prevention. 2010; 11(5): 1377-1382.
21-  Xavier OD, Giraltb M, Romeub DM, Lejeunec M, Noguesb MR, Vanessa SM, Luacesa MR, Sansa T and Fonta L. Oxidative stress in patients with early stage chronic lymphocytic leukemia, assessment and correlation with prognostic factors. Hematology Journal. 2012; 4(5): 77-88.
22-   Sisein EA. Biochemistry of Free Radicals and Antioxidants. Scholars Academic Journal of Biosciences. 2014; 2(2): 110-118.
23-   Wu D and Yotnda P. Production and Detection of Reactive Oxygen Species (ROS) in Cancers. Journal of Visualized Experiments. 2011; 8 (57): 2-5.
24-  Al-ubuda NM, Al-naama LM and Al-hashimi AH. Profile study of some oxidant and antioxidant levels in leukemic patients. The Medical Journal of Basrah University. 2012; 30(2): 115-121.
25-  Pujari KN, Jadkar SP, Mashal SN, Belwalkar GJ, Patil CG and Tuljapurkar VB. Variations in vitamin C levels in leukemia. Hematology. 2012; 23(2): 307-311.
26-  Saadaoui M, Aissaoui L, Salaun V, Manai M and Allouche S. Mitochondrial DNA Alterations and Oxidative Stress in Acute Leukemia. The Open Leukemia Journal. 2013; 5(10): 1-6.
27-  Darias MJ, Mazurais D and Koumoundouros G. Imbalanced dietary ascorbic acid alter molecular pathways involved in skeletogenesis of developing European sea bass (Dicentrarchuslabrax). Company Biochemical Physiolgy and Molecular Integr Physiology. 2011; 159(8): 46-55.
28-   Mehde AA, Mehdi WA, Zainulabdeen JA and Abdulbari AS. Correlation of inhibin and several antioxidants in children with acute lymphoblastic leukemia. Asian Pacific Journal of Cancer Prevention. 2014; 15(12): 4843–4846.
29-  El-Sabagh ME, Ramadan KS, El-slam IMA and Ibrahim AM. (2011). Antioxidants Status in Acute Lymphoblastic Leukemia Patients. American Journal of Medicine and Medical Sciences. 2011; 1(1): 1-6.
30-   Khalid N, Mustafa EE, Sheikha A, A-J Thanoon I and Hussein FN. (2008). Effect of vitamin E on chemotherapy induced oxidative stress and immunoglobulin levels in patients with acute myeloid leukemia. Tikrit Medical Journal, 14(1): 146-151.
31-  Zainulabdeen JA. Correlation between malondialdehyde and metanephrine in patients with acute lymphoblastic leukemia Abstract. Intrroduction. Material and methods. Hematolgy. 2014; 45(8): 33-79.
32-  Mallouh SS, Alawadi NB and Hasson AF. Serum Vascular Endothelial Growth Factor Levels in Iraqi Patients with Newly Diagnosed Acute Leukemia. Blood. 2015; 12(1), 21(7), pp.11-32.
33-  Alter BP. Fanconi anemia and the development of leukemia. Best Practice and Research. Journal of Clinical Haematology. 2014; 23(2): 812-833.
34-   Veena S, Munish K, Kiran D, Rakesh D and Ragini S. Erythrocytic Pyruvate Kinase and Malondialdehyde Levels in Acute Leukaemia Patients. Journal CDR. 2012; 6(3): 361-363.
35-  Yousif A and Info A. Estimation of Malondialdehyde , Total Antioxidant Capacity and Some Biochemical Parameters in CSF and Sera of Patients with Acute Lymphoblastic Leukemia. Australian Journal of Basic and Applied Sciences. 2014; 8(9): 329-333.
36-  Wang L, Lawrence MS and Wan Y. (2011). SF3B1 and other novel cancer genes in chronic lymphocytic leukemia. N Engl Journal Med. 2011; 365(26): 2497-506.
37-  Liu Y, Li P, Lu J, Xiong W, Oger J, Tetzlaff W and Cynader, M. Bilirubin possess powerful immunomodulatory activity and supressess experimental autoimmune encephalomyelitis. Journal Immunol. 2008; 181(3): 1887 -1897.
38-   Kream RM. Nitric oxide regulation of mitochondrial processes: commonality in medical disorders. Annals of Transplantation. 2015; 0(8): 402-407.
39-  Abdul Sattar, Khanam A, Ibad S and Iftikhar A. Drug Induced Hepatotoxicity After Introduction Phase of Chemotherapy in Acute Lymphoblastic Leukemia Patients. European Journal of Pharmaceutical and Medical Research. 2016; 3(8): 111-115.
40-  Segal I, Rassekh SR, Bond MC, Senger C and Schreiber RA. Abnormal liver transaminases and conjugated hyperbilirubinemiaat presentation of acute lymphoblastic leukemia. Blood Cancer. 2010; 55(99): 434-439.
41-   Khaled SAA. ABO and Rhesus Blood Groups. Possible Entities in the World Health Organization Classification Acutenon – Lymphoblastic Leukemia. Journal of Research in Medical Sciences.
42-   2013; 11(4): 239-258.
43-  AL-leban W, Ali A and Shweliya HM. (2015). Epidemiological Study of Chronic Myeloid Leukemia Patients. IOSR Journal of Pharmacy and Biological Sciences. 2015; 10(2): 2319-7676.
44-  Alaa FA, Zedan JZ, Omar SS. Acute myeloid leukemia: clinical features and follow-up of 115 Iraqi patients admitted to Baghdad Teaching Hospital. Journal Tikrit Medicine. 2009; 15(1): 1-8.