Molecular study to some Virulance gene of Staphylococcus aureus and Pseudomonas aeruginosa bacteria isolated from women suffered from bacterial vaginitis

Farhan,, Wrood Mutleb; Abd AL-latef Yaseen, Hanaa

doi:10.37652/juaps.2022.171457

Document Type : Research Paper

Authors

Department of Biology, College of Education for woman, University of Anbar

https://doi.org/10.37652/juaps.2022.171457

Abstract

The study included 250 vaginal swabs were collected from women patients clinical symptoms of bacterial vaginitis and they have come to the women's and children's hospital in Ramadi and ages (17- 45 years) nearly, to Investigation of the bacterial species positive and negative gram stain were Infected of the vagina, and also To determination the appearance and molecularly of virulence factors. The vaginal swabs were cultured, 86 samples were found to give positive growth of 34.4%, but (164 sample) 65.6% were negative. The growing isolates were subjected to bacteriological and biochemical tests. The isolates of positive gram stain 48 about 55.81% were having character of Staphylococcus epidermidis 18 about 20.93% and Staphylococcus aureus (9 (10.46%), Streptococcus pneumoniae 7 nearly 8.13%, Staphylococcus saprophyticus 6 about 7.97%, Streptococcus pyogenes 4, (4.65%) and Bacillus subtilis only 3, (3.48%) and Streptococcus viridans was only 1 isolate about 1.16% . The isolates of negative gram stain were recorded 38 percentage 44.18% . The results of the tests a bacteriological and biochemical were recorded Escherichia coli 15 isolates percent 17.44% and 14 of Klebsiella pneumoniae 16.27% , Pseudomonas aeruginosa was 6 percent 7.97%, Gardnerella vaginalis 2 about 2.32% , and Proteus mirabilis was one isolate only percent 1.16% . The polymerase chain reaction (PCR) technique were proved, the exoA and exoS genes were encodes toxin production. algD was encoded the production of the capsule which were found in Pseudomonas aeruginosa percent 100% for the three genes. In Staphylococcus aureus found that (hla) gen that encodes production of alpha-hemolycin 66.66% and finbA which encodes the production of (adhesion protein) 100%, as well as investigate some of the virulent factors of the vaginal bacteria, which included their ability to produce the enzyme hemolysis, urease and protease.

Keywords

Main Subjects

Biology

References

1- Deborah, B. N. and George, M. (2002). Bacterial Vaginosis in pregnancy: current finding and future direction . Epidmio1 Rev. Vol.24 P:102-108.

2-علی، منى جلال (2010). دراسة عوامل الضراوة للجراثیم المسببة لالتهاب المهبل البکتیری لدى النساء. رسالة ماجستیر، قسم تقنیات الموارد المائیة، المعهد التقنی الحویجة.

3-Mark, H.; yadin, M.D.; Deborah, M. and Money, M.D. (2008) Screening and Management of Bacterial Vaginosis in Pregnancy. SOGC Clinical Practice guideline P: 702-708.

4- William, W.B. (1997) .Obstetrics and Gynecology 4^th ed.,Edited by Williams and wilikins, middle East Ediation, Egypt .

5-Carolin, M.; Hara,O.; Frances,W. and Michael,J.M.(2000) . Classification, identification and Clinical significance of Proteus, Provedencia, and Morganella. Vol.13 No.(4) P: 534-546 .

6- Iwona, B.; Julin, F. and Jaunne, N.(2012) Pseudomonas aeruginosa pili and Flagella Mediate Distinact Binding and Signaling Events at the Apical and Basolateral surface of Air way Epithelium . International Journal of Antimicrobial Agents ;Vol. 42 P: 211-219.

7-Dmitri V. ; Robert F.; Phillips G. and Linda S. (2001). Functhonal analysis of genes for biosynthesis of pyocyanin and phenazine 1-carboxamide for Pseudomonas aeruginosa PAO1. Journal of Infection Disease; Vol.193 P:645-54.

8- Matar, G.; Ramlaw, N.; Hijazi, I. and Khneisser, A. (2002). Transcription levels of Pseudomonas aeruginosa exotoxin A Gene and severity of Symptoms in patients with otitis external curr. Micro. J.; Vol. 16 (2), PP. 167-172.

9- Jiang, X.; Zhang, Z. L.; Zhou, D.; Ruan, F. and Lu,Y.(2014). Detection,F. extended-spectrum B-Lactamase in clinical isolates of Pseudomonas aeruginosa. Ant-imicrob agent and chemother, Vol.50.p.2990-2995.

10- Moravej,Z.; Tabatabaei, M.; Shirzad, A. and Khoshbakht,, R.(2015) .Characterization of hemolysins of Staphylococcus strains isolated from human and bovine, Southern lran. Iranian Journal of veterinary Research . Vol.15 No.(4) P:326-330..

11- جواد، مصطفى رعد، یوسف، میثم غالی(2012). التحری عن عوامل الضراوة والمقاومة للمضادات الحیویة فی البکتریا المعزولة من حالات سریریة مختلفة فی مدینة الدیوانیة. قسم علوم الحیاة، کلیة العلوم، جامعة القادسیة. ص:1-22.

12-Benson, H.J. (2002). Microbiological applications : laboratory manual in general microbiology. (8^th ed.), complete version. McGraw-Hill. U.S.A

13 ابو ریشة، رسمیة عبد، سلوم، دنیا فرید وخلیل، لمیس (2007(. دراسة تأثیر الفا هیمولایسین المنتج من Staphylococcus aureus على عملیة البلعمة خارج الجسم الحی . المجلة العراقیة للعلوم، قسم علوم الحیاة، کلیة

14- MacFaddin, J.E.(2000). Biochemical test for identification of medical bacteria. “3^rd ed.’’. Lippn CottWilliams and wilkins. Philadelphia. U.S.A.

15- Harding, E.N.; Cleary, J.M.; Cabanas, P. K.; Rosen, I.G.and Kang, K. S.(1987) . Genetic and physical analyses of acuter of genes essential for xanthan gum biosynthesis in Xanthomonas campestris. J Bacteriol .169(6) : 2854 -2861.

16- Sedighe,C.R.; Behzad, K.N.;Nezhad,H. and Mohammed, N.E.(2013). Detection of algD, oprL and exoA Genes by new specific primers as an efficient, rapid and Accurate proceder for direct diagnosis Pseudomonas aeruginosa strains inclinical samples . Jundishapur microbial . 7(10): e13583 .

17-Lanotte, P. ;Watt, S. ; Mereghetti,L. Goudeau, A. (2004) Genetic features of P. aeruginosa isolated from cystic fibrosis Patient compared with those of isolates from other orings . . Journal of medical Microbiology . 53, 73-81 .

18-Mary,C. Booth, M. Michelle, C. and Michael, S.(2001) Clonal Association among Staph. aureus Isolated from Various sites of infection, Infection and Immunity P, 345-352 .

19-الحدیثی، درید صیهود ( 2010) . دراسة فعالة المضادة للبکتریا فی بعض النباتات الطبیة ضد البکتریا المسببة لالتهاب المجاری البولیة . کلیة العلوم، قسم علوم الحیاة . الجامعة الحرة فی هولندا.

20- Spiegel, C.A. and Amsel, R. and Holmes, K.K. (1983). Diagnosis of bacterial vaginosis by direct gram stain of vaginal fluid. Us national library of Medical. Vol.18No.(1) P: 170-7.

21- العانی، احلام عجاج (2006) . دراسة تأثیر مستخلصات نبات الاشنان Seidizia rasmorinus على نمو بعض الجراثیم المرضیة المسببة لالتهابات المهبلیة . رسالة ماجستیر، کلیة العلوم، جامعة بغداد .

22- الیاسری، اسراء عتاب، الشامی . زینه محمد (2005). دراسة بکتریولوجیة لعزل وتشخیص بکتریا المکورات العنقودیة الذهبیة Staph. aureus المتعددة المقاومة للمضادات الحیویة والمعزولة من عینات سریریة مختلفة فی محافظة النجف. کلیة الطب، جامعة الکوفة .

23- Lindsey, S.; Ewa, G.; Jan, P. and simon, J.F. (2004). The role and regulation of the extracellular proteases of staphylococcus aureus. Microbiology. Vol.150, P: 217- 228.

24- Kazuyuki, M. and Hiroshige, T. (1977). Production of protease and Elastase by Pseudomonas aeruginosa strains Isolated from patients. American society for Microbiology. P: 679-695.

25- Alina –Maria, H.; Mariana, C.C.; Ani, I. C.; Coralina, B. and Veronica,L. (2013). Virulance markers in Pseudomonas aeruginosa isolated from hospital- aqaired infections occurred in patients with underlying cardiovascular disease, Romanian Biotechnological Letters. University of Bucharest. Vol. 18. P: 8843-8854.

26- Katarzyna, W. and Piotr, S. (2009) .Genetic features of clinical Pseudomonas aeruginosa strains. Vol. 58 No. (3).P: 255-260.

27- Sedighe, R.T. ; Behzad, K. and Ensanollah, G.(2013). Detection of alg D, opr L, and exo A Genes by new specific primers as an Efficient, Rapid and Accurate procedure for Direct Diagnosis of Pseudomonas aeruginosa strains in clinical sample, Jundishapur,Journal of Microbiology.

28- Mohammed, E.A. ;Ismail, H. A.and Abeer, A., M. (2014).Identification pseudomonas aeruginosa species that isolated from patients and environment .Baghdad science Journal. Vol. 11No.(2) P:1028-1034.

29- الناشی، علی عبد الرحیم، المنصوری، ریام وسام حسن (2016) . التحری عن الطراز المظهری والجزیئی لعوامل الضراوة لبکتریا Staphylococcus spp. المحللة للدم والمعزولة من التهاب المسالک البولیة فی الدیوانیة . رسالة ماجستیر، کلیة التربیة، جامعة القادسیة .

30- Silva, P. V. ; Cru Z, R.S; Keim, L.S.; Depula, G.R.;Carvalho, M. C. D., Rosa, J.M.(2013).The antimicrobial susceptibility biofilim formation and genotypic profiles of staph. Haemolyticus from blood stream infection. Men inst. Oswaido.Cruz. Rio. De. Janeiro, 108 (6): 812.816.

31- Baba- Moussa, L.; Anani. L.; Scheftel, J.; M couturier, M.; Haikou, N.; Hounsou, F.; Monteil, H.; Sanni, A. and prevoste G. (2008). Virulnace factors produced by strains of Staphylococcus aureus isolated from urinary tract infection. J. Hosp. infect,Vol (968) P: 32-38.

Journal of University of Anbar for Pure Science

Molecular study to some Virulance gene of Staphylococcus aureus and Pseudomonas aeruginosa bacteria isolated from women suffered from bacterial vaginitis

References

References

Volume 12, Issue 1
April 2018
Page 18-30

Molecular study to some Virulance gene of Staphylococcus aureus and Pseudomonas aeruginosa bacteria isolated from women suffered from bacterial vaginitis

References

References

Volume 12, Issue 1April 2018Page 18-30

Volume 12, Issue 1
April 2018
Page 18-30