Document Type : Research Paper


1 alanbar education diuractorate

2 Anbar Education Directorate, Ministry of Education, Anbar, Iraq

3 1 College of Medicine, University of Anbar, Ramadi, Iraq.

4 3Education for Pure Sciences College , University of Anbar.

5 4National Diabetes Center/ Mustansiriyah University, Baghdad, Iraq.

6 Facully of Medicine ,University of Jordan, Amman, Jordan.


Background: Menopause is the stage of a woman's life when her menstrual cycles cease for good. In the early stages of menopause, cardiovascular risk significantly rises, primarily as a result of the loss of estrogen's protective effects. Objective: The objective of the current study was to measure the prolactin level in menopausal diabetic Iraqi women as well as the correlation between prolactin levels and other biochemical markers. Methods: This study was carried out on fifty patients women with diabetes type 2 during menopause aged (40–48 years) who visited the National Diabetes Center/AL-Mustansiriya University, Baghdad-Iraq. On the other hand, Forty healthy women without diabetes aged (40–48 years) were recruited as control. The study included measurements of the biochemical parameters such as, glycated hemoglobin HbA1c by using (Bio-Rad device, France), Prolactin (PRL) estimated by using (minividas device, Biomerieux-France).Fasting blood sugar (FBS), total cholesterol (TC), triglycerides TG, and high-density lipoprotein HDL were evaluated enzymatically by an auto-analyzer (Kenza 240 TX analyzer) (Biolabo, France).Results: The clinical biochemical markers showed that the serum levels of prolactin, fasting blood sugar (FBS), body mass index (BMI), HBA1c, TC, TG, low-density lipoproteins LDL and very low-density lipoproteins (VLDL) were increased significantly, while the decreased level of serum (HDL) in diabetic women of menopause as compared with healthy women (P=0.0001). Conclusion: As a result of the considerable changes in prolactin levels and altered serum lipid profiles associated with menopause, cardiovascular disease development is at increased risk on its own.


Main Subjects

[1]   M. Wang et al.,.(2018).“Age at natural menopause and risk of diabetes in adult women: Findings from the China Kadoorie Biobank study in the Zhejiang area,” J. Diabetes Investig., vol. 9, no. 4, pp. 762–768, , doi: 10.1111/jdi.12775.
[2]   Z. Xing, R. S. Kirby, and A. C. Alman,.( 2022).“Association of age at menopause with type 2 diabetes mellitus in postmenopausal women in the United States: National Health and Nutrition Examination Survey 2011–2018,” Prz. Menopauzalny, vol. 21, no. 4, pp. 229–235, doi: 10.5114/pm.2022.123514.
[3]   G. A. Georgiopoulos et al.,.(2009).“Prolactin and preclinical atherosclerosis in menopausal women with cardiovascular risk factors,” Hypertension, vol. 54, no. 1, pp. 98–105,doi: 10.1161/HYPERTENSIONAHA.109.132100.
[4]   A. Monterrosa-Castro et al.,.( 2013).“Type II diabetes mellitus and menopause: A multinational study,” Climacteric, vol. 16, no. 6, pp. 663–672, doi: 10.3109/13697137.2013.798272.
[5]   A. Nanri, T. Mizoue, M. Noda, A. Goto, N. Sawada, and S. Tsugane,.(2019).“Menstrual and reproductive factors and type 2 diabetes risk: The Japan Public Health Center-based Prospective Study,” J. Diabetes Investig., vol. 10, no. 1, pp. 147–153, doi: 10.1111/jdi.12853.
[6]   M. Wang et al.,.(2022).“Menopausal status, age at natural menopause and risk of diabetes in China: a 10-year prospective study of 300,000 women,” Nutr. Metab., vol. 19, no. 1, pp. 1–12, doi: 10.1186/s12986-022-00643-x.
[7]   T. Lathia and S. Kalra,.(2020).“The Interrelationship of Menopause and Type 2 Diabetes Mellitus,” Diabetes Res. – Open J., vol. 6, no. 1, pp. 20–26, doi: 10.17140/droj-6-146.
[8]   G. K. and S. N. Sheoran A , Agarwal N , Mahto S K, Gupta P K,.( 2023).“Study of Association of Serum Prolactin Levels with Insulin Resistance in Type 2 Diabetes Mellitus Patients,” Indian J. Endocrinol. Metab., pp. 1–6, doi: 10.4103/ijem.ijem.
[9]   A. Amirzadegan, E. Salimi, and A. Hosseinsabet,.(2019).“Is prolactin serum level and coronary artery atherosclerosis correlated in postmenopausal women? A cross-sectional study,” Turk Kardiyol. Dern. Ars., vol. 47, no. 3, pp. 177–184, doi: 10.5543/tkda.2018.45213.
[10] Y. Zhang and H. Liu,.(2022).“Cross-sectional association between prolactin levels and non-alcoholic fatty liver disease in patients with type 2 diabetes mellitus: a retrospective analysis of patients from a single hospital in China,” BMJ Open, vol. 12, no. 10, pp. 1–8, doi: 10.1136/bmjopen-2022-062252.
[11] L. Faria de Castro, Á. Alves dos Santos, L. Augusto Casulari, L. Ansaneli Naves, and A. Amorim Amato,.( 2020).“Association between variations of physiological prolactin serum levels and the risk of type 2 diabetes: A systematic review and meta-analysis,” Diabetes Res. Clin. Pract., vol. 166, doi: 10.1016/j.diabres.2020.108247.
[12] M. Daimon et al.,.(2017).“Association between serum prolactin levels and insulin resistance in non-diabetic men,” PLoS One, vol. 12, no. 4, pp. 5–7, doi: 10.1371/journal.pone.0175204.
[13] J. Chen, G. Liu, Q. Li, and W. Deng,. (2022). “Prolactin is associated with bone mineral density in subjects with type 2 diabetes mellitus,” Front. Endocrinol. (Lausanne)., vol. 13, no. October, pp. 1–10, doi: 10.3389 /fendo. 2022. 964808.
[14] R. M. Ibrahim et al.,.(2014).“A randomised controlled trial on hypolipidemic effects of Nigella Sativa seeds powder in menopausal women,” J. Transl. Med., vol. 12, no. 1, pp. 1–7, doi: 10.1186/1479-5876-12-82.
[15] V. Inaraja, I. Thuissard, C. Andreu-Vazquez, and E. Jodar,.(2020).“Lipid profile changes during the menopausal transition,” Menopause, vol. 27, no. 7, pp. 780–787, doi: 10.1097/ GME. 0000000000001532.
[16] P. Anagnostis et al.,.(2020).“Menopause symptom management in women with dyslipidemias: An EMAS clinical guide,” Maturitas, vol. 135, pp. 82–88, doi: 10.1016/j.maturitas.2020.03.007.
[17] M. I. H. Fonseca, I. T. Da Silva, and S. R. G. Ferreira,.(2017). “Impact of menopause and diabetes on atherogenic lipid profile: Is it worth to analyse lipoprotein subfractions to assess cardiovascular risk in women?,” Diabetol. Metab. Syndr., vol. 9, no. 1, pp. 1–13, doi: 10.1186/s13098-017-0221-5.
[18] R. Zuhair and B. Hameed,.(2015).“Prolactin is a Novel Biochemical Marker in Sera of Iraqi Type-2 Diabetic Women With Metabolic Syndrome in Baghdad . Prolactin is a Novel Biochemical Marker in Sera of Iraqi Type-2 Diabetic Women With Metabolic Syndrome in Baghdad .,” no. January,.
[19] Hussein S Z and Alsamarrai A H,.(2017).“Hormonal Disturbance in Normal and Diabetic Postmenopausal Women,” Tikrit Med. J., vol. 1, no. 1, pp. 10–15.
[20] Ajam W H. Al-Khyat T H and Al-Hamdani K j, .(2012).“Oxidative Status and Lipid Profile in Post-Menopausal Women with Diabetes Mellitus (DM) Type 2 in Babylon Governorate,” Med. J. Babylon, vol. 9, no. 2, pp. 338–348.
[21] A. M. Hadi, J. H. Yenzeel, and E. N. Salman,.(2018). “Study the effect of Type 2 diabetes melitus on level of reproductive hormones in a sample of Iraqi women,” Int. J. Sci. Nat., vol. 9, no. 1, pp. 90–3.
[22] R. Haring et al., “Positive association of serum prolactin concentrations with all-cause and cardiovascular mortality,.(2014).” Eur. Heart J., vol. 35, no. 18, pp. 1215–1221, doi: 10.1093/eurheartj/ehs233.
[23] Y. Shen et al.,.(2023).“Association of prolactin with all-cause and cardiovascular mortality among patients with type 2 diabetes: a real-world study,” Eur. J. Prev. Cardiol., pp. 1–9, doi: 10.1093/eurjpc/zwad112.
[24] N. Santoro et al.,.(2008). “Factors related to declining luteal function in women during the menopausal transition,” J. Clin. Endocrinol. Metab., vol. 93, no. 5, pp. 1711–1721, doi: 10.1210/jc.2007-2165.
[25] D. Raczkiewicz, A. Owoc, A. Wierzbińska-Stępniak, and I. Bojar,.(2018). “Metabolic syndrome in peri-and postmenopausal women performing intellectual work,” Ann. Agric. Environ. Med., vol. 25, no. 4, pp. 610–615,, doi: 10.26444/aaem/74451.
[26] A. Maduka Ignatius, C. and Nnamdi Ngozika,.(2018). “Original Research Article Open Access Serum Lipids and Glycated Haemoglobin Levels in Pre-and Post Menopausal Diabetic Subjects in Enugu Nigeria.,” Int. J. Dev. Res., vol. 08, no. Cvd, pp. 23949–23954.
[27] E. Ikekpeazu, E. Neboh, I. Maduka, F. Ejezie, and S. Ufelle,.(2009). “Menopausal syndrome: Effect on serum lipid and lipoprotein profiles,” Biomed. Res. - India, vol. 20, no. 3, p. 208,doi: 10.4103/0970-938x.54859.
[28] A. R. V., M. S. A. Khan, S. M., and J. S.,.( 2020). “A Comparative Study of Lipid Profile in Pre- and Post-Menopausal Women with and without Diabetes,” Int. J. Biotechnol. Biochem., vol. 16, no. 2, p. 83, doi: 10.37622/ijbb/16.2.2020.83-91.
[29] A. F. Kadhuim,.(2020). “Estimation of serum lipid profile among pre and post-menopausal women in baghdad city,” Medico-Legal Updat., vol. 20, no. 1, pp. 877–881, doi: 10.37506/v20/il/2020/mlu/194706.
[30] D. K. Song, Y. S. Hong, Y. A. Sung, and H. Lee,.(2023). “The effect of menopause on cardiovascular risk factors according to body mass index in middle-aged Korean women,” PLoS One, vol. 18, no. 3 March, pp. 1–10, doi: 10.1371/journal.pone.0283393.
[31] A. M. A. and A. B. M. Nema R S,.(2009). “Serum Lipid Profile In Type 2 Diabetes Mellitus In Male and Females : More Atherogenic Lipid Profile In Postmenopausal females .,” vol. 14, no.1,pp. 14–22.
[32] S. H. Ko and H. S. Kim,.(2020). “Menopause-associated lipid metabolic disorders and foods beneficial for postmenopausal women,” Nutrients, vol. 12, no. 1, doi: 10.3390/nu12010202.
[33] M. D. Kulkarni and K. F. Kammar,.(2014). “Lipid Profile in Postmenopausal women of Hubli city,” J Pub Heal. Med Res, vol. 2, no. 1, pp. 25–28.
[34] S. Antwi-Baffour, R. Kyeremeh, S. O. Boateng, L. Annison, and M. A. Seidu,.(2018). “Haematological parameters and lipid profile abnormalities among patients with Type-2 diabetes mellitus in Ghana,” Lipids Health Dis., vol. 17, no. 1, pp. 1–9, doi: 10.1186/s12944-018-0926-y.
[35] S. S. Shende, M. V. Bimanpalli, I. C. Apte, V. V. Mahajan, and H. P. Narkhede,.(2011). “Study of lipid profile and C reactive protein in pre- and post-menopausal women,” J. Clin. Diagnostic Res., vol. 5, no. 8, pp. 1544–1547.
[36] C. J. Kim, T. H. Kim, W. S. Ryu, and U. H. Ryoo,.(2000). “Influence of menopause on high density lipoprotein-cholesterol and lipids,” Journal of Korean Medical Science, vol. 15, no. 4. pp. 380–386, doi: 10.3346/jkms.2000.15.4.380.