Document Type : Research Paper
Authors
University of Baghdad- College of Science
Abstract
A simple and sensitive spectrophotometric method was developed for the determination of nitrazepam
(NZP) in pharmaceutical tablets. The method was based on oxidative coupling organic reaction of reduced NZP with
pyrocatechol in the presence of ferric sulfate to form red water soluble product with maximum absorbance at 510 nm.
The reaction conditions were studied and optimized. The linear range for the determination of NZP, and the detection
limit were 1 – 24 ىg mL-1 and 0.698 ىg mL-1, respectively. The proposed method has been applied
successfully for the determination of NZP in pharmaceutical tablets. A statistical comparison of these results with
those obtained by the British pharmacopoeia procedure using the Student t-test and variance ratio F-test shows a good
agreement and indicates no significant difference in accuracy and precision at the 95% confidence.
Keywords
Main Subjects
- C. A. Fleeger, (1992). “USAN and the USP Dictionary of Drug Names”. 447, United States Pharmacopeial Convention, Inc., USA.
- B. G. Katzung, (2001). “Basic and Clinical Pharmacology”. 366, 8th Ed., McGraw-Hill, New York.
- E. Ruiz, M. H. Blanco, E. L. Abad, and L. Hernández, (1987). Determination of nitrazepam and flunitrazepam by flow injection analysis using a voltammetric detector. Analyst, 112 (5): 697-699.
- M. E. C. Peiró, D. Bose, A. M. Domínguez, M. G. Agustí, and J. E. Romero, (2002). Direct injection micellar liquid chromatographic determination of benzodiazepines in serum. J. Chromatogr. B, 780 (2): 241-249.
- G. Hancu, A. Gáspár, and Á. Gyéresi, (2007). Separation of 1,4- benzodiazepines by micellar electrokinetic capillary chromatography. J. Pharm. Biomed. Anal., 69 (3): 251-259.
- I. Bujor, P. Marcu, and L. Roman, (1978). Separation and identification of some derivatives of 1,4-benzodiazepine by thin-layer chromatography. Clujul Med., 51 (4): 351-358; through Anal. Abstr., 38, 1980, 6E40.
- M. Bakavoli, and M. Kaykhaii, (2003). Quantitative determination of diazepam, nitrazepam and flunitrazepam in tablets using thin-layer chromatography-densitometry technique. J. Pharm. Biomed. Anal., 31 (6): 1185-1189.
- C. Pistos, and J. T. Stewart, (2003). Direct injection HPLC method for the determination of selected benzodiazepines in plasma using a Hisep column. J. Pharm. Biomed. Anal., 33 (5): 1135-1142.
- M. Wang, and C. Gonnet, (1986). Reversed-phase HPLC of benzodiazepines and quinidine. Yaowu Fenxi Zazhi, 6 (3): 142-144; through Anal. Abstr., 49, 1987, 3E39.
- 10. H. He, C. Sun, X. R. Wang, C. P. Huy, N. C. Chorfi, H. Galons, M. Thevenin, J. R. Claude, and J. M. Warnet, (2005). Solid-phase extraction of methadone enantiomers and benzodiazepines in biological fluids by two polymeric cartridges for liquid chromatographic analysis. J. Chromatogr. B, 814 (2): 385-391.
- 11. P. P. Thampi, and K. R. P. Shenoy, (1986). Simple spectrophotometric method for the determination of nitrazepam in pharmaceuticals. Indian Drugs, 23 (4): 239-241; through Anal. Abstr., 48, 1986, 6E45.
- 12. S. M. Hassan, M. Sharaf El-Din, F. Belal, and M. Sultan, (1988). Application of difference spectroscopy to the determination of some pharmaceutically important nitro-compounds. J. Pharm. Pharmacol., 40 (11): 798-800; through Anal. Abstr., 51, 1989, 3E39.
13. Anon, (1982). Spectrophotometric determination of nitrazepam. Indian Drugs, 19 (11): 462-463; through Anal. Abstr., 44, 1983, 3E40.
- 14. D. M. Shingbal, and R. M. Agni, (1983). Simple colorimetric method for estimation of nitrazepam and its dosage forms. Indian Drugs, 20 (4): 162-163; through Anal. Abstr., 45, 1983, 1E55.
- 15. D. M. Shingbal, and R. M. Agni, (1983). Novel method for estimation of nitrazepam. Indian Drugs, 20 (6): 232-234; through Anal. Abstr., 45, 1983, 3E48.
- 16. I. Popovici, V. Dorneanu, R. Cuciureanu, and E. Stefanescu, (1983). Spectrophotometric determination of some 1,4-benzodiazepines with picric acid in aprotic medium. Rev. Chim., 34 (6): 554-555; through Anal. Abstr., 46, 1984, 4E43.
- 17. I. Popovici, V. Dorneanu, R. Cuciureanu, and E. Stefanescu, (1983). Spectrophotometric determination of some 1,4-benzodiazepines with picric acid in aprotic media. Rev. Chim., 34 (7): 653-654; through Anal. Abstr., 46, 1984, 4E45.
- 18. I. Popovici, V. Dorneanu, R. Cuciureanu, and E. Stefanescu, (1984). Spectrophotometric determination of nitrazepam. Rev. Chim., 35 (3): 266-267; through Anal. Abstr., 47, 1985, 1E36.
- 19. D. S. Mangala, B. S. Reddy, and C. S. P. Sastry, (1984). Extraction spectrophotometric method for the determination of reserpine and few benzodiazepine tranquillizers. Indian Drugs, 21 (11): 526-528; through Anal. Abstr., 47, 1985, 10E9.
- 20. S. R. El-Shabouri, (1986). Spectrophotometric determination of nitrazepam in tablets. Talanta, 33 (9): 743-744.
21. M. I. Walash, M. Rizk, and A. El-Brashy, (1988). Spectrophotometric determination of chlordiazepoxide and nitrazepam. Talanta, 35 (11): 895-898.
- 22. S. M. Hassan, F. Belal, M. S. El-Din, and M. Sultan, (1988). Spectrophotometric determination of some pharmaceutically important nitro compounds in their dosage forms. Analyst, 113 (7): 1087-1089.
23. “British Pharmacopoeia on CD-ROM”, (2001). Version 5, 3rd Ed., Vol. 1, Copyright by System Simulation Ltd, The Stationery Office Ltd., London.
24. M. Q. Al-Abachi and R. S. Al-Abaidi, (2003). Spectrophotometric micro-determination of folic acid in pharmaceutical tablets via oxidative coupling with catechol and ferric nitrate. Iraqi J. of Chem., 29 (1): 41-49.
25. J. N. Miller and J. C. Miller, (2001). “Statistics and Chemometrics for Analytical Chemistry”. 4th Ed., Pearson Education Limited, London.
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