Modification of the three-dimensional method for the detection of AmpC β-lactamase in Enterobacter spp. and Escherichia coli

Mustafa Mohammed, Sirwa

doi:10.37652/juaps.2010.43915

Document Type : Research Paper

Author

Sirwa Mustafa mohammed

University of Sulaimani - College of Education

10.37652/juaps.2010.43915

Abstract

Laboratory failure in the detection of Ambler class C (AmpC) β-lactamases in Gram negative bacteria
represents a major cause for its spreading and may be associated with low therapeutic outcome. The aim of this
study was to develop a more practical and simplified laboratory technique by modifying the three-dimensional
method for the detection of AmpC β-lactamase in Enterobacter spp and Escherichia coli. Twenty eight clinical
isolates including 11 isolates of Enterobacter cloacae, 2 isolates of Enterobacter aerogenes and 15 isolates of
Escherichia coli tested by standard disk diffusion methods against cefoxitin 30μg disk as primary screening for
detection of AmpC β-lactamase then all isolates tested for its production of AmpC β-lactamase by a modified three
dimensional method (3DM) with cefoxitin. Escherichia coli ATCC 25922 was used as negative controls (non-AmpC
producer). In addition, the susceptibility test of 15 Escherichia coli isolates was performed against selected betalactam
antibiotics. All Enterobacter isolates and one Escherichia coli isolate showed resistance to cefoxitin and
showed positive result with the modified 3DM (M3DM) by enhanced growth of the surface organism (E. coli ATCC
25922) at the point where the slit intersected the zone of inhibition, while all cefoxitin-susceptible E. coli isolates
showed M3DM negative results. A high percentage of Escherichia coli isolates were non-susceptible to β-lactam
antibiotics used but they showed good susceptibility to imipenem (100%).The M3DM provides a cost-effective
alternative for AmpC β-lactamase testing in clinical microbiology laboratories, thus negating the need for tedious
bacterial lysis procedures required in the 3DM

Keywords

Main Subjects

Biology

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Journal of University of Anbar for Pure Science

Modification of the three-dimensional method for the detection of AmpC β-lactamase in Enterobacter spp. and Escherichia coli

References

References

Volume 4, Issue 2
August 2010
Page 8-12

Modification of the three-dimensional method for the detection of AmpC β-lactamase in Enterobacter spp. and Escherichia coli

References

References

Volume 4, Issue 2August 2010Page 8-12

Volume 4, Issue 2
August 2010
Page 8-12