Inhibition of swarming in Proteus mirabilis by Alum (Hydrated Aluminum Potassium Sulfate)

Tahreer Ahmed, Karama

doi:10.37652/juaps.2011.44109

Document Type : Research Paper

Author

Karama Tahreer Ahmed

AL-Anbar University- College of Dentistry

10.37652/juaps.2011.44109

Abstract

The antibacterial activity of different concentrations of Alum (Hydrated Aluminum
Potassium Sulfate) were examined against Proteus mirabilis that causes upper urinary tract infections
.During our experiments we found that the following concentrations ( 0.0, 0.05, 0.1, 0.3, 0.5, 0.6, 0.7
, 0.8, 0.9 mg/ ml ) were given gradual effect on swarm, a phenotype that was associated with
motility. These concentrations were used to inhibit swarming and motility the two important virulence
factors coupled closely with the ability to invade urothelial cells. The present study revealed that
Alum began significantly inhibited swarming at 0.3 mg/ml for all seven isolates of P. mirabilis that
tested in the study . It was considered that the concentration 0.6 mg/ml completely inhibition the
phenomenon of swarming because the bacteria appear as separated small colony on nutrient agar .
The diameter of swarming was decreased by increasing the concentration of Alum. Although it was
found that this bacteria losses the motility on semi-solid media in 0.6 mg/ ml of alum( Hydrated
Aluminum Potassium Sulfate) after 24 hrs incubation but the Minimum Inhibition Concentration
( MIC ) of Alum was 0.8 mg/ml .The study revealed that Alum has the potential effect against P.

Keywords

Main Subjects

Chemistry

References

1-Xin Li,C.Virginia Lockatell,David E.Jonson, M. Chelsea Lane,John W. warren, and Harry L.T. Mobley (2004) Development of an intranasal vaccine to prevent urinary tract infection by Proteus mirabilis . Infect Immun.72 :66-75

2-.Johnson, D. E., Russell, R. G., Lockatell, C. V., Zulty, J. C., Warren, J. W. & Mobley, H. L. (199)Contribution of Proteus mirabilis urease to persistence, urolithiasis, and acute pyelonephritis in a mouse model of ascending urinary tract infection Infect Immun 61:748–2754.

3-Warren, J. W., Damron, D., Tenney, J. H., Hoopes, J. M., Deforge, B. & Muncie, H. L., Jr (1987). Fever, bacteremia, and death as complications of bacteriuria in women with long-term urethral catheters. J Infect Dis 155: 1151–1158 .

4-Mobley, H. L. & Hausinger, R. P. (1989). Microbial ureases: significance, regulation, and molecular characterization. Microbiol Rev 53: 85–108.

5-Senior ,B.W.(1997) The ability of Proteus mirabilis strain to invade the blood stream is independent of its proticin production/proticine sensitivity type .Micribiol.46:407-412 .

6-Stickler D, Ganderton L, King J, Nettleton J, Winters C( 1993) Proteus mirabilis biofilms and the encrustation of urethral catheters. Urol Res. 21(6):407-411

7-BrianVJones,Mahenthiralingam, N A Sabbuba and D J Stickler( 2005 ) Role of swarming in the formation of crystalline Proteus mirabilis biofilms on urinary catheters . J Med Microbiol 54 , 807-813 .

8-A. E. Budding,, C. J. Ingham, W. Bitter, C. M. Vandenbroucke-Grauls, and P. M. Schneeberger ( 2009 ) The Dienes Phenomenon: Competition and Territoriality in Swarming Proteus mirabilis J. Bacterio .191( 1217): 3892-3900 . 9- Liaw, S. J., Lai, H. C., Ho, S. W., Luh, K. T. & Wang, W. B. (2000). Inhibition of virulence factor expression and swarming differentiation in Proteus mirabilis by p-nitrophenylglycerol J. Med Microbiol 49: 725–731

10- Won-Bo Wang, Hsin-Chih Lai, Po-Ren Hsueh, Robin Y.-Y. Chio (2006) Inhibition of swarming and virulence factor expression in Proteu smirabilis by resveratrol . J Med Microbiol. 55 : 1313-1321 .

11-Oneda S, Takasaki T, Kuriwaki K, Ohi Y, Umekita Y, Hatanaka S, Fujiyoshi T, Yoshida A, Yoshida H ( 1994) .Chronic toxicity and tumorigenicity studyofaluminum potassium sulfate in B6C3F1mice . In vivo 8(3):271- 278

12-Goswami AK, Mahajan RK, Nath R, Sharma SK( 1993). How safe is 1% ALUM irrigation in controlling intractable vesicle hemorrhage? J Urol 149:264-267.

13-Washington C.Winn,Jr.,Stephen D.Allen ,William M. Janda,Elmer W.Koneman, Gary W.Procop,Paul C.Scheckenberger, Gail L.Woods,(2006)Color Atlas Textbook of Diagnostic Microbiology ,sixth edition . p227-270

14-Baron,E.J.and Finegold,S.M.(1994). Microorganisms encountered in urinary tract in Baily& Scott`s Diagnostic Microbiology .(9th)ed. Mosby Company .U.S.A.p220-225

15- National Committee for Clinical Laboratory Standards. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically—Sixth Edition: Approved Standard M7-A6 (2003) Wayne, PA, USA: NCCLS .

16- Gygi D, Rahman MM, Lai HC, Carlson R, Guard-Petter J, Hughes C. A.(1995) Cell-surface polysaccharide that facilitates rapid population migration by differentiated swarm cells of Proteus mirabilis. Mol Microbiol.17(6):1167-75

17-P C Bulson, D L Johnstone, H L Gibbons and W H Funk( 1984)Removal and inactivation of bacteria during alum treatment of a lake. Appl Environ Microbiol. 48(2): 425-430.

Diseases 4( 2): 84-90.

18- Judith F. M. Hoenige. (1965).Influence of pH on Proteus Flagella. J.Bacterio 90(1): 275-279

19-Harmon, R. C., Rutherford, R. L., Wu, H. M. & Collins, M. S. (1989). Monoclonal antibody-mediated protection and neutralization of motility in experimental Proteus mirabilis infection. Infect. Immun 57 :1936–1941.

20-P C Bulson, D L Johnstone, H L Gibbons, and W H Funk .( 1984) .Removal and inactivation of bacteria during alum treatment of a lake. Appl Environ Microbiol. 48(2): 425–43

21-MSDS.Material and Safety Data sheet.MallinckrodChemicalJ.T.Bakr,Inc.(2009) Aluminum Potassium Sulphat

22- Cheong Hian Goh, Paul Wan Sia Heng, Esther Pei En Huang, Benny Kai Hui Li and Lai Wah Chan .(2008) Interactions of antimicrobial compounds with cross-linking agents of alginate dressings . J. Antimicro. Chemoth. 62(1):105-108

Journal of University of Anbar for Pure Science

Inhibition of swarming in Proteus mirabilis by Alum (Hydrated Aluminum Potassium Sulfate)

References

References

18- Judith F. M. Hoenige. (1965).Influence of pH on Proteus Flagella. J.Bacterio 90(1): 275-279

19-Harmon, R. C., Rutherford, R. L., Wu, H. M. & Collins, M. S. (1989). Monoclonal antibody-mediated protection and neutralization of motility in experimental Proteus mirabilis infection. Infect. Immun 57 :1936–1941.

20-P C Bulson, D L Johnstone, H L Gibbons, and W H Funk .( 1984) .Removal and inactivation of bacteria during alum treatment of a lake. Appl Environ Microbiol. 48(2): 425–43

21-MSDS.Material and Safety Data sheet.MallinckrodChemicalJ.T.Bakr,Inc.(2009) Aluminum Potassium Sulphat

Volume 5, Issue 2
August 2011
Page 20-24

Inhibition of swarming in Proteus mirabilis by Alum (Hydrated Aluminum Potassium Sulfate)

References

References

18- Judith F. M. Hoenige. (1965).Influence of pH on Proteus Flagella. J.Bacterio 90(1): 275-279

19-Harmon, R. C., Rutherford, R. L., Wu, H. M. & Collins, M. S. (1989). Monoclonal antibody-mediated protection and neutralization of motility in experimental Proteus mirabilis infection. Infect. Immun 57 :1936–1941.

20-P C Bulson, D L Johnstone, H L Gibbons, and W H Funk .( 1984) .Removal and inactivation of bacteria during alum treatment of a lake. Appl Environ Microbiol. 48(2): 425–43

21-MSDS.Material and Safety Data sheet.MallinckrodChemicalJ.T.Bakr,Inc.(2009) Aluminum Potassium Sulphat

Volume 5, Issue 2August 2011Page 20-24

Volume 5, Issue 2
August 2011
Page 20-24