Document Type : Research Paper
Authors
University of Mosul - College of Education.
Abstract
A simple, rapid and sensitive spectrophotometric method for the determination of
noradrenaline was developed. The method is based on the proton transfer reaction with quinalizarin in
aqueous neutral solution to form a violet product showing maximum absorbance at 560 nm with molar
absorptivity of 6680 l.mol-1.cm-1. The method follows Beer’s law over the concentration range
(5.91×10-6 -5.91×10-5) mol.L-1 The accuracy (average recovery) of the method is 99.72% and the
precision (RSD) of the method is less than 1.5%.The method was successfully applied for the
determination of noradrenaline in pharmaceutical formulation as injection and the results were in a
good agreement with the standard addition procedure.
Keywords
Main Subjects
- G.E. Morgan, Jr. , MD Majed S. Mikhail, MD, (2006), “Clinical Anesthesiology”, 2nd Ed. , P.249.
- Zang D., Min Fu, Wanyun M.A. and Chen D., Anal. Sci., 17, 1331, (2001).
- Teschemacher A.G., Autonomic Neuroscience: Basic and Clinical, 117, 1-8, (2005).
- Deftereos N.T., Calokerinos A.C. and Efstathiou C.E., Analyst, 118, 627, (1993).
- Min Zhu, Xuemei Huang, Jie Li and Hanxi Shen, Anal. Chim. Acta, 357 (3), 261 (1997).
- Barnwn W.D., Anal. Chim. Acta, 89(1), 157, (1977).
- Sankar D.G., Sastry C.S.P., Reddy M.N. and Aruna M., Ind. J. Pharm. Sci., 50(3), 178, (1988).
- Sankar D.G., Sastry C.S.P., Reddy M.N. and Prasad S.N.R., Ind. J. Pharm. Sci., 49(2), 69, (1987).
- Salem F.B., Talanta, 34(9), 810, (1987).
10. Sorouraddin M.H., Manzoori J.L., Kargarzadeh E.K. and Haji Shabani A.M., J. Pharm. and Biomed. Anal., 18 (4,5), 877, (1998).
11. El-Kommos M.E., Mohamed F.A. and Khedr A.S., Talanta, 37(6), 625, (1990).
12. Al-Ghabsha T.S. and Al-Delymi A.M.S., (2008) spectrophotometric assay of noradrenaline in pharmaceutical formulation with alizarin red sulphonate. J. Edu. And Sci., 21, 62- 67.
13. A.M.S. Al-Delymi, (2006), “Development of Spectrophotometric Methods in Organic and Drug Analysis”, Ph.D. Thesis, Mosul University.
14. Delevie R., “Principle of Quantitative Chemical Analysis”, McGraw-Hill, New York, P.498, (1997).
15. Khranina O.V., Chernyakovskii F.P. and Denisov G.S., J. Mol. Struct., 177, 309-315, (1987); Chem. Abst., 111, 5, 38746w, (1989).
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