Document Type : Research Paper

Author

University of Anbar - College of Education for women

10.37652/juaps.2012.61723

Abstract

The study was carried out on (80) individuals of insomnia tablets (Captagon), The ages of the individuals was
between (20-40) years and the period of tablet taking was between (2-5)years . The study sample was divided into
two group (40)drivers and(40)night guards , and (20) person as control sample , The study aimed to study some side
effects of the drugs which accompany this case of addiction. The study also aimed to identify the physiological
changes which could happen to the addicts of such drugs and study the cytogenetic and molecular measurements of
the addicts . It has been found that the use of Captagon causes many side effects like (fatigue and anxiety) and a
significant decrease P average of the total W.B.Cs Count (4600 and 4100)cell/ml3 , associated with decrease of immunity of the addicts
greatly.There is a significant increase of the average of the E.S.R. P increase of the biomarkers of the liver function tests TSB ( 3.2 and 3.8)mg/dl , ALP(24 and 28)K.A.U./dl ,GOT(32
and 34)U/L and GPT (36 and 38)U/L . On the other hand there were a significant increase of the average of the
biomarkers of Kidney function tests blood urea (42 and 47)mg/dl and serum creatinine (1.4 and 1.8) mg/dl .
Although there were no chromosomal aberrations or disorders , there were formations of micronuclei of the
individuals of sample(9 and 13)% and this may refer to micro deletions breaks which could not be observed easily
through this test. Also there was an increase in the mitotic index (MI) with (3.0 and 2.7) and molecular aberrations
were forming new bands in the RAPD-PCR test (3 and 7)% of the individual of the sample.

Main Subjects

 
1- الإدمان أسبابه ومظاهره والوقایة والعلاج. الکتاب الخامس من سلسلة کتب مرکز أبحاث مکافحة الجریمة. وزارة الداخلیة,المملکة العربیة السعودیة.1406هـ (1986م).
2- جامع، حامد(2009) المخدرات أنواعها ومادتها وحکمها، وزارة الداخلیة الکویتیة، الطبعة الثانیة، ص 15 , 21.
3- Baer DM. Paulson RA. Williams RH.(2007) Cut-off and toxicity levels for drugs-of – abuse testing. Clin. Lab.pp12-13
4- World Drug Report(2009) Trends in drug use among young people ,United
Nations Office on Drugs and Crime (UNODC), United Nations Publication Sales No. E.09.XI.12 , pp 23-56
5- Pamela C. Richard A. pharmacology(2005) 2nd ed. British med. Assoc. Royal Pha. Soc. G. Britain
6. World Health Organization(2001) Manual of basic techniques for a laboratory, 1st.Ed., Geneva.
7. Martin H.(2006) Basic Methods for the Biochemical Lab,1st.Ed. Springer-Verlag Berlin Heidelberg.
8. Tracey Hopkins, BSN, RN(2005) LAB Notes guide to lab. diagnosis test ,F. A. Davis Co., Philadelphia.
9. World Health Organization(2003) Manual of basic techniques for a laboratory, 2nd ed., Geneva.
10. Hon Fong L (2000) medical Cytogenetics Madison Avenue, New York,.
11- Yaseen, N.Y. ; Hummadi, A.M.A.; Tawfik, M.S. and Rasheed, S.R. (1999). Modified technique in Cytogenetic preraration of leukaemia. J. Nah. Univ. Sience. 3: 175-180.
12-Schmid,W.(1975) The micronucleus test. Mut. Res. 31:9-15.
13-Joshi, N., R.A. Rawat, B. Subramanian and K.S. Rao(2010) A method for all genomic DNA isolation suitable for molecular marker analysis. Indian J. Sci. Technol., 3: 1214-1217.
14-Bautista, R., F.M. Canovas and M.G. Claros(2003) Genomic evidence for a repetitive nature of the RAPD polymorphism in human. Euphytica, 130: 185-190.
15-Maniatis ,T.,Fritsch,E.F. and Sambrook.(2001).In vitro applications of DNA by the polymerase chain reaction , in molecular cloning: A laboratory manual.2nd ed., Cold Spring Harbor lab.
16-Ramanathan, T., T Dhinesh, S Gurudeeban and K Satyavani(2011) In vitro screening of salt marsh plants for hemolytic activity in human blood. Inventive Rapid: Ethno Pharmacol., Vol. 2. No. 2.
17-Rahman K. (2009) Biochemical Parameters in the Serum of Neurological Patients in Bangladesh. Int J Biomed Sci. 5(4):395–401.
81- شیبتون، بارکر. الأفریقیة والمجاعات، والأمن الغذائی: أنثروبولوجیا وجهات نظر(2007) الاستعراض السنوی للأنثروبولوجیا،19: 353 -394.
19- Di Marzo V, Cote M, Matias I, Lemieux I, Arsenault BJ, Cartier A, Piscitelli F,Petrosino S, Almeras N, Despres JP(2009)Changes in plasma endocannabinoid levels in addict men following a 1 year lifestyle modification programme associations with changes in metabolic risk factors. Diabetologia, 52:213-217.
20-Vinyoles E. Vera M. Cecilia F. Blood Pressure Measurement , the Waiting Time Between Readings (2006): Pp.14.18
21- الراوی،عبد المجید عبد العزیز، سمیر مشرف خلف، دراسة وراثیة – خلویة على المدمنین على تعاطی الأدویة المسکنة (2007)، مجلة جامعة الانبار للعلوم الصرفة، المجلد الأول، العدد الأول.
22-KENNEDY, M. THURSBY.P Cortisone in tretment of children wilfi chronic asthema(2001) Med. Res. Cou. Staff; Clinical Assistant In Respiratory Physiology, Stoke-on-Trent Consultant Paediatrician, North Staffordshire Royal
23- الغانمی، یاسمین خضیر خلف. (2006). دراسة التغیرات المناعیة والوراثیة فی الأشخاص المعرضین للنفط ومشتقاته. أطروحة ماجستیر، کلیة التربیة، جامعة کربلاء.
24- عجام , رجاء مهدی وشاکر , محمد عبد الوهاب (1999). التأثیر السمی الوراثی للفینول والتلوین فی الخلایا اللمفاویة للإنسان (خارج الجسم). مجلة أبحاث الثقافة الحیویة , المجلد الأول , العدد / 1، ص 35-28.
25- الأعظمی،محمد عبد الوهاب (2000).دراسة التغیرات الکروموسومیة فی الإنسان الناتجة من التلوث بالنواتج العرضیة للصناعات النفطیة. رسالة ماجستیر، کلیة العلوم، جامعة بغداد.
26-Zhao, W. and Y. Pan(2004)Genetic diversity of by DNA markers. Int. J. Agric. Biol., 6: 950-956. 27-Moslem, M.A.; Bahkali, A.H.; Abd-Elsalam, K.A(2010) An efficient method for DNA extraction from Cladosporioid fungi. Genet. Mol. Res. 9, 2283–2291.