DETECTION OF SK GENE ENCODING STREPTOKINASE IN DIFFERENT PATHOGENIC BACTERIAL ISOLATES

K. BUNIYA, HARITH

doi:10.37652/juaps.2012.77312

Document Type : Research Paper

Author

HARITH K. BUNIYA

University of Anbar - College of Education for Pure Sciences

10.37652/juaps.2012.77312

Abstract

The aim of this study is detection of present sk gene in the different pathogenic bacteria isolates. We collected 22 bacterial isolates from different patients, by ceasinolytic assay we checked the ability of these isolates to produced the streptokinase protein, 12 isolates given positive result in this assay. The plasmid DNA isolated from that strains and used as a template in polymerase chain reaction (PCR) to amplified sk gene by using gene specific primers. From only two E. coli isolates we obtained the 1.3 kb DNA fragment and by agarose gel electrophoresis we determined the DNA fragments size compare with DNA Ladder.

Keywords

Streptokinase

Main Subjects

Biology

References

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Journal of University of Anbar for Pure Science

DETECTION OF SK GENE ENCODING STREPTOKINASE IN DIFFERENT PATHOGENIC BACTERIAL ISOLATES

References

References

Volume 6, Issue 3
December 2012
Page 25-29

DETECTION OF SK GENE ENCODING STREPTOKINASE IN DIFFERENT PATHOGENIC BACTERIAL ISOLATES

References

References

Volume 6, Issue 3December 2012Page 25-29

Volume 6, Issue 3
December 2012
Page 25-29