Diagnostic value of Anti-CCP antibodies compared with Rheumatoid factor in Rheumatoid arthritis patients.

N. Abdullah, Hanaa; N.Al-Thuwani, Amina; I. Nadi, Mohamed; Al-Badri, Khudhair

doi:10.37652/juaps.2012.78249

Document Type : Research Paper

Authors

¹ Foundation of technical education - College of Health and medical technology

² University of Baghdad- Institute Genetics Engineering and biotechnology for Postgraduate .

³ University of Baghdad - College of Medicine.

10.37652/juaps.2012.78249

Abstract

Citrullinated proteins have been discovered in the joints of patients with rheumatoid arthritis but not in other forms of joint disease. The citrullinated proteins in the joints correspond to the presence of the citrulline antibodies in the blood and suggest a possible role for these antibodies in the development of rheumatoid arthritis. The present study assessed the recent diagnostic value of anti-cyclic citrullinated antibodies (CCP) compared with rheumatoid factor (RF) in patients with rheumatoid arthritis. One hundred blood samples were collected from RA patients and thirty from apparently healthy group. Sera from each subject were tested for anti- CCP and RF by enzyme linked immunosorbent assay (ELISA). The majority of patients were females (84%), with a female: male ratio 5.2:1. The results indicated that anti-CCP postivity for RA patients was (69%) when compared with the healthy group (0.0%), which was highly significant in RA in comparison with control groups (P<0.001). The current study revealed that the sensitivity and specificity of Anti-CCP appeared the highest (69%) in comparison with low (47%) sensitivity for RF. Moreover, the specificity of Anti-CCP was very high (100%), while the specificity of RF was high (90%).

Keywords

Main Subjects

Biology

References

1.Smolen, J. (1996). Autoantibodies in rheumatoid arthritis. In: Manual of Biological Markers of Disease. Edited by van Venrooij ,W.& Maini ,R. Section C 1. Dordrecht: Kluwer Academic Publishers. PP:1-18.

2.Pai, S.; Pai, L. & Birkenfeldt, R. (1998). Correlation of serum IgA rheumatoid factor levels with disease severity in rheumatoid arthritis. Scand J. Rheumatol.;27 : 252–256.

3.Houssien, D.;Jonsson, T.; Daview, E. & Scott, D. (1998). Rheumatoid factor isotypes, disease activity and the outcome of rheumatoid arthritis. Comparative effects of different antigens. Scand J. Rheumatol. ;27 : 46–53.

4.Steiner ,G. and Smolen , J. (2002) . Autoantibodies in rheumatoid arthritis and their clinical significance. Arthritis Res.; 4 Suppl 2S:15.

5.Vossenaar, E.; Zendman, A.; Van Venrooij, W. & Pruijn G. (2003). PAD, a growing family of citrullinating enzymes: Genes, features and involvement in disease. Bioessays; 25:1106–1118.

6.Schellekens, G. ; De Jong, B.;Van den Hoogen, F.; Van de Putte ,L. & Van Venrooij ,W. (1998). Citrulline is an essential constituent of antigenic determinants recognized by rheumatoid arthritis specific autoantibodies. J. Clin .Invest.; 101:273-281.

7.Rantapaa-Dahlavist, S. (2005). Diagnosis and prognostic significance of antibodies in early rheumatoid arthritis. Scand. J. Rheumatol.; 34: 83-96.

8.Rantapaa-Dahlqvist, S.;De Jong, B.; Berglin, E.; Hallmans, G.;Wadell, G.; Stenlund, H.; Sundin, V. & van Venrooij, W. (2003). Antibodies against Cyclic citrullinated peptide and IgA rheumatoid factor predict the development of Rheumatoid arthritis. Arthritis Rheum.;48: 2741-2749.

9.Silman, A. & Pearson , J. (2002). Epidemiology and genetics of rheumatoid. Arthritis Res.; 4 Suppl : S265–S272.

10.Pruijn, G.; Vossenaar, E.; Drijfhout,J.; Van Venrooij, W. & Zendman, A. (2005). Anti-CCP Antibody detection Facilitates early diagnosis and prognosis of rheumatoid arthritis. Current Rheum. Reviews ; 1:1-7.

11.Abbas, NR. (2003). Some crucial immunological elements in patients with rheumatoid arthritis. M.Sc. thesis. College of Medicine. University of Baghdad.

12.Bas, S.; Genevay, S.; Mayer,O. & Gabay, C. (2003). Anti-cyclic citrullinated peptide antibodies, IgM and IgA rheumatoid factors in the diagnosis of rheumatoid arthritis. Rheumatol.; 42: 677-80.

13.Jonsson,T. & Valdimarrson, H. (1993). Is measurement of rheumatoid factor isotypes clinically useful. Ann.Rheum. Dis.; 52(2):161-4.

14.Vallbracht, I. ;Rieber, J.; Oppermann, M.; Forger, F.;Siebert, U. & Helmke, K. (2009). Diagnostic and clinical value of anti-cyclic citrullinated peptide antibodies compared with rheumatoid factor isotypes in rheumatoid arthritis. Ann. Rheum. Dis. ; 63: 1079-1084.

15.Quinn, M.;Gough, A.; Green, M.; devlin, J.; Hensor,E.; et al.(2006). Anti-CCP antibodies measured at disease onset help identify seronegative rheumatoid arthritis& predictor radiological and function outcome. Rheumatol.;45 (4):478-480.

16.Khosla, P.; Shankar, S. & Duggal, L. (2004). Anti-CCP antibodies in rheumatoid arthritis. J. Indian rheumatol.Assoc.;12: 143-46.

17. Nell, V.; Machold, K.; Hueber, W. et al. (2003). The diagnostic significance of autoantibodies in patients with very early rheumatoid arthritis. Arthritis Res. Ther.; 5(suppl1):16.

18.Tofiq, D. (2007). Diagnostic value of anti-CCP 2 antibodies in comparison with IgM & IgA rheumatoid factor in rheumatoid arthritis. Board for pathology /Microbiology & Immunology. The scientific council of pathology.

19.De-Rycke, L.; Peen, I.; Hoffman, I.; Kruithof, E.; Union, A.; etal.(2004). Rheumatoid factor & Anti-CCP antibodies in Rheumatoid arthritis: diagnostic value, associations with radiological progression rate & extra-articular manifestations. Ann. Rheum. Dis.; 63: 1587-93.

20. Zandman, A.;Van-venrooij, W. & Pruijn, G. (2006). Use & significance of anti-CCP auto antibodies in rheumatoid arthritis.Rheumatol.;45:20-25.

21. Bizzaro, N.; Mazzanti, G.; Tonutti, E.;Villalta, D. & Tozzoli, R. (2001). Diagnostic accuracy of anti-CCP assay for rheumatoid arthritis. Clin.Chem.; 47(6):1089-93.

22. Kroot, E.; de jong, B.; van Leeuwen, M.;Swinkels, H.;van den Hoogen,F.;et al. (2000). The prognostic value of anti-cyclic citrullinated peptide antibody in patients with recent-onset rheumatoid arthritis. Arthritis Rheum.; 43(8): 1831-5.

23.Bas, S.; Genevay, S.; Mayer,O. & Gabay, C. (2003). Anti-cyclic citrullinated peptide antibodies, IgM and IgA rheumatoid factors in the diagnosis of rheumatoid arthritis. Rheumatol.; 42: 677-80.

Journal of University of Anbar for Pure Science

Diagnostic value of Anti-CCP antibodies compared with Rheumatoid factor in Rheumatoid arthritis patients.

References

References

Volume 6, Issue 3
December 2012
Page 1-6

Diagnostic value of Anti-CCP antibodies compared with Rheumatoid factor in Rheumatoid arthritis patients.

References

References

Volume 6, Issue 3December 2012Page 1-6

Volume 6, Issue 3
December 2012
Page 1-6