Document Type : Research Paper

Author

University of Anbar -College of Education for pure science.

10.37652/juaps.2012.78441

Abstract

A bacterium that was capable of metabolizing C1- compounds was isolated and identified in Moscow State Academy of Fine Chemical Technology (Russia), as Pseudomonas sp. LP1 based on detailed taxonomic studies. This organism is a new strain of obligate methylotrophic bacteria, which was cultured on the methanol-containing medium (1%) as its sole carbon source. This study included culture of Pseudomonas sp. LP1 in M9 modified medium. The lipids were extracted from biomass of bacteria by organic solvents where a mixture of fatty acid methyl esters (FAMEs) was obtained. Antibacterial activity of total fatty acids was tested against some standard strains of both gram positive and gram negative bacteria by using wells in agar plates. Fatty acids mixture were found to have antibacterial effects. Thin-layer chromatography was used to tentatively identified the mixture components of fatty acids. A gas-liquid chromatographic system referred that Palmitic (16:0), Palmitoleic (16:1) and Oleic (18:1) acids were present.

Keywords

Main Subjects

1- Höfer P., Choi Y. J., Osborne M. J., Miguez C. B., Vermette P., and Groleau D. (2010). Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains. Microbial. Cell Factories,Vol.9, No.1 P. 70.
2- Chistoserdova L.,  Kalyuzhnaya M. G. and Lidstrom M. E. (2009). The Expanding World of Methylotrophic Metabolism. Annu. Rev. Microbiol., Vol. 63, P. 477-499.
3- Schrader J., Schilling M., Holtmann D., Sell D., Filho M. V., Marx A. and Vorholt J. A. (2009). Methanol-based industrial biotechnology: current status and future perspectives of methylotrophic bacteria. Trends Biotechnol., Vol. 27, No. 2, P. 107-115.
4- Trotsenko Y. A., Doronina N. V. and Khmelenina V. N. (2005). Biotechnological potential of methylotrophic bacteria: a review of current status and future prospects. Prikl. Biokhim. Mikrobiol., Vol. 41, No. 5, P. 433-441.
5- Hu F., Li X., Lü J., Mao P. H., Jin X., Rao B., Zheng P., Zhou Y. L., Liu S. Y., Ke T., Ma1 X. and Ma1 L. X . (2011). A visual method for direct selection of high producing Pichia pastoris clones. BMC Biotechnology, Vol. 11, No. 1, P. 23.
6- Brautaset T., Jakobsen Ø. M., Josefsen K. D., Flickinger M. C. and Ellingsen T. E. (2007). Bacillus methanolicus: a candidate for industrial production of amino acids from methanol at 50 degrees C. Appl. Microbiol. Biotechnol. 2007 Vol. 74, No., P. 22-34.
7- Dawood K. F. (2002). Biosynthetically obtained deuterated fatty acids and exopolysaccharides by using utilizing mixed culture of bacteria. P. 100., M.Sc. Thesis, MSACT. Moscow, Russia.
8- Gómez-Brandóna M., Loresb M. M. and Domínguez J. (2010).  A new combination of extraction and derivatization methods that reduces the complexity and preparation time in determining phospholipid fatty acids in solid environmental samples. Bioresource Technology, Vol. 101, No. 4, P. 1348-1354.
9- Zainuddin N. J., Babji A. S. and Said M. (2011). Extraction of lipids and purification of linoleic acid from Clarias macrocephalus oil. AACL Bioflux, Vol. 4, No. 3, P. 423-429.
10- Perez C., Paul M., Bazerque P. (1990). An Antibiotic assay by the agar well diffusion method. Acta. Bio. Med. Exp., Vol. 15, P. 113-115.
11- Sangeetha D. and Stella D. (2012). Screening of Antimicrobial Activity of Vetiver Extracts against Certain Pathogenic Microorganisms. IJPBA, Vol. 3, No. 1,      P. 197-203.
12- Christie W. W. (1993). In Advances in Lipid Methodology – Two.  pp. 195-213., Ed. W.W. Christie, Oily Press, Dundee. Scotland.
13- Christie W. W. and Han X. (2010). Lipid Analysis - Isolation, Separation, Identification and Lipidomic Analysis. 4th edition, Oily Press, Bridgwater, U.K.
14- Doronina N. V., Lee Ts. D., Ivanova E. G. and Trotsenko Y. A. (2005). Methylophaga murata sp. nov.: a Haloalkaliphilic Aerobic Methylotroph from Deteriorating Marble. Mikrobiologiia., Vol. 74, No. 4, P. 511-519.
15- Doronina N. V., Darmaeva T. D. and Trotsenko Y. A. (2003). Methylophaga alcalica sp. nov., a novel alkaliphilic and moderately halophilic, obligately methylotrophic bacterium from an East Mongolian saline soda lake. Int. J. Syst. Evol. Microbiol., Vol. 53, No. 1, P. 223-229.
16- Goglevaa A. A., Kaparullinaa E. N., Doroninaa N. V. and Trotsenko Y. A. (2011). Methylobacillus a rboreus sp. nov., and Methylobacillus gramineus sp. nov., novel non-pigmented obligately methylotrophic bacteria associated with plants. Systematic and Applied Microbiology, Vol. 34, P. 477-481.
17- Kim H. G., Doronina N. V., Trotsenko Y. A. and Kim S W. (2007). Methylophaga aminisulfidivorans sp. nov., a restricted facultatively methylotrophic marine bacterium. Int. J. Syst. Evol. Microbiol., Vol. 57, P. 2096-2101.
18- Ramachandran A. and Natarajan D. (2010). Antibacterial Activity of Gymnema kollimalayanum, A New Plant from Peninsular India. Advan. Biol. Res., Vol. 4, No. 6, P. 292-295.
19- Huang C. B., George B. and Ebersole J. L. (2010). Antimicrobial activity of n-6, n-7 and n-9 fatty acids and their esters for oral microorganisms. Arch. Oral. Biol., Vol. 55, No. 8, P. 555-560.
20- Desbois A. P. and Smith V. J. (2010). Antibacterial free fatty acids: activities, mechanisms of action and biotechnological potential. Appl. Microbiol. Biotechnol., Vol. 85, P. 1629-1642.