Document Type : Research Paper

Authors

University of Anbar - College of Science

Abstract

The study included the neutralization of bacterial plasmids E.coli and the role plasmids bacteria in response to some antibiotics . Growth of bacteria was carried out in different temperatures (40-60)0c and for different time periods (5-30) minutes to reach the 93% rate of killing and deportation in Agaros Gel Electrophoresis was detected over the losses of bacteria E.coli for plasmids compard with the original isolation .Tthe sensitivity of the bacteria was tested by discks method to antibiotics for the isolates curing compard with the original isolation . the rasults showed variation in the response of isolates to antibiotics curringcomparde with the original isolates as an antibiotic Erythromycin which become sensitive isolates by the largest rate of 31.00 mm of isolation 65 the curringwhile 0.00 mm for original isolate, and these curring isolates have retained their resistance to sodium chloride NaCl.

Keywords

Main Subjects

1.Carter, G.R.; Chengappa, M.M; Robert, A. W.; William, C. G. and Yasuk,o.R. (1995). Essentials of Veterinary Microbiology.5th ed. AwaverlyCompany.
2.Koneman, E.W.; Allen, S.D.; Jauda, W.M.; Schrechenberger, R.C. and Winn, W.S. (1997). Color Atlas and Textbook of Diagnostic Microbiology. 5th ed. Lippincott-Raven Published, Philadelphia. U.S.A.
.3شکارة، مکرم ضیاء. (2009). علم الوراثة. دار المسیرة للنشر والتوزیع والطباعة. عمان.
4.Jawetz, E.; Melinck, J.L.; Adelberg, E.A.; Geo, F.B.; Janet, S.B.; Karen, C.C.; Stphen, A.M.(2007). Jawetz, Melinck and Adelberg "Medical microbiology". 24th ed. Prentice-Hill Companies. Inc.USA.
.5کوفمان، ف. (1985). البکتریا المعویة. مترجم. وزارة التعلیم العالی والبحث العلمی. جامعة بغداد. العراق.
6.Lennette, E.H.; Balows, A.; Hausler, W.J.; Shadomy, H.J. (1985). Manual of Clinical Microbiology. 4th ed. Washington.D.C.
7.Gyles, C.L.(2007). Shigatoxin-producing Escherichia coli. J. Am. Sci. 85:45-62.
8.James, P.N. and James, B.K.(1998). Diarrheagenic Escherichia Coli. Clin. Microbiol. Rev. 11 (1):142-201.
9.Alain, L.S.(2005). Mechanism by which the disease is thought to be induced: ETEC, EPEC, EHEC, EAEC, DACE, EAEC. Clin. Microbiol. Rev. 18(2): 264-292.
10.Wegner, H.C.; Aarestrup, F.M.; Gerner – Smidit, P. and Bager, F. (1999). Transfer of antibiotic resistant bacteria from animals to man. Acta Vet. Scand. 92(1): 51 – 57.
11.Lambie, N.; Negleka, M.; Brown, G. and Ryan, J. (2000). Retrospective Study on Escherichia coli infection in Broilers subjected to postmortem examination and antibiotic resistance of isolates in Trinidad. Avian Dis. ,44 (4): 155 – 160.
12.Kuenkates, E. and Kocazeybek, B.(2002). High resistance rate against 15 different antibiotic in aerobic gram-negative bacteria isolates of cardiology intensive care unit patient. Indian. J.Microbial. 20:208-210.
13.Ang, J.Y. ; Eziike, E. and Asmar, B.(2004). Antibacterial resistance. Indian. J. Pedit. 71:229-239.
.14الطائی، هیام عادل.(2000). مقاومة البکتیریا للمضادات الحیویة وإنتاج أنزیمات بیتا لاکتامیز. مجلة الدواء العربی.( 2):16-15.
15.Shingler, V.and Thomas, C.M. 1989. Analysis of nonpolar insertion mutations in the trfA gene of IncP plasmid RK2 which affect its broad-host-range property. Published by Elsev.Sci. B.V. 1007 (3): 301-308.
16.Easter, C.L.;Schwab, H. and Helinski, D.R. (1998). Role of the parCBA operon of the broad-host-range plasmid RK2 in stable plasmid maintenance. J. Bacteriol. 180(22): 6023-6030.
17.Brinkman, F.S.L.; Schoofs, G.; Hancock, R.E.W. and DeMot, R. (1999). J. Bacteriol. 181(6):4746-4754.
18.Dupray, E.; pommepuy. M.;Derrien, A.; Caprais, M, P. and cormier, M. (1993). Use of the direct viable count (D.V.C) For the assessment of survival of E.coli in marine Environments Water.Sci. Tech. 27 (3-4): 395-399.
.19کوداینوف، اورسولا.(1985). علم الوراثة. الجزء الثانی، جامعة هارفارد. مترجم. جامعة الموصل
 20.Trevors, J.T. (1986). Plasmid curing in bacteria fems. Microbiol. Rev. 32 (3-4): 149 – 157.
21.Motallebi, M.; Zamani, M.R. and Saffar, B. (2000). Serological and Biochemical Characteristics of Virulence Plasmid of Yersinia enterocolitica Isolates from Chicken in the Islamic Republic of Iran, Eastern. Med Health J. (6): 1-6.
.22الراشدی، دیانا نور الدین مصطفى عبدالله. (2002). تحیید المحتوى البلازمیدی لجرثومة الـ Proteus mirabilis المعزولة من حالات مرضیة مختلفة فی الانسان باستخدام مواد کیمیائیة وعوامل فیزیائیة. رسالة ماجستیر _ کلیة التربیة_ جامعة الموصل. العراق.
.23حداد، جاسب جاسم.(1991). علم الاحیاء المجهریة البیطریة، اساسیات علم الجراثیم. مطبعة دار الحکمة للطباعة والنشر. الموصل. العراق.
24.Tomoeda, M.; Inuzuka, M.; Auto, Sh. and Konishi, M. (1974). Curing action of sodium dodecy sulfat on proteus mirabilis R+ strain. J. Bacteriol. 120(3): 1158 – 1162.
25.Mesas, J. M. ; Carmen-Rodriguez, M. and Teresa, T. M. (2004). Plasmid curing of Oenococcus oenis. J. Bacteriol 51(1):37-40.
26.Senior ,B.W.(1996) Examination of water ,milk food and air. Medical Microbiology. Churchill Livingston. New York.
27.Forbes,B.A.;Seham, D.F.;and Weissfeld,a.s.(1998).Bailey and Scotte Diagnosis Microbiology, 10 th ed. Mosby, Inc, St. Louis.
28.Bauer, A.W.; Kirby, W.M.; Sherris, J.C.; and Turch, M. (1966). Antibiotic susceptibility testing by standardized disk method. Aln. Clin. Pathol.,45:493-496.
29.American Public Health Association. (APHA).1998. Standard method for the examination of water and wastewater. 20th ed.
30.Sonstein, S.A. and Baldwin, J.N. (1972). Loss of the penicillinase plasmid after treatment of Staphylococcus aureus with sodium dodecyl sulphate. Bacteriol.109(1):262-265.
 31.Vreeland, R.H. (1987). Mechanisms of halotolerance in microorganisms Crig. Microbiol. Rev. 14(4): 311-356.
32.Fernandez-Castillo, R.;Vargas, C.; Nieto, J.J.; Ventosa, A. and Ruiz-Berraquero, F.(l992). Characterization of a plasmid from moderately halophilic eubacteria. J. Gen. Microbiol. 138(6): 1133-1137.
33.Tamai,E.; Shimamoto,T.; Tsuda,M.; Mizushima,T., and Tsuchiya,T. (1998). Conversion of Temperature-sensitive to –resistant Gene Expression Due to Mutations in the Promoter Region of the Melibiose Operon in Escherichia coli. J. Bio Chemi. 273(27): 16860-16864.
34.AL-Sorchee, S.M.(2009). Causative agents of diarrhoea in Erbil children and the effect of some plant extract on bacterial isolates. Ph.D. Thesis. Collage of Education (Ibn AL-Haitham). Baghdad University.
.35المقدادی، إیلاف أسامه محمد علی.(2000). دراسة کیموحیویة ووراثیة لبعض عزلات  Escherichia coli المنتجة لأنزیم البنسلینیز. رسالة ماجستیر_ کلیة العلوم_ الجامعة المستنصریة.العراق.
 36. Drlica, K. and Zhao, X.L. (1997). DNA gyrase topoisomerase IV and the4-quinolones. Microbiol.Rev.16:377-392.
37.Schmitz.F.J.; Jones, M.E.; Hofman, B.; Hansen, B.; Scheuring, S.; Luckefahr, M.; Fluit, A.C.; Verhoef, J.; Hadding, U.; Heinz, H.P. nd Kohrer, K. (1998). Characterization of grlA, grlB, gyrA and gyrB mutations in 116 unrelated isolates of Staphylococcus aureus in relation to minimal inhibitory concentrations of Ciprofloxacin. J.Antimicrob. Chemother. 42:1249-1252.
38.Tenover, F.C.; Phillips, K.L.; Gilbert, T.; Lockhart, P.; O'Hara, P.J. and Plorde, J.J.(1989). Development of DNA probe from the deoxyribonucleotide sequence of a 3-N-aminoglycoside acetyle teansferase[AAC(3)-I] resistance gene. J.Antimicrob. Chemother. 33 : 551 - 559.
39.Sherley, M.; Gordon, D.M. and Collignon, P.J. (2000). Variations in antibiotic resistance profile in Enterobacteriaceae isolated from wild Australian mammals. Environ. Microbiol. 2(6) : 620-631.
40.Jin, D.J. and Cross, C.A. (1988). Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance. J. Mol. Biol. 202(1):45-58.
41.Tomasz, A.; Drugeon, H. B.; de Lencastre, H. M.; Jabes, D.; McDougall, L. and Bille, J. (1989). New mechanism for methicillin resistance in Staphylococcus aureus: clinical isolates that lack the PBP2a gene and contain normal penicillin-binding protein with modified penicillin-binding capacity. J. Antimicrob. Chemother. 33:1869-1874.
42.Barg, N.; Chambers, H. and Kernodle, D. (1991). Borderline susceptibility to antistaphylococcal penicillins is not conferred exclusively by the hyperproduction of beta-lactamase. J. Antimicrob. Chemother. 35:1975-1979.
43.Fluit, C.; Visser, M.R. and Schmitz, F. (2001). Molecular detection of antimicrobial resistance. Clin. Microbiol. Rev. 14(4):836-
44.Luna, V.A.; Coates, P.; Eady, E.a.; Cove, J.H.; Nguyen, T.T. and Roberts, M.C. (1999). A variety of gram – positive bacteria carry mobile mef genes. J. Antimicrob. Chemother. 44(1):19-25.
45.Schmitz, F.J.; Sadurski, R.; Kray, A.; Boss, M.; Geisel, R.; Kohrer, k.; Verhoef, J. and Fluit, A.C.