Journal of University of Anbar for Pure Science

عزلت البکتریا Lactococscu lactis من عینة حلیب ماشیه وشخصت اعتمادا على خصائصها الزرعیه على وسط Man Rogosa Sharp وخصائصها المجهریه وتفاعلاتها الکیموحیویه 0 اسفرت الفحوصات التشخیصیه الزرعیه عن کون البکتریا ذات مستعمرات صغیره ذات حافات ملساء بیضاء اللون على الوسط Man Rogosa Sharp واظهرت الفحوصات المجهریه عن کون البکتریا کرویة الشکل موجبه لصبغة کرام واثبتت التفاعلات الکیموحیویه عن کونها سالبه لاختبار الکاتالیز والاوکسدیز 0لدراسة تاثیر درجة الحراره على المحتوى البروتینی للبکتریا نمیت البکنریا عند ثلاث درجات حراریه 45oC , 37oC , 28oC ثم عزلت البروتینات عند کل معامله حراریه ورحلت کهربائیا على هلام متعدد الاکریل اماید واسفرت عملیة الترحیل عن ظهور حزمه بروتینیه اضافیه عند درجة حرارةC 37o فضلا عن البروتینات التی احتوت علیها خلیة البکتریا 0

One hundred and sixty five mid stream urine specimens were collected from outpatients presented with urinary tract infections (UTI). The results showed the dominance of Escherichia coli over other causative agents. Antibiotic sensitivity test was carried out to E. coli isolates. Thence, the isolate that developed the highest multidrug resistance was chosen for further studies. Moreover, five Lactobacillus isolates comprising L. acidophilus L1 and L. acidophilus L2 were isolated from yogurt and vinegar, respectively, L. plantarum L3 and L. plantarum L5 from saliva and raw milk, respectively, while L. fermentum L4 was isolated from vagina. Cup assay method was employed to investigate the inhibitory (antagonistic) activity of lactobacilli isolates against E. coli A99 on MRS agar. Results showed that L. acidophilus L1 developed the highest activity. The cell free supernatant of lactobacilli developed the same activity. L. acidophilus L1 supernatant showed the highest inhibition activity. The present study also revealed this activity in vivo by injecting a group of mice with L. acidophilus L1 suspension or its infiltrate 30 min after injecting the E. coli A99 intraurethrally and the histopathological sections revealed the disappearance of inflammation signs caused by E. coli A99 when it was injected alone.

استخدمت فی الدراسة 112 فاراً أبیض سوسریاً لدراسة تأثیر کلورید الکوبلت على نسیج الخصیة، قسمت الى مجموعات اساسیة وثانویة وفقاً للتراکیز والجرع المستخدمة، وحضرت منها شرائح نسیجیة وتم صبغها بالصبغة الروتینیة (الهیماتوکسلین - ایوسین) لغرض فحصها ودراستها مجهریاً ، واظهرت مقاطع الخصى تغیرات نسیجیة مرضیة واضحة وازدادت بزیادة التراکیز والجرع المستعملة. حیث لوحظ انتشار السائل الوذمی (Oedemo) وحدوث نزف دموی (Haemorrhage) واحتقان الاوعیة الدمویة وکذلک انفصال الظهارة المنویة (Seminiferous epithelium) وتبعثر النسیج البینی (Interstitum) وضمور خلایا لیدک (Leydig cells) ووجود الخلایا العملاقة متعددة النوى Giant cells) (Multinuclitated والحطام النخری (Nectrotic debris)، مما یدل على حدوث تأثیرات سامة وتنخر (Necrosis) حاد للانسجة وموت الخلایا المسؤلة عن نشأة النطف (خلایا سرتولی) واسلاف النطف والخلایا النطفیة الاولیة والثانویة وطلائع النطف فی نسیج الخصى (Sertole. Cells Spermatogonia, pri & Sec spermatocytes, Spermatede).

Tilmicosin was administered to sheep intravenously and subcutaneously to determine its concentration in blood, tissue and milk and its kinetic behaviour. After a slow intravenous and subcutaneous injection, the serum concentration-time curve indicated a two compartment open model , elimination half-life (t1/2β) of (4•36 ± 0•04) hours. After a subcutaneous injection the peak plasma drug concentration was (22.54 ± 0.62) mg/ml (Cmax) at 0.5h (Tmax) and the drug was rapidly absorbed and slowly eliminated (t1/2β: 5•02 ± 0•08) h. The apparent volume of distribution of tilmicosin was more than (1.89 ± 0.041). Tilmicosin residues found in liver, kidney and tissue after the 14 and 28 day of withdrawal after intravenous and subcutaneous respectively. Tilmicosin was extensively secreted into milk, Tilmicosin was detectable in milk for 5 and 28 days after a single dose intravenous and subcutaneous respectively

Acute appendicitis is the most common acute surgical condition of the abdomen. The most
important step for appendicitis is the obstruction the eppeniceal lumen. Our study was aimed to study
the histological parameter associated with appendicitis. This study was included 55 of appendix
specimens, which were resects. These samples were collected from teaching Ramadi hospital. Gross
examination for diameter of lumen and thickness of mucosa was performed and then the samples were
fixed in 10℅ formalin for histological examination. The relation of age and sex with appendicitis was
studied in this work. This study was recorded increase in thickness of mucosa(Mean=1.4mm) and
decrease in diameter of lumen(Mean=1mm). increase incidence of appendicitis between males and
females(50.9/49%).The age of the patients ranged from (8-45)years with mean (21.4).Histological
study was revealed increase infiltration of white blood cell within muscularis mucosa. Congestion,
destruction of mucosa, hyperplasia of lymph nodules, obstruction of lumen with found some of warm
such as Enterobious vermicularis within lumen. From these results we found the ratio of incidence of
appendicitis increase in males than females and ensured that by increase the histological parameters
and pathological changes compare with normal

This study was done in Fallujah Hospital and AL-Jameaa medical Lab in Fallujah city since the beginning of February of 2009 until June 2009. Hormonal changes in the serum of polycystic ovary syndrome (PCOS) infertile women in Fallujah city and there countryside’s were studied. Forty infertile women affected with increase of testosterone were selected, twenty of them with (PCOS) compared with twenty normal women. All of the women in this study from Fallujah city and surrounded area with different ages. All women showed the same symptoms, like hair growth in face, bold voice with an increase in testosterone and increase in LH while there no differences in FSH level. The levels of Prolactin was increased at (12.5%) .In conclusion the high levels of testosterone is responsible of hirsutism studeid in women lead to presence of the secondary characters of men in women and this because of high levels of in free androgens which lead to the increase estrogen in esteron form that make a appositive feed back on LH secretion which lead to increase testosterone secretion from the ovary.

The effect of copper on some serum biochemical measurements was tested in rats
including some of the key enzymes such as Alkaline phosphatase (ALP) and Acid phosphatase (ACP)
and Aspartate Aminotransferase (AST) and Alanine Aminotransferase (ALT) and Lactate
Dehydrogenase (LDH) and Glucose - 6 - phosphate Dehydrogenase (G6PDH), in addition to measuring
of total protein . These biochemical measurements tested for each group of male rats , treatment and
control results showed significant changes in enzymatic activity and total protein compared to the
control group , where it was observed a significant decrease in the amount of total protein (P<0.05)
compared with a control , where the greater concentration of copper increased with low amount of total
protein . It was also observed a significant decrease (P<0.05) in the effectiveness of the enzyme
Alkaline phosphatase (ALP) concentrations in all treatment of male rats compared with control group.
The enzyme Acid phosphatase (ACP) was the cause of copper increased significantly (P<0.05) in the
effectiveness of this enzyme in all the different concentrations of copper as compared to control . In the
enzymes of the Aspartate Aminotransferase (AST) and Alanine Aminotransferase (ALT) , where it was
noted that increased significantly (P<0.05) in the effectiveness of the enzyme (AST) compared to
control and decrease significantly (P<0.05) the level of effectiveness of the enzyme (ALT) compared to
control . In the enzyme Lactate dehydrogenase (LDH) has been observed a significant decrease
(P<0.05) as compared to the enzyme effectively control the three transactions as a result of a copper .
In the enzyme Glucose – 6 - phosphate Dehydrogenase (G6PDH) , it was noted that increased
significantly (P<0.05) in the effectiveness of the enzyme as a result of exposure to different
concentrations of copper as compared to control .

A spectrophotometric method for the assay of trace amounts of benzocaine was based on the reaction of benzocaine with nitrite ion to form the corresponding diazonium salt followed by coupling reaction with ethyl cyano acetate to form a stable and a soluble yellow azo dye with maximum absorption at 405 nm. Beer،¦s law was obeyed over the range 5-250ƒفg/25ml, i.e, (0.2-10ppm) of benzocaine and correlation coefficient 0.998 with a molar absorptivity of 3.1 „e104 L.mol-1.cm-1, a relative error of 0.0 to 0.25 % and a relative standard deviation of „b 0.03 to „b 0.38 %, depending on the concentration level. The method has been successfully applied for the assay of benzocaine in one pharmaceutical preparation (Lozenges).

تم حساب ترددات الاهتزاز وشدد امتصاص طیف الاشعة تحت الحمراء, لجمیع الاحداثیات الداخلیة لجزیئة [6] سایکلاسین نوع (Armchair) و بعددها 3N–6)) و مناقشتها تماثلیا و تأصریا وفق نظریة دوال الکثافة (DFT) وباسلوب (B3LYP) وعناصر قاعدة (6-311G). کانت قیم ترددات الاهتزاز المحسوبة قریبة من القیم التجریبیة للعدد القلیل جدا من قیم ترددات الاهتزاز المستخرجة للانابیب المبنیة من هذا النوع من الجزیئات. و عند مقارنة نتائج الحسابات، وجد بأن ترددات الاهتزاز للانماط المتماثلة لحرکات المط التآصریة أعلى من تلک غیر المتماثلة لاواصر (C-H) والعکس لاواصر (C-C)، و تکون ترددات الاهتزاز غیر المتماثلة للحرکات الانثنائیة (CH) و (CC) أعلى من نظائرها المتماثلة. ویمکن تلخیص هذه العلاقات بالاتی: sym.CH str. > asym. CH str. و بصورة عامة نجد أن:sym CC str. < asym. CC str. C=C str. (circum.) >  C--C str. (axial.) >  C-C str. (circum.)و أن:sym (C=C str.) < asym (C=C str.) circum. sym (C--C str.) < asym (C--C str.) axial sym (C-C str.) < asym (C-C str.) circum. حیث (C-Caxial) هی اطوال اواصر کاربون کاربون المتجهه على طول المحور العمودی فی الجزیئة، اما (C-Cc) فهی اطوال اواصر کاربون-کاربون المتجهه على طول محیط الجزیئة. کما تم و بدقة تعیین جمیع ألانماط الاهتزازیة العائدة للحرکات الانبعاجیة و التنفسیة والانحنائیة باتجاه وعکس اتجاه عقرب الساعة، و التی تعود الیها التشوهات الحاصلة من جراء الاهتزاز. أیضا تم حساب و دراسة توزیع الکثافة الالکترونیة على ذرات هذه الجزیئة، و کانت النتائج متوافقة مع الصفات الفیزیاویة والتوصیلیة للانابیب المبنیة من هذا النوع من الجزیئات.

:In this research, the study shows the effect of vitamin C absorption by poly ethylene terphthalate
(PET) bottles on mechanical properties of (PET) bottles that was investigated through tensile test. The
polyethylene terphthalate bottle containing orange drinks with ( 50ppm ) initial concentration of vitamin C were
stored for about three months at ( 10, 25 , 50 ºC ) and the mechanical properties ( tensile strength , modulus of
elasticity and elongation ) of poly ethylene terphthalate bottles were measured periodly during the storage time .
The effect of vitamin C on the mechanical properties of (PET ) bottles was compared between of the (PET )
bottles containing orange drinks with (50ppm ) vitamin C and the (PET ) bottles containing orange drink without
concentration additional of vitamin C solution , maximum effect of vitamin C on mechanical properties of (PET)
bottles was observed on the samples stored at 50ºC that could be represents to the high amount of absorbed
vitamin C at high storage temperatures . It was also found that tensile strength and modulus of elasticity of
( PET ) bottles decreased with the absorption degree of vitamin C, while the elongation increased

This study was conducted to evaluate the effect of the number of cycles of cleaning using NaOCl solution of different concentration and sterilization using autoclave and dry heat oven on the torsional properties of rotary (Ti-Co) instruments. A total of forty five rotary protaper finisher instruments were divided into 3 groups: Group 1 were subjected to neither cleaning nor sterilization cycles and assigned as the control group. Group 2 and group 3 were subjected to 5 and 10 cleaning and sterilization cycles respectively, soaked within (2% and 5%) NaOCl, were sterilized with autoclave and dry heat oven. Then, all instruments were subjected to a clockwise torsional load until fracture, the torsional moment and angular deflection at fracture were calculated. The results show a significant reduction in the torsional strength after cleaning and sterilization cycles regardless the concentration of NaOCl, type and number of sterilization cycles. For the angular deflection, there was a highly significant reduction, where, the number of cycles was the most influencing factor for reduction of the angular deflection whereas, the concentration of NaOCl and the type of sterilization were show no significant influence on the angular deflection.

یتضمن البحث تحضیر سلسلة من المرکبات بطریقة ازدواج املاح الدیازونیوم مع الیوردین. وتم التأکد من الصیغ الترکیبیة للمرکبات المحضرة بدراسة اطیاف الاشعة فوق البنفسجیة (U.V) واطیاف الاشعة تحت الحمراء(I.R) والتحلیل الدقیق لعناصر الکاربون، الهیدروجین والنتروجین (C.H.N) وتضمن البحث ایضاً دراسة الفعالیة البایولوجیة لهذه المرکبات على خمسة اجناس من البکتریة المرضیة: S. aureus , Str. viridians, Ps. aeruginosa , E. coli and Sh. Dysenteriae وبأستخدام طریقة نشر الاقراص ووجد ان لهذه المرکبات تأثیر متوسط فی تثبیط هذه الاجناس من البکتیریا.

The chirality in the negative-parity levels in 105Rh nucleus has been examined by the theoretical model Interacting Boson-Fermion Model (IBFM). The IBFM succeed in prediction the negative parity excitation energy in the 105Rh nucleus. Including high-j orbits in the IBFM calculations or not gave nearly the same behavior. This nucleus could have static chirality but not vibration chirality. The negative parity bands dose not show the ideal chiral symmetry.

Vibration frequencies, IR absorption intensities and normal coordinates of the
Coronene radical cation and anion were calculated applying the semiemperical methods MINDO/3 and
PM3, and quantum mechanical method (DFT (B3LYP/6-311G)). The results allowed proper
assignments for the frequencies of the experimentally known, radical cation vibrations. They provided
preestimation of the radical anion frequencies. Comparison is done for the frequencies of the ions with
those of the neutral Coronene molecule. It was found that the C-H stretching frequencies are directly
related to the carbon -electron densities of the relevant atoms. This is not true for the change in the
CC stretching frequencies, which seem to be due to the change in symmetry from D6h for the neutral
molecule to D2h for its positive and negative radical ions

In this paper we introduce two new concepts, namely H-C-Semi compact and H*- C-Semi compact in bitopological space several propositions and examples about these concepts are introduced .

In this paper, we define another types of Lindelof on bitopological space, namely N-Lindelof , S-Lindelof and pair-wise Lindelof spaces, and we introduce some properties about these types.

In this paper we study some results concerning the existence of splitting fields which are generated by roots of polynomials. Also we study the roots of cubic polynomials.

Actually, it is possible to solve systems of integral equation by using many approaches. However, in this study, the modified quasi Simpson's 3/8 rule used to find the numerical solution of a system of linear Volterra integral equations of the second kind. This method solves systems of linear Volterra integral equations of the second kind in more accurate way than the modified trapezoidal rule. Some indicative examples are given to elaborate the accuracy of this method .

ABSTRACT: It was found in this research that the mineral waters contain high concentrations
of TDS which was confirmed by high values of their conductivities which were directly
proportional to their TDS. The levels of TDS in Found waters were not controlled in time of
preparation of these waters. The adsorption of Berlent Green dye on Bentonite Clay surface
decreased with increasing temperature which was confirmed by negative values of enthalpies and
free energies in addition to negative valves of entropies which rendered the system most
regulation. The adsorption follows Frendlich isotherm and the adsorption increased with
decreasing pH which reached its maximum value at pH=1.There fore, these waters should be
defected from time to insure their suitability for drinking and their contents within the water
Health Organization specifications and using(U.V-visible) Instrument, (pH-meter) for
measurements.

:Differential Operators (Gradient, Laplacian and Biharmonic) have been used to
determine anomaly characteristics using theoretical gravity field for prismatic bodies with different
top depths, dimensions and density contrasts. The concepts of gradient and laplacian operator are
widely used in image processing. The intersection between the gravity field and the three differential
operator's fields could be used to estimate the depth to the top of the prismatic bodies regardless of
their differences in dimensions, depths and density contrasts. The Biharmonic Operator has an
excellent result, were two zero closed contour line produced. The outline of the internal closed zero
contour line define precisely the dimension of the prismatic bodies. The distance between this zero
contour and the maxima of the Laplacian Operator define the exact depth to the top of the prismatic
bodies. The maxima of the Biharmonic amplitude could be used for density contrast approximation.
This is the first attempt to use such technique for estimating body characteristics. Also, the
Biharmonic Operator has high sensitivity to resolve hidden small anomaly due the effect of large
neighborhood anomaly, the 2nd derivative Laplacian Filter could reveal these small anomaly but the
Biharmonic Operator could indicate the exact depth. The user for such technique should be very care
to the accuracy of digitizing the data due to the high sensitivity of Biharmonic Operator. The validity
of the method is tested using field example for salt dome in Gulf Coast basin

:The area located between Al-Khosar river catchment and Bashiqa anticline differs
morphologically and lithologically .The study showed that there are differences in the slopes of these rocks
caused by structural and denudational factors .Geomorphological analyses to the prevailing flow net have been
carried out and the results show that the region is stable with bifurcation ratio ranging between (3.1-4 )and
drainage density of (1.45 Km-1 ). Moreover a comprehensive field survey was performed taking into consideration
the important groundwater wells in the area in order to give an evaluation of water quality Most of these wells
penetrate Injana Fm .With depth less than 30m .But few of them penetrate deeper Al-Fatha Fm .Of more than(
150m).Fifteen groundwater samples were collected from the whole area on three stages , i.e .March , June , and
September 2010 and appropriate Laboratory analyses were done .The results shown in the tables represents the
mean of the three readings .The study also revealed that groundwater in the area is not suitable for drinking due to
a high concentration of dissolved solids (T.D.S ).which frequently exceed the world standard limits while a high
concentrations of (Na +, SO4=), and electrical conductivity have been observed in the south and southwest
direction very close to Al-Khosar river catchment .In general the groundwater of most wells in the area could be
considered suitable for Irrigation purpose .

The aim of this study is to improve the static equation of the possibility of seeing the crescent.
The factors (age of the moon, moon altitude, anomaly of the sun, the duration of stay Crescent (mukth)) for
many years from (2004 to 2013 A.D) has been calculated and used to improve the equation of the probability
of sighting the crescent. We find through the analysis of these factors and compared with different astronomical
criteria's that the results we obtained were more accurate and in a good agreement with other criteria.
y = 5.3295x + 5.3396
-20
-10
0

A seismic refraction investigation was carried out at Al-Aziziyah area for delineating water table and determining the velocity of different layers, as well as locating the contamination zones in under ground water which are resulted from agricultural activities. Nineteen seismic profiles were carried out in this study using ABEM TERRALOC seismic system. The length of each profile ranged from (33-115) m. with normal and reverse shooting. The recorded field data interpreted by delay time method using time distance curve as well as plus-minus method, mean-minus time method, ABC method and generalized reciprocal method. The velocities of the first layer range from (252-404) m/sec. and its average is (295.53) m/sec. while the velocities of the second layer ranges from (1335-1666) m/sec. and its average is (1494.21) m/sec. the velocities show no significant difference after interpreting by different methods. In this study the second layer represents the water table in the area with the depths range from (2.2- 5.5) m. Finally the contour map was drawn for the water table of the ground water, as well as a map differentiating the contaminated from non-contaminated zones.

جرت هذه الدراسة فی مختبرات قسم الفیزیاء وقسم علوم الحیاة فی کلیة التربیة جامعة تکریت لمعرفة تأثیر نوعین من التشعیع ү-ray , X-ray وفترات تعریض مختلفة (1,2,3,6,9,12,15) ثانیة فی قتل البکتریا (Staphylococcus aureus) وقد جرى حضن البکتریا بعد التشعیع لفترات زمنیة (2,4,24) ساعة حضانة وکانت النتائج التی حصلنا علیها تفوق أشعة کاما بالمقارنة مع X-ray معنویا فی قابلیتها على قتل المزارع البکتیریة وان أفضل فترة لتعریض هذه المزارع البکتیریة إلى الإشعاع کانت (12)ثانیة والتی أعطت أعلى قدرة على قتل أو الحد من أعداد المزارع البکتیریة کما أن تقدم فترة الحضن أدت إلى استعادة البکتریا لنشاطها وهذا بسبب أمکانیة البکتریا فی أصلاح مادتها الوراثیة الحیة بعد التشعیع وبعد فترة حضانة مستمرة نستنتج أن أشعة کاما أفضل من أشعة X-ray فی أمکانیة قتل أو الحد من أعداد المزارع البکتیریة کما أن فترة التعریض للبکتریا (12) ثانیة هی أفضل فترة لقتلها .