Firas Taher Maher
Abstract
The present study describes the effect of phosphate ester of paracetamol as aprodrugs on choline estarse was partial purified from serum of patients with diabetes type two and the effect ...
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The present study describes the effect of phosphate ester of paracetamol as aprodrugs on choline estarse was partial purified from serum of patients with diabetes type two and the effect of the prodrug in some enzyme activity In vivo . This study devided two parts : 1- In vitro study : choline estarse was partial purified from serum of patients with diabetes type two .The kinetics of the enzyme were studied and the results showed that the maximum velocity was 13 IU / ml and the Km was 0.066 M while the optimum temperature of the enzyme was 370C and the optimum pH was 7.4.The effect of this new prodrug was tested on the activity of choline estarase . Result showed that the compound has inhibitory effect on the enzyme activity and that the degree of inhibition increases when the concentration of the compound was increased. The type of inhibition was non-competitive. 2- Invivo study showed highest concentration of parcetamol drug released from prodrug at 6hr. after giving the dose to the animal . The results showed an increase in the activity of ( AST, ALT, LDH, ALP,, GGT and ChE) and a decrease in the concentrations of TG, LDL-c, VLDL-c, Cholestrol while there was an increase in HDL-c level.
