ANAS ABDULLAH HAMMAD; Hana Abdullatif Yassin
Abstract
The study included the collection and examination of 480 of sample from blood bags components of stored in the main blood bank in Ramadi, The isolates were identified isolated from ...
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The study included the collection and examination of 480 of sample from blood bags components of stored in the main blood bank in Ramadi, The isolates were identified isolated from blood bags components depending on the morphological and microscopic tests and also on the Biochemical tests in order to identify bacterial species and to ensure the primary characters ; 68 isolates were recognized with a percent of 85.29% for the gram positive bacteria. They were related to four types, Staphylococcus epidermidis which gave 28 isolates with a percent of 41.17% , 7 isolates related to Staphylococcus aureus with a percent of 10.29% , 14 isolates related to Staphylococcus haemolyticus with a percent of 20.58% , and 9 isolates related to Bacillus cereus with a percent of 13.23% . gram negative bacteria included 10 isolates related to two bacterial types with a percent of 14.20%. The first 6 isolates related to Klebsiella pneumoniae with a percent of 8.82% and second type included pseudomonas aeruginosa 4 isolates with a percent of 5.88The sensitivity of these isolates to 15 antibiotics was tested ; the resistance percent of the gram positive bacteria isolated from blood bags components was 63.12% to all antibiotics used . The resistance percent was 100% Penicillin , 92.5% ,95% to Ampicillin and Erythromycin respectively, Amikacin and Gentamycin had a high activity against isolates and reduced their resistance to22.5% ,17.5% respectively, and also Chloramphenicol reduced their resistance to 40% . The resistance percent for gram negative bacteria was 76% to all antibiotics used,100% against Penicillin, Ampicillin , Erythromycin and Rifampin, 90% against Co-trimoxazole, Tetracyclin and Ciprofloxacin Cefotaxime and Ceftriaxon 70%, finally50% ,60% and 50% against Amikacin , Chloramphenicol and Gentamicin respectively. All isolates were positive and negative gram sensitive100% to the antibiotic Imipenem .The blood bags platelets showed highest contamination was 23.75%,The lowest contamination was in the bags of red blood cells 3.75% . The minimal inhibitory concentrations (MICs) of some disinfectants were also determined . The low concentrations of Isopropyl-alcohol(2- 64mg/ml) the most effective as compared to others to inhibit bacterial growth. However, Hibitans at 256- 3500mg/ml had the lowest effects on the inhibition of bacterial growth.
