Journal of University of Anbar for Pure Science

Detection of Tetracycline Resistant Gen (tet K, tet M ) in Some Coagulase Negative Staphylococci Isolated From Different Clinical Sources In Erbil City

Khadija Kh. Barzani; Zheen A. Abdullah; Soma P. Hydaeat; Asia S. Moohamad

Volume 10, Issue 1 , April 2016, , Page 11-20

https://doi.org/10.37652/juaps.2016.132437

Abstract

Thirty two isolates of coagulase negative staphylococci was isolated and identified from clinical sources ; including eight isolates (12.5%) of each of Staphylococcus hominis and Staphylococcus ... Read More ... Thirty two isolates of coagulase negative staphylococci was isolated and identified from clinical sources ; including eight isolates (12.5%) of each of Staphylococcus hominis and Staphylococcus haemolyticus from (64) patients urine which suffering from urinary tract infection and eight isolates (16%) of each of Staphylococcus epidermidis and Staphylococcus auricularis from (50) ear patients which suffering from otitis in Rizgary teaching hospital in Erbil city were isolated depending on Vitek 2 compact system . The susceptibility tests by using disc diffusion agar method were determined for all isolated coagulase negative Staphylococci to 10 different antibiotics . From obtained results it was observed that all isolates were 100% sensitive to ceftriaxone and novobiocin, while higher resistance was obtained to tetracycline with percentage 50% and they showed differnt resistant to other remaining antibiotics. On the other hand, all isolated Staphylococcus hominis, Staphylococcus haemolyticus , Staphylococcus epidermidis and Staphylococcus auricularis isolates were tested for tetracycline resistant genes tetK and tetM by using PCR and the results showed that 75% of Staphylococcus hominis, Staphylococcus haemolyticus and Staphylococcus epidermidis were carried tetM gene with product size about 158 bp while 50% of Staphylococcus auricularis was carried the above mentioned gen . Also it was found that 50% of Staphylococcus hominis and Staphylococcus haemolyticus isolates in this study were harboring tetK gene with product size 360 bp and no tetK gene was observed in Staphylococcus epidermidis and Staphylococcus auricularis . Also the antibacterial effect of two local species of menthes against resistant isolated bacteria by using the Minimum Inhibitory Concentration (MIC) method were studied . The results showed that the MIC for aqueous extract for both plants Mentha longifolia and Mentha piperita were 1600µg/ml and it were 1400µg/ml for alcoholic extract of both plants against most Staphylococcus speciese except Staphylococcus epidermidis which the MIC was 1200 µg/ml for aqueous and 1000µg/ml for alcoholic extract of Mentha piperita, while it was 1200µg/ml for both the aqueous and alcoholic extracts for Mentha longifolia.

دراسة التباین الجزیئی للعزلات المرضیة لبکتریا الزوائف الزنجاریة لجینات ,phzM , toxA ,pvdE 16srRNA الخاصة بالضراوة والصفات التشخیصیة

Ahmed M. Yuosif; Ahmed M. Turkey; Ahmed A. Suliaman

Volume 9, Issue 3 , December 2015, , Page 24-35

https://doi.org/10.37652/juaps.2015.127584

Abstract

This study includes collection of 438 clinical samples from Ramadi Educational Hospital and 50 of soil samples in period from Nov. 2014 to Feb. 2015, to isolate Pseudomonas aeruginosa ... Read More ...

Molecular Variation Study of Clinical Isolates of Pseudomonas aeruginosa for 16SrRNA,pvdE, toxA, and phzM Genes Related with Virulence and Characterization Features.

Ahmed M. Yuosif; Ahmed M. Turky; Ahmed A. Sulaiman

Volume 9, Issue 3 , December 2015, , Page 144-155

https://doi.org/10.37652/juaps.2015.127638

Abstract

Using Multiplex PCR Technique for Detection of Some Pathogenic Gram Positive bacteria and Determination of Its Antibiotic Resistance

Ahmed M. Turkey; Ahmed A. Sulaiman

Volume 9, Issue 2 , August 2015, , Page 73-80

https://doi.org/10.37652/juaps.2015.127656

Abstract

450 samples were collected from different pathological cases (urine, wounds, Burns, stool, and nasal and pharyngeal swabs) from November 2014 through February 2015. 60 samples were ... Read More ...

Use Multiplex PCR For Detection of Some Virulence Genes of Klebsiella Isolated From Different Environmental loci

Zena J. Mohammed; Ali H. Abdulkareem; Ahmed A. Sulaiman

Volume 9, Issue 1 , April 2015, , Page 60-66

https://doi.org/10.37652/juaps.2015.124384

Abstract

This study includes collected of 399 samples from different sources included clinical and non clinical samples. 293 clinical samples (urine, wounds, burns, stool, sputum). Non- clinical ... Read More ...

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Keywords = Antibiotic resistance

Detection of Tetracycline Resistant Gen (tet K, tet M ) in Some Coagulase Negative Staphylococci Isolated From Different Clinical Sources In Erbil City

Abstract

دراسة التباین الجزیئی للعزلات المرضیة لبکتریا الزوائف الزنجاریة لجینات ,phzM , toxA ,pvdE 16srRNA الخاصة بالضراوة والصفات التشخیصیة

Abstract

Molecular Variation Study of Clinical Isolates of Pseudomonas aeruginosa for 16SrRNA,pvdE, toxA, and phzM Genes Related with Virulence and Characterization Features.

Abstract

Using Multiplex PCR Technique for Detection of Some Pathogenic Gram Positive bacteria and Determination of Its Antibiotic Resistance

Abstract

Use Multiplex PCR For Detection of Some Virulence Genes of Klebsiella Isolated From Different Environmental loci

Abstract