Muthanna H. Hassan; Hassan F. Lahig; Luma A. Yassir
Abstract
The present study included the collection of 120 samples from various clinical sources. The samples collected from some hospitals in Baghdad city which included burns ,wound , cystic ...
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The present study included the collection of 120 samples from various clinical sources. The samples collected from some hospitals in Baghdad city which included burns ,wound , cystic fibrosis, ear infections and urinary tract infections ( 52 ,28, 4, 23 and 13). Samples respectively. Samples were identified based on the morphological and microscopical characteristics of the colonies when they were culturing in a number of culture media as well as biochemical tests. The molecular detection was used to confirm diagnostic test for the isolates based on the 16SrRNA gene, as a detection gene, which has specific sequences for DNA of P.aeruginosa bacteria.The results of molecular detection showed that 51 isolates belong to target bacteria were distributed as follow 21 of burns, 14 isolates of wounds, 7 isolates of ear infection, 4 isolates of cystic fibrosis, 5 isolates of urinary tract infection. The results of phenotypic detection of biofilm formation showed that 44/51 (86.2%) of isolates differences in their ability to biofilm formation. The results of screed QS genes by Polymerase chain reaction (PCR) amplify based on specific sequences for lasI gene showed that 48 (94.1%) of isolates have lasI gene.
