Document Type : Research Paper

Author

Mosul University - College of Education

Abstract

Twenty bacterial were isolated of Escherichia coli from children with urinary tracts infection. These isolates were identified by cultural and biochemical tests and Antibiotic susceptibility tests were carried out to determine their resistance patterns so they were 75%, 100%, 50%, 100%, 80%, 90%, 75%, 60%, 100% and 35% resistant to Ampicillin, Amoxicillin, Neomycin, Kanamycin, Streptomycin, Tetracycline, Nalidixic acid, Ciprofloxacin, Erythromycin, Norefloxacin respectively

Main Subjects

1-Patwary, A.K. (1994). Multidrug resistance shigella infections in children. J. Diarrhoel. Dis. Res. 12: 182-186.
2-Hardy, K.G.; Plasmids. (1993). Oxford University press, Oxford.
3-Winkur, P.L.; Vonstein, D.L.; Hoffman, L.J.; Uhlenhoop, E.K.; Doern, G.V. (2001). Evidence for transfer of CMY-2 AMPC-lactamase plasmids between E.coli and salmonella isolates in food, animals and humans. Antimicrob. Chemother., 45(10): 2716-2722.
4-Yah, S.C.; Eghafona, N.O.; Enabulele, I.O. and Aluyi, H.S.A.(2006). Ampicillin usage and ampicillin resistance (Ampr) plasmids mediated E. coli isolated from diarrheagenic patients attending some teaching hospitals in Nigeria. Shinaz emedical Journal J., 7 (4): 15-30.
5-Kunin, C.M. (1994). Urinary tract infections in females. Clin. Infect. Dis. 18:1-12.
6-Koneman, E.W.; Allen, S.D.; Janda, W.M.; Schreckenberger, P.C. and Winn, W.C. (2007). Colour Atlas and textbook of Diagnostic Microbiology 5th ed., Lippincott Raven Publishers, Philadelphia, USA.
7- Ramos, J. and Levesque, R. (2006). Pseudomonas, Springs, Netherland.
8-Vandepitte, J.; Verhaegen, J.; Engback, K.; Rohner, P.; Piot, P. and Heuck, C.C. (2003). Basic laboratory procedures in clinical bacteriology. World Health Organization, Geneva.
9-Brinboiom, H.C.; Doly, J. (1979). Rapid alkaline extraction procedure for screening recombianant plasmid DNA. Nucleic Acid Res., 7:1513-1524.
10-Meyer's; J. A.; Sanchez, D.; Elewell, O., Falkow, (1976). Simple agrose gel electrophoretic method. For identification and characterization of plasmid deoxy ribonucleic acid, J. Bacteriol. 127: 1529-1537.
11- LIU Yan, ZHANG Bi-Li, WANG Wen-Hong, ZHANG Xuan, FAN Shu-Ying, LI Li, (2011). Antibiotic resistance of pathogens isolated from 181 children with complicated urinary tract infectionVol. 13 Issue (5): 381-384    
12-Azad, A. K., Shahjahn, M. (1999). Molecular characterization of chloramphenicol resistance gene in Escherichia coli for urinary tract infections. M. Sc. Thesis, Department of Biochemistry, University of Rajshahi, Bangladesh.
13-Martinez, L. Y.; Arenas, MMp., Montes, MYR., Martinez, L. J., Baca, BE. (1987). Antibiotic resistance and plasmid pattern of enter toxigenic ST-a Strains of Escherichia Coli isolated in Puebla, mexico, Can. J. Microbiology , 33: 816- 819.
14-Sharif, F.A.; Astal, Z.Y. (2004). Role of plasmids in mediating antibiotic resistance and extended- spectrum beta- lactamase production in Escherichia coli. J. Islamic University of Gaza 12(2): 1-8.
15-Shehabi, A.A. Mahafzah, A.M. and AL- Khalili, K.Z. (2004). Antimicrobial resistance and plasmid profilies of urinary Esherichia coli isolates from Jordanian patients. East. Mediterr. Health J., 10(3): 322- 328.
16- Seow Ven Lee and Abdul Rani Bahaman (2012), Discriminatory Power of Agarose Gel Electrophoresis in DNA Fragments Analysis , Putra University Malaysia.
17-Kapil, A. (2005). The chellenge of antibiotic resistance: need to contemplate Indian J. Med. Res. 121: 83-91.
18-Gales, A.C. (2000). Activity and spectrum of 22 antimicrobial agents tested against urinary tract infection pathogens in hospitalized patients in latin America report from second year of sanitary antimicrobial surveillance program. (1983). J. Antimicrob. Chemother., 45: 295-303.