HALA. M. MAJEED; HASSAN. A. ABDUL. RATHA
Abstract
This study was conducted to compare the inhibitory effect of lactic acid bacteria and its filtrate which were concentrated two times and half non-concentrated filtrate with some antibiotics ...
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This study was conducted to compare the inhibitory effect of lactic acid bacteria and its filtrate which were concentrated two times and half non-concentrated filtrate with some antibiotics which used usually as therapeutic against Staphylococcus aureus growth. Three lactic acid bacteria isolates were obtained for this goal from microbiology dept. laboratory, Vet. College - Baghdad university and these three isolates were lactobacillus casei (v1), L. casei (v2) and L. fermentatum .Sixty hundred and two milk samples were collected from 358 cows infected with acute and sub clinical mastitis distributed between Zob'aa village in Abu-Ghraib and Al-Naser station of cows in Al-sewera city. Samples were cultured on blood base agar and Manittol salt agar, biochemical tests were performed to ensuring the results of diagnosis Diagnostic study depending upon morphological, cultural and biochemical tests yielded the isolation of 173 staphylococcus aureus isolate.The susceptibility tests showed that S. aureus isolates varied on their sensitivity towards antibiotics , all these isolates were found to be resistante by the percentage 100% to Ampicillin ,Lincomycine and vancomycin and 94,8% , 47,4% to pencillin-G and chloromphenicol respectively while these isolates were found to be susceptible 100%, 90,75% , 76,88% , 72,26% and 71,1% to ciprofloxacin, Kanamycin, Gentamycin, Sulfatri metheprime and tetracycline respectively. The three Isolates of lactic acid bacteria varied on their inhibitory effect against S. aureus Isolates L. casei (v1) showed their inhibitory effect against S. aureus isolates which were sensitive to ciprofloxacin, kanamycin, Gantamycin, Sulfa tri metheprime, Tetracycline, Penicillin-G, Chloramphenicol with inhabitation zone of 13mm while the zone increase to 18mm with the same isolates L. casei (v1) against S. aureus which resist to antibiotics except Ciprofloxacin. L. casei (v2) gave inhibitory zone of 19mm against S. aureus isolates sensitive to antibiotics and 20mm for S. aureus isolates resist to antibiotics isolates of L. fermentum differed in their inhibitory effect between observed effect 15mm against S. aureus which were sensitive antibiotics to more effect against the same isolates 17mm which were resist to antibiotics. Filtration of lactic acid bacteria isolates growth showed good inhibitory effect range between 18-20mm against S. aureus which were sensitive to antibiotics and 19-22mm against the same isolates which were resist to antibiotics.Concentration filtrate of lactic acid bacteria showed an increase of the inhibitory zone (23,24,23) mm for L. casei(v1) , L. casei(v2) , and L. fermentum respectively after concentrated in compare with (19,22,20)mm before concentrated
