KHALAF JASIM MOHAMMED AL-DOLAIMI
Abstract
The bacterium Kl. pneumoniae was isolated from urine sample of patient with urinary tract infection and diagnosed according to its cultural and microscopic characters and biochemical ...
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The bacterium Kl. pneumoniae was isolated from urine sample of patient with urinary tract infection and diagnosed according to its cultural and microscopic characters and biochemical reactions . Sensitivity test of the bacterium against antibiotics was established and the results appeared that the bacterium was Sensitive to Amoxycillin , Gentamycin , Cephaloxin, Neomycin, Rifampicin , Chloramphenicol , Tobramycin , Ampicillin and carpenicillin and resistant to clindomycin ,cloxacillin , Trimethprim , Cefalotine , Vancomycin , Penicillin G , Lincomycin , Fusidic acid , Metronidazole and Nalidixic acid . Results of plasmid DNA content for the bacterium showed the presence of 8-6 kb plasmid compared with the plasmid pBR 322 isolated from E. coli HBlol . To study the role of plasmids in coding for antibiotics resistance , curing experiment was accomplished using SDS .Results showed the loss of resistance of the antibiotic , Nalidixic acid after physical loss of plasmid as ensured by gel electrophoresis.
