Journal of University of Anbar for Pure Science

Evaluation of Garlic efficiency on precipitated lead chloride in hepatocytes of mice

Sumaiah Ibrahim Hussein; Khalid Hamdan Gathwan; Muna Sachit Hashim; Sara Majed Obaid

Volume 17, Issue 2 , December 2023, , Page 12-18

https://doi.org/10.37652/juaps.2023.141429.1092

Abstract

AbstractLike other soluble lead compounds, exposure to lead chloride may cause lead poisoning, In the current study, we seek to determine the effect of lead chloride on body and liver ... Read More ...

Molecular Detection of Pseudomonas aeruginosa and Study the Effect of Fresh Garlic juice on Some Virulence Factors of this Bacteria.

Hassan F. Lahig; Muthanna H. Hassan; Luma A. Yassir

Volume 11, Issue 2 , August 2017, , Page 9-17

https://doi.org/10.37652/juaps.2017.141584

Abstract

The present study included the collection of 169 samples from various clinical and environmental sources for investigation of existence of Pseudomonas aeruginosa in those sources, the ... Read More ... The present study included the collection of 169 samples from various clinical and environmental sources for investigation of existence of Pseudomonas aeruginosa in those sources, the clinical samples collected from some hospitals in Baghdad city and included 126 samples of wound, burns, cystic fibrosis, ear infections and urinary tract infections (35, 52,4, 22 and 13) samples respectively, while environmental samples were distributed by 25 samples from soil, and 18 samples from wastewater and some swamps. Samples were identified based on the morphological and microscopical characteristics of the colonies when they were culturing in a number of culture media as well as biochemical tests. The molecular detection was used to confirm diagnostic test for the isolates based on the 16SrRNA gene, as a detection gene, which has specific sequences for DNA of P.aeruginosa bacteria.The results of molecular detection showed that 51 isolates belong to target bacteria were distributed as 20 of burns, 9 isolates of wounds, 7 isolates of ear infection, 4 isolates of cystic fibrosis, 2 isolates of urinary tract infection, 5 isolates of soil and 4 isolates of water. The results of phenotypic detection of some virulence factors showed that 49 isolates with percent (95%) produced beta-hemolysis, while 46 isolates (90%) showed variance in the strength of pycocyanin production with a absorbance ranging from (0 to 0.52) at 690 nm, and 47 (92%) of isolates were producing protease enzyme, 44 (86%) of the isolates showed a differences in their ability to biofilm formation. As a result of the increased resistance of P.aeruginosa bacteria towards antibiotics, it is became a problem in the treatment of the infectious diseases caused by this bacteria therefore, the current study focused on the use of fresh garlic extract towards of some virulence factors because it being easy and commonly used. The results of the statistical analysis showed a significant difference when P-value (<0.05) in the production of some virulence factors, the isolates lost their ability to form biofilm, and decreased their ability to produce protease enzyme, and pyocyanin pigment, while did not affect the production of hemolycin.

Determination of Alliin and Allicin in different types Garlic using High Performance Liquid Chromatography

Mohammad J. Abdul Ghani

Volume 4, Issue 2 , August 2010, , Page 16-23

https://doi.org/10.37652/juaps.2010.15391

Abstract

Abstract:Alliin and Allicin products were measured and determined by ion-pair reversed-phase liquid chromatography (RP-LC) with UV detection at 210 nm. These two Compounds were extracted ... Read More ...

THE USES OF GARLIC AS ANTIBACTERIAL IN VITRO

Abeer Yousif Abdul-Kareem

Volume 2, Issue 3 , December 2008, , Page 1-15

https://doi.org/10.37652/juaps.2008.15594

Abstract

The aim of this study was to determined the inhibitory effect of different concentrations of garlic extract in vitro. Thirty clinical isolates of Staphylococcus aureus were obtained ... Read More ...

Journal of University of Anbar for Pure Science

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Keywords = Garlic

Evaluation of Garlic efficiency on precipitated lead chloride in hepatocytes of mice

Abstract

Molecular Detection of Pseudomonas aeruginosa and Study the Effect of Fresh Garlic juice on Some Virulence Factors of this Bacteria.

Abstract

Determination of Alliin and Allicin in different types Garlic using High Performance Liquid Chromatography

Abstract

THE USES OF GARLIC AS ANTIBACTERIAL IN VITRO

Abstract