Journal of University of Anbar for Pure Science

Produce the single cell protein (SCP) by the solid culture method from Schanginia aegyptiaca wastes with Aspergillus niger and use in feeding of carp fish (Cyprinus carpi)

Ahmed Sh. A Lafi; Daffer F. Al-Rawii; Abdullah Kh. Al-issa; Idham A. Assaffii

Volume 3, Issue 1 , April 2009, , Page 23-33

https://doi.org/10.37652/juaps.2009.15563

Abstract

The study was conducted to produce the single cell protein and to increase the content of Schanginia aegyptiaca wastes from the parent microbial protein was to grow isolates from (Aspergillus ... Read More ... The study was conducted to produce the single cell protein and to increase the content of Schanginia aegyptiaca wastes from the parent microbial protein was to grow isolates from (Aspergillus niger) by the use of nutrient media of Schanginia aegyptiaca powder and wastes of dairy factories and dates wastes by using incubators and solid fermentation. The suitable conditions for production such as components media, percentage of support materials, time of incubation and best isolate for production were determined. Yield components of single cell protein were checked. The yield, was then used in specified ratios of replacement for the vegetable protein in the preparation of carp fish (Cyprinus carpi) food. The results were as follows:- 1-Obtaining 10 local isolates inhabiting Schanginia aegyptiaca, three of them were selected depending on the ratio of growth diameter which ranged between (5.60-6.40) cm on PDA media, The Is-8 isolate has been selected and diagnosed to belong to fungi A. niger-P8 with A. niger-S isolate. 2-Wetting the powder media of Schanginia aegyptiaca with Whey and extract of dates wastes and fermented in a solid culture doubled the percentage of the protein in the media up to (5) times. The highest percentage 8.14% was obtained when wetting withWhey and by the use of A. niger-P8 isolate. 3-The produced protein contained low percentage of nucleic acids RNA and DNA. The totals of their percentage were 5.18% and 5.32% in liquid culture protein, and 3.10% and 3.72% in solid culture protein for A. niger-P8 and A. niger-S isolates, respectively, revealed that production are free from toxins. 4- The microbial protein produced contained 15 amino acids. The rates of the amino acids were 64.95 and 63.39 g/100g of protein, the analysis of the microbial protein showed the highest content of Aspartic; 14.29 and 12.32 g/100g of protein for A. niger-P8 and A. niger-S isolates, respectively. 5-The percentage of replacement 50% of Soya bean with the microbial protein A. niger-P8 produced gave the highest rate in the weight of carp Cyprinus carpi 26.22 g per fish after 35 days of feeding with a difference 5.7% compared to control treatment.

Produce the single cell protein (SCP) by the liquid cultures methods from Schanginia aegyptiaca powder by the use Aspergillus niger and testing Biological

Samir Sarhan Khalil; Dhafer Fakri Al-Rawii; Ahmed Shehab Ahmed lafi; Adham Ali Al-Assaffii

Volume 2, Issue 2 , August 2008, , Page 117-129

https://doi.org/10.37652/juaps.2008.15349

Abstract

The study was conducted to produce the single cell protein by the use of Schanginia aegyptiaca powder wastes of nutrient media and wheat hey. The aim was to grow local isolates which ... Read More ... The study was conducted to produce the single cell protein by the use of Schanginia aegyptiaca powder wastes of nutrient media and wheat hey. The aim was to grow local isolates which were already diagnosed and sieved from (Aspergillus niger) in the liquid cultures methods by using flasks in shaker incubators fermentation. The suitable conditions for production such as components media, percentage of support materials, time of incubation and type of best isolate for production were determined. Yield components of single cell protein were checked. The yield, was then used in food and the breeding media of fruit fly (Drosophila melanogaster). The results were as follows :- 1- Obtaining 10 local isolates inhabiting Schanginia aegyptiaca, three of them were selected depending on the ratio of growth diameter which ranged between (5.60-6.40) cm on PDA media. 2- The selected isolates Is-3, Is-4, Is-8 with A.niger-S showed an ability to grow on the prepared media Schanginia aegyptiaca with a rate lower than the PDA media. The Is-8 isolate has been selected and diagnosed to belong to fungi A. niger-P8 with A.niger-S isolate. 3- The liquid media, prepared from the powder of Schanginia aegyptiaca and wheat hey in 1:1 percentage produced the highest yield of single cell protein 9.11 g/Lit with A. niger-P8 isolate and 8.91 g/Lit with A. niger-S isolate during 8 days incubation period. 4- The highest percentage of the parent protein in biomass was 32.12 and 32.56 from A. niger- P8 and A. niger-S isolates, respectively, and the produced protein contained low percentage of nucleic acids RNA and DNA. The totals of their percentage were 5.18% and 5.32%, and revealed that it contains 15 amino acids. The rates of amino acids were 81.4 and 80.5 g/100g of protein for the two isolates A. niger-P8 and A. niger-S, respectively, and characteristically the highest content of Aspartic; 16.12 and 15.62 g/100g of protein in the liquid cultures for the A. niger-P8 and A. niger-S isolates, respectively. revealed that production are free from toxins. 5- The use of microbial protein produced from the liquid fermentation for A. niger-P8 and A. niger-S in feeding fruit fly Drosophila melanogaster instead of Corn starch in 100% percentage gave the highest rate in the number of mature insect 1473 and 1381, for the proteins of the two isolates A. niger-P8 and A. niger-S, respectively.

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Keywords = Aspergillus niger

Produce the single cell protein (SCP) by the solid culture method from Schanginia aegyptiaca wastes with Aspergillus niger and use in feeding of carp fish (Cyprinus carpi)

Abstract

Produce the single cell protein (SCP) by the liquid cultures methods from Schanginia aegyptiaca powder by the use Aspergillus niger and testing Biological

Abstract