Journal of University of Anbar for Pure Science

Detection of genetic diversity for number of Iraqi maize cultivars (Zea mays L.) by using the RAPD-PCR technique

Muataz A. Farhan; Abdul Majeed A. Hamadi

Volume 10, Issue 3 , December 2016, , Page 30-38

https://doi.org/10.37652/juaps.2016.134880

Abstract

Abstract:In this study six varieties of maize (Zea mays L.) used which where Z. mays saccharata (Z1), Z. mays indurate (Z2), Z. mays indentata (Z3), Z. mays everta (Z4), Z. mays tunicate ... Read More ...

GENETIC DIVERSITY ANALYSIS FOR NUMBER OF DATE PALM VARIETIES IN AL-ANBAR USING THE RAPD-PCR TECHNIQUE

KHALID JAMAL HASHIM; ABDUL MAJEED A. HUMADI

Volume 8, Issue 2 , August 2014, , Page 31-39

https://doi.org/10.37652/juaps.2014.123975

Abstract

The present study deals with the application of RAPD markers to study ten varieties of date palm in order to determine the genetic relationship and genetic distance among them. also ... Read More ...

Molecular characterization of Pseudomonas aeruginosa Isolates Isolated from Clinical Patients by Using RAPD-PCR Technique.

Akeel H. AL-Assie; Adnan F. Al-azawy; Wakas S. Al-Dori

Volume 6, Issue 3 , December 2012, , Page 27-35

https://doi.org/10.37652/juaps.2012.78439

Abstract

The aim of the present study was the molecular characterization and the evaluation of variability and genetic relationship of six Pseudomonas aeruginosa isolates using PCR-based Randomly ... Read More ... The aim of the present study was the molecular characterization and the evaluation of variability and genetic relationship of six Pseudomonas aeruginosa isolates using PCR-based Randomly Amplified Polymorphic DNA (RAPD) technique. A total number of 86 samples were collected from patients that hospitalized in Tikrit Teaching Hospital in Tikrit city. These samples were taken from patients basing on the sources of infections, the isolates were taken from: wounds, ear, burns, urine, sputum, and eyes infections. Using enrichment, selective media, and biochemical tests, that characterized and identified as P. aeruginosa. Genomic DNA was extracted from six P. aeruginosa isolates isolated from these different sources. These genomic DNA samples were found to have a suitable concentration and purity for RAPD-PCR analysis. RAPD-PCR technique was performed using 15 different Operon random primers. Eleven primers gave successful amplification results in repeated experiments. As a result, the total number of amplified bands was 270 and the total number of polymorphic bands was 234. The highest number of polymorphic bands (39 bands) was produced by primer OPX-01. The primer efficiency ranged from 3.70 (primer OPA-11) to 14.44 (primer OPX-01) and the discriminatory value ranged from 1.70% (primer OPA-11) to 16.66% (primer OPX-01). In addition, genetic distance and cluster analysis among different P. aeruginosa isolates were estimated by using UPGMA computer program basing on RAPD-PCR banding patterns that obtained in this study. These results suggesting that possible and frequent occurrence of mutations in DNA sequencing P. aeruginosa bacteria from different sources and locations. This study has proved existence genetic differences (DNA polymorphism) among the six P. aeruginosa isolates isolated from different sources. Therefore, we can say that RAPD technique could be an efficient technique for studying the molecular characterization and the epidemiology of P. aeruginosa bacteria.

Journal of University of Anbar for Pure Science

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Keywords = RAPD

Detection of genetic diversity for number of Iraqi maize cultivars (Zea mays L.) by using the RAPD-PCR technique

Abstract

GENETIC DIVERSITY ANALYSIS FOR NUMBER OF DATE PALM VARIETIES IN AL-ANBAR USING THE RAPD-PCR TECHNIQUE

Abstract

Molecular characterization of Pseudomonas aeruginosa Isolates Isolated from Clinical Patients by Using RAPD-PCR Technique.

Abstract